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Arsenic doping in silicides has been much less studied compared with phosphorus. In this study, superconductivity is successfully induced by As doping in Mo5Si3. The superconducting transition temperature (Tc) reaches 7.7 K, which is higher than those in previously known W5Si3-type superconductors. Mo5Si2As is a type-II BCS superconductor with upper and lower critical fields of 6.65 T and 22.4 mT, respectively. In addition, As atoms are found to selectively take the 8h sites in Mo5Si2As. The emergence of superconductivity is possibly due to the shift of Fermi level as a consequence of As doping, as revealed by the specific heat measurements and first-principles calculations. Our work provides not only another example of As doping but also a practical strategy to achieve superconductivity in silicides through Fermi level engineering.
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BACKGROUND: The molecular mechanisms driving hepatocellular carcinoma (HCC) remain largely unclear. As one of the major epitranscriptomic modifications, N6-methyladenosine (m6A) plays key roles in HCC. The aim of this study was to investigate the expression, roles, and mechanisms of action of the RNA methyltransferase methyltransferase-like protein 16 (METTL16) in HCC. METHODS: The expression of METTL16 and RAB11B-AS1 was determined by RT-qPCR. The regulation of RAB11B-AS1 by METTL16 was investigated by RNA immunoprecipitation (RIP), methylated RIP (MeRIP), and RNA stability assays. In vitro and in vivo gain- and loss-of-function assays were performed to investigate the roles of METTL16 and RAB11B-AS1. RESULTS: METTL16 was upregulated in HCC, and its increased expression was correlated with poor prognosis of HCC patients. METTL16 promoted HCC cellular proliferation, migration, and invasion, repressed HCC cellular apoptosis, and promoted HCC tumoral growth in vivo. METTL16 directly bound long noncoding RNA (lncRNA) RAB11B-AS1, induced m6A modification of RAB11B-AS1, and decreased the stability of RAB11B-AS1 transcript, leading to the downregulation of RAB11B-AS1. Conversely to METTL16, RAB11B-AS1 is downregulated in HCC, and its decreased expression was correlated with poor prognosis of patients with HCC. Furthermore, the expression of RAB11B-AS1 was negatively correlated with METTL16 in HCC tissues. RAB11B-AS1 repressed HCC cellular proliferation, migration, and invasion, promoted HCC cellular apoptosis, and inhibited HCC tumoral growth in vivo. Functional rescue assays revealed that overexpression of RAB11B-AS1 reversed the oncogenic roles of METTL16 in HCC. CONCLUSIONS: This study identified the METTL16/RAB11B-AS1 regulatory axis in HCC, which represented novel targets for HCC prognosis and treatment.
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Carcinoma Hepatocelular , Regulación Neoplásica de la Expresión Génica , Metiltransferasas , MicroARNs , ARN Largo no Codificante , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/patología , Línea Celular Tumoral , Proliferación Celular/genética , Regulación Neoplásica de la Expresión Génica/genética , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/patología , Metiltransferasas/genética , Metiltransferasas/metabolismo , MicroARNs/genética , MicroARNs/metabolismo , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismoRESUMEN
PURPOSE: To develop and internally validate nomograms to help choose the optimal biopsy strategy among no biopsy, targeted biopsy (TB) only, or TB plus systematic biopsy (SB). PATIENTS AND METHODS: This retrospective study included a total of 385 patients who underwent magnetic resonance imaging (MRI)-guided TB and/or SB at our institute after undergoing multiparametric MRI (mpMRI) between 2015 and 2018. We developed models to predict clinically significant prostate cancer (csPCa) based on suspicious lesions from a TB result and based on the whole prostate gland from the results of TB plus SB or SB only. Nomograms were generated using logistic regression and evaluated using receiver-operating characteristic (ROC) curve analysis, calibration curves and decision analysis. The results were validated using ROC curve and calibration on 177 patients from 2018 to 2019 at the same institute. RESULTS: In the multivariate analyses, prostate-specific antigen level, prostate volume, and the Prostate Imaging Reporting and Data System score were predictors of csPCa in both nomograms. Age was also included in the model for suspicious lesions, while obesity was included in the model for the whole gland. The area under the curve (AUC) in the ROC analyses of the prediction models was 0.755 for suspicious lesions and 0.887 for the whole gland. Both models performed well in the calibration and decision analyses. In the validation cohort, the ROC curve described the AUCs of 0.723 and 0.917 for the nomogram of suspicious lesions and nomogram of the whole gland, respectively. Also, the calibration curve detected low error rates for both models. CONCLUSION: Nomograms with excellent discriminative ability were developed and validated. These nomograms can be used to select the optimal biopsy strategy for individual patients in the future.
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Imágenes de Resonancia Magnética Multiparamétrica , Nomogramas , Neoplasias de la Próstata/diagnóstico por imagen , Anciano , Técnicas de Apoyo para la Decisión , Humanos , Biopsia Guiada por Imagen , Modelos Logísticos , Masculino , Persona de Mediana Edad , Antígeno Prostático Específico/análisis , Curva ROC , Estudios RetrospectivosRESUMEN
BACKGROUND: Holobionts comprising nitrogen-fixing diazotrophs and phytoplankton or zooplankton are ubiquitous in the pelagic sea. However, neither the community structure of plankton-associated diazotrophs (PADs) nor their nitrogenase transcriptional activity are well-understood. In this study, we used nifH gene Illumina sequencing and quantitative PCR to characterize the community composition and nifH expression profile of PADs with > 100 µm size fraction in the euphotic zone of the northern South China Sea. RESULTS: The results of DNA- and RNA-derived nifH gene revealed a higher alpha-diversity in the active than in the total community. Moreover, the compositional resemblance among different sites was less for active than for total communities of PADs. We characterized the 20 most abundant OTUs by ranking the sum of sequence reads across 9 sampling stations for individual OTUs in both nifH DNA and RNA libraries, and then assessed their phylogenetic relatedness. Eight of the 20 abundant OTUs were phylogenetically affiliated with Trichodesmium and occurred in approximately equal proportion in both the DNA and RNA libraries. The analysis of nifH gene expression level showed uneven attribute of the abundance and nitrogenase activities by the remaining 12 OTUs. Taxa belonging to cluster III and Betaproteobacteria were present at moderate abundance but exhibited negligible nitrogenase transcription activity. Whereas, the abundances of Richelia, Deltaproteobacteria and Gammaproteobacteria were low but the contribution of these groups to nitrogenase transcription was disproportionately high. CONCLUSIONS: The substantial variation in community structure among active dizatrophic fractions compared to the total communities suggests that the former are better indicators of biological response to environmental changes. Altogether, our study highlights the importance of rare PADs groups in nitrogen fixation in plankton holobionts, evidenced by their high level of nitrogenase transcription.
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Bacterias/aislamiento & purificación , Proteínas Bacterianas/genética , ADN Bacteriano/genética , Nitrogenasa/genética , Plancton/microbiología , Bacterias/clasificación , Bacterias/genética , Bacterias/metabolismo , China , Fijación del Nitrógeno , Filogenia , Agua de Mar/microbiologíaRESUMEN
Ammonium is an important nitrogen sources for plant growth. In this study, we report on the gene characterization of the ammonium transporter AMT1 subfamily in the apple rootstock Malus robusta Rehd. Thirteen AMT genes were comprehensively evaluated from the apple genome (version 1.0). Then the gene features and expression patterns of five AMT1 members from M. robusta were analyzed. These genes fell into four clusters in the AMT phylogenetic tree: clade I (MrAMT1;1 and MrAMT1;3), clade II (MrAMT1;4), clade III (MrAMT1;2), and clade IV (MrAMT1;5). All the AMT1s, apart from MrAMT1;4, were expressed in vegetative organs and strongly responded to nitrogen concentration changes. For example, MrAMT1;2 and MrAMT1;3 had high transcript accumulation levels in the leaves and roots, respectively. Finally, the functions of these AMT1s were studied in detail by heterologous expression in yeast. These genes allowed strain 31019b to assimilate nitrogen, but their 15NH4+ uptake kinetics varied. These results revealed the functional roles of AMT1 during ammonium absorption in the AMT-defective mutant yeast system.
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Proteínas de Transporte de Catión/genética , Malus/genética , Proteínas de Plantas/genética , Secuencia de Aminoácidos , Compuestos de Amonio/metabolismo , Transporte Biológico , Proteínas de Transporte de Catión/metabolismo , Regulación de la Expresión Génica de las Plantas , Prueba de Complementación Genética/métodos , Malus/metabolismo , Nitrógeno/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Raíces de Plantas/metabolismoRESUMEN
Ammonium is the primarily nitrogen source for plant growth, but the molecular basis of ammonium acquisition in fruit species remains poorly understood. In this study, we report on the characterization of two new ammonium transporters (AMT) in the perennial tree Pyrus betulaefolia. In silico analyses and yeast complementation assays revealed that both PbAMT1;3 and PbAMT1;5 can be classified in the AMT1 sub-family. The specific expression of PbAMT1;3 in roots and of PbAMT1;5 in leaves indicates that they have diverse functions in ammonium uptake or transport in P. betulaefolia. Their expression was strongly influenced by ammonium availability. In addition, the transcript level of PbAMT1;5 was significantly affected by the diurnal cycle and senescence hormones. They conferred the ability to uptake nitrogen to the yeast strain 31019b; however, the (15)NH4 (+) uptake kinetics of PbAMT1;3 were different from those of PbAMT1;5. Indeed, PbAMT1;3 had a higher affinity for (15)NH4 (+), and pH changes were associated with this substrates' transport in yeast. The present study provides basic gene features and transcriptional information for the two new members of the AMT1 sub-family in P. betulaefolia and will aid in decoding the precise roles of AMTs in P. betulaefolia physiology.
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Compuestos de Amonio/metabolismo , Proteínas de Transporte de Catión/genética , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Proteínas de Plantas/genética , Pyrus/genética , Transcripción Genética , Secuencia de Aminoácidos , Secuencia de Bases , Proteínas de Transporte de Catión/química , Proteínas de Transporte de Catión/metabolismo , ADN Complementario/genética , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Cinética , Nitrógeno/farmacología , Especificidad de Órganos/efectos de los fármacos , Especificidad de Órganos/genética , Fotoperiodo , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Pyrus/efectos de los fármacos , Saccharomyces cerevisiae/metabolismo , Alineación de Secuencia , Transcripción Genética/efectos de los fármacosRESUMEN
INTRODUCTION: Sexual curiosity and the quest for sexual excitement are the most frequent reasons for patients to introduce foreign bodies into the urethra or the bladder. Imagination and surgical skill are essential for urologists to retrieve such vesical foreign bodies. AIM: The aim of this study was to describe a novel method for retrieving vesical magnetic beads, which were inserted for autoeroticism by a male adolescent, with a self-made "magnetic sheath." METHODS: A 21-year-old young man inserted more than one hundred small magnetic beads into his urethra for sexual excitement, which lately caused symptoms of gross hematuria, frequent urination, and acute lower abdominal pain when walking or urinating. We invented a magnetic sheath by fixing a magnetic bead on the tip of an F9.5 ureteral access sheath to remove the foreign bodies in a minimally invasive way. MAIN OUTCOME MEASURE: The feasibility of using magnetic sheath to remove vesical foreign bodies; and operation duration. RESULTS: Under direct visualization of an F8/9.8 ureteroscope, the magnetic sheath could firmly attach to the magnetic bead inside the bladder and could easily pull out 5 to 15 beads each time. It took about 5 minutes to remove all of the 125 magnetic beads by utilizing our magnetic sheath. CONCLUSIONS: The self-made magnetic sheath can make the task of removal of magnetic foreign bodies easy to urologists, requiring less time and surgical skills. The new equipment provides a new method for urologists to deal with the challenging task of removing metal vesical foreign bodies which were self-inserted for masturbation.
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Cuerpos Extraños/complicaciones , Cuerpos Extraños/cirugía , Migración de Cuerpo Extraño/complicaciones , Prepucio/lesiones , Fenómenos Magnéticos , Imanes/efectos adversos , Uretra/lesiones , Enfermedades de la Vejiga Urinaria/etiología , Adolescente , Migración de Cuerpo Extraño/cirugía , Humanos , Masculino , Masturbación , Procedimientos Quirúrgicos Mínimamente Invasivos , Conducta Sexual , Resultado del Tratamiento , Enfermedades de la Vejiga Urinaria/cirugía , Adulto JovenRESUMEN
Coral associated bacterial community potentially has functions relating to coral health, nutrition and disease. Culture-free, 16S rRNA based techniques were used to compare the bacterial community of coral tissue, mucus and seawater around coral, and to investigate the relationship between the coral-associated bacterial communities and environmental variables. The diversity of coral associated bacterial communities was very high, and their composition different from seawater. Coral tissue and mucus had a coral associated bacterial community with higher abundances of Gammaproteobacteria. However, bacterial community in seawater had a higher abundance of Cyanobacteria. Different populations were also found in mucus and tissue from the same coral fragment, and the abundant bacterial species associated with coral tissue was very different from those found in coral mucus. The microbial diversity and OTUs of coral tissue were much higher than those of coral mucus. Bacterial communities of corals from more human activities site have higher diversity and evenness; and the structure of bacterial communities were significantly different from the corals collected from other sites. The composition of bacterial communities associated with same coral species varied with season's changes, geographic differences, and coastal pollution. Unique bacterial groups found in the coral samples from more human activities location were significant positively correlated to chemical oxygen demand. These coral specific bacteria lead to coral disease or adjust to form new function structure for the adaption of different surrounding needs further research.
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Antozoos/microbiología , Microbiota , Agua de Mar/microbiología , Animales , Bacterias/clasificación , Bacterias/genética , Bacterias/aislamiento & purificación , China , Arrecifes de Coral , Cianobacterias/clasificación , Cianobacterias/genética , Cianobacterias/aislamiento & purificación , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Ambiente , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética , ARN Ribosómico 16S/metabolismo , Análisis de Secuencia de ADNRESUMEN
A novel dimer of piceatannol glycoside, named rheumaustralin (1) was isolated from the underground parts of the ethnomedicinal plant Rheum austral (Polygonaceae) collected from Tibet together with 17 known compounds, including rheumin (2), 2,5-dimethyl-7-hydroxychromone (3), 2,5-dimethylchromone-7-O-ß-D-glucopyranoside (4), 7-hydroxy-2-(2'-hydroxypropyl)-5-methylchromone (5), torachrysone (6) torachrysone-8-O-ß-D-glucopyranoside (7), 4-(4'-hydroxyphenyl)-2-butanone-4'-O-ß-D-glucopyranoside (8), amabiloside (9), N-trans-feruloyl tyramine (10), chrysophanol (11), aloe-emodin (12), emodin (13), physcion (14), physcion-1-O-ß-D-glucopyranoside (15), emodin-8-O-ß-D-glucopyranoside (16), D-catechin (17) and gallic acid (18). Their structures were determined by combined spectroscopic methods and by comparison of their spectral data with those reported in literature. Compounds 1-10 were tested for their ability to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical.
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Antioxidantes/química , Antioxidantes/farmacología , Rheum/química , Estilbenos/química , Estilbenos/farmacología , Dimerización , Depuradores de Radicales Libres/química , Depuradores de Radicales Libres/farmacología , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Extractos Vegetales/química , Extractos Vegetales/farmacologíaRESUMEN
Two new resveratrol trimer derivatives, named rheumlhasol A (1) and rheumlhasol B (2) were isolated from the methanolic extract of roots of Rheum lhasaense A. J. Li et P. K. Hsiao together with four known resveratrol dimer derivatives, including maximol A (3), gnetin C (4), ε-viniferin (5), and pallidol (6). The structures were determined by combined spectroscopic methods and by comparison of their spectral data with those reported in the literature. All the compounds isolated from R. lhasaense were tested for their ability to scavenge1,1-diphenyl-2-picrylhydrazyl (DPPH) radical.
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Raíces de Plantas/química , Rheum/química , Estilbenos/química , Antioxidantes/química , Antioxidantes/farmacología , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Extractos Vegetales/química , Resveratrol , Estereoisomerismo , Estilbenos/aislamiento & purificaciónRESUMEN
We report the discovery and detailed investigation of superconductivity in Mo4Ga20As. Mo4Ga20As crystallizes in a space group ofI4/m(No. 87), with the lattice parametersa= 12.86352 Å andc= 5.30031 Å. The resistivity, magnetization, and specific heat data reveal Mo4Ga20As to be a type-II superconductor withTc= 5.6 K. The upper and lower critical fields are estimated to be 2.78 T and 22.0 mT, respectively. In addition, electron-phonon coupling in Mo4Ga20As is possibly stronger than the BCS weak-coupling limit. First-principles calculations suggest the Fermi level being dominated by the Mo-4dand Ga-4porbitals.
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Glioma is one of the most common malignancies of the nervous system. Long noncoding RNAs (lncRNAs) are regulators involved in the progression of tumors. The present study aimed to determine the role of lncRNA cancer susceptibility 19 (CASC19) in glioma and its underlying molecular mechanism. Reverse transcriptionquantitative PCR was performed to detect CASC19 and microRNA (miR)4543p expression in glioma and normal brain tissues. Rasrelated protein in brain 5A (RAB5A) expression in glioma cells was also analyzed via western blotting. The relationship between CASC19 expression, clinicopathological parameters and MRI characteristics in patients with glioma was analyzed. Cell Counting Kit8, BrdU, wound healing and Transwell assays were adopted to detect glioma cell proliferation, migration and invasion, respectively. The dualluciferase reporter gene and RNA immunoprecipitation experiments were conducted to verify the targeting relationship between CASC19 and miR4543p, and between miR4543p and RAB5A. The results revealed that CASC19 expression was significantly upregulated in glioma tissues and cell lines. CASC19 expression was also positively associated with tumor diameter and pathological grade. Additionally, its high expression was closely associated with tumor MRI signal heterogeneity and peritumoral edema. CASC19 upregulation promoted glioma cell proliferation and metastasis, while CASC19knockdown demonstrated the opposite effect. CASC19 sponged miR4543p, which indirectly increased RAB5A expression. The results demonstrated that the CASC19/miR4543p/RAB5A axis is involved in the promotion of glioma progression.
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Neoplasias Encefálicas/genética , Glioma/genética , MicroARNs/metabolismo , ARN Largo no Codificante/metabolismo , Proteínas de Unión al GTP rab5/genética , Adulto , Anciano , Anciano de 80 o más Años , Apoptosis/genética , Encéfalo/diagnóstico por imagen , Encéfalo/patología , Encéfalo/cirugía , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/cirugía , Línea Celular Tumoral , Proliferación Celular , Femenino , Regulación Neoplásica de la Expresión Génica , Glioma/diagnóstico , Glioma/cirugía , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Regulación hacia ArribaRESUMEN
Colorectal cancer (CRC) is a common malignancy with significant prevalence and mortality rates. Circular RNA FOXO3 (circFOXO3; hsa_circ_0006404) has been reported to be involved in cancer regulation; however, its role in CRC is yet to be fully elucidated. Therefore, the aim of the present study was to investigate the effect of circFOXO3 on CRC progression and identify its underlying mechanism. In the present study, the expression of circFOXO3 was investigated in CRC tissues and cells via reverse transcriptionquantitative PCR. A Cell Counting Kit8 and colony formation assays were used to assess cell proliferation. The cell migratory and invasive abilities were detected using the Transwell migration and invasion assays. The luciferase assay and RNA pulldown assay were conducted to verify the relationship of circFOXO3, microRNA (miR)543 and Large tumor suppressor kinase 1 (LATS1). The results demonstrated that circFOXO3 expression was downregulated in CRC tissues and cells, and was associated with poor overall survival of patients with CRC. Moreover, circFOXO3 was associated with tumor size, distant metastasis, differentiation, lymph node metastasis and TMN stages of patients with CRC. circFOXO3 overexpression suppressed CRC cell proliferation, migration and invasion. Luciferase assay and RNA pulldown assay results indicated that circFOXO3 functioned as a sponge for miR543. In addition, circFOXO3 increased the expression of LATS1 via sponging miR543, thus inhibiting CRC cell aggressive features. Collectively, the present results suggested that circFOXO3 inhibited CRC metastasis and progression via elevated LATS1 expression by sponging miR543. Therefore, circFOXO3 may be a promising target for CRC therapy.
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Neoplasias Colorrectales/metabolismo , Proteína Forkhead Box O3/metabolismo , MicroARNs/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Adulto , Anciano , Línea Celular Tumoral , Movimiento Celular , Proliferación Celular , Regulación hacia Abajo , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
A cognitive magnetic resonance imaging (MRI)-targeted prostate biopsy conducted by an experienced clinician enhances the detection rate of (high-grade) prostate cancer; however, this method is less successful in the hands of inexperienced surgeons. Therefore, an alternative method of conducting a cognitive MRI-targeted biopsy that can be successfully performed by the inexperienced clinicians should be developed. Ninety-six males suspected of prostate cancer were analyzed using systematic biopsy and cognitive MRI-targeted biopsy based on our novel three-dimensional matrix positioning method. Typically, the core principle of the latter procedure was to put the MRI and ultrasound images into the same virtual coordinate system. Afterward, the targeted biopsy was transformed to target a coordinate for the suspected lesion in the MRI. Subsequently, patients were assessed for the presence/absence of prostate cancer or high-grade prostate cancer. According to our results, the overall detection rate of prostate cancer was 70.8% (68/96), and the detection rate of high-grade prostate cancer was 56.3% (54/96). Specifically, the detection rate of prostate cancer by systematic biopsy was 54.2% (52/96) and that by targeted biopsy was 59.4% (57/96; P = 0.560). Clearly, the combined application of targeted biopsy could remarkably increase the detection rates of prostate cancer (P = 0.025) and high-grade prostate cancer (P = 0.009). Taken together, the findings of this study suggest that the combination of systematic biopsy with our three-dimensional matrix positioning-driven cognitive-targeted biopsy is superior to systematic biopsy in detecting prostate cancer and high-grade prostate cancer.
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Biopsia con Aguja Gruesa/métodos , Biopsia Guiada por Imagen/métodos , Imagen por Resonancia Magnética , Próstata/patología , Neoplasias de la Próstata/patología , Anciano , Humanos , Imagenología Tridimensional , Masculino , Persona de Mediana Edad , Clasificación del Tumor , Perineo , Antígeno Prostático Específico/sangre , Neoplasias de la Próstata/sangre , Programas Informáticos , UltrasonografíaRESUMEN
Two new phenylpropanoids (1-2), together with two known lignans (3-4), were isolated from whole herb of Swertia atroviolacea. The structures of the new metabolites were established on the basis of detailed spectroscopic analysis. Compounds 1 and 2 were evaluated for their anti-5α-reductase activity. The results revealed that 1 and 2 showed weak activity with reductase inhibitions of 45.6 ± 2.8% and 38.4 ± 2.5%, respectively.
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Inhibidores de 5-alfa-Reductasa/aislamiento & purificación , Fenilpropionatos/aislamiento & purificación , Swertia/química , Inhibidores de 5-alfa-Reductasa/farmacología , Lignanos/aislamiento & purificación , Fenilpropionatos/química , Fenilpropionatos/farmacologíaRESUMEN
OBJECTIVE: In this study, the clinical effect of the Nd: YAG laser embolysis on branch retinal artery occlusion was evaluated. METHODS: A fundus contact lens was used to focus the Nd: YAG laser on the embolus within the retinal arteriole. 0.8 to 0.9 mJ of laser pulse was delivered directly to the embolus. Fundus photographs were taken before and immediately after the treatment. Follow-up time ranged from 2 weeks to 4 months, average about 7 weeks. Seven cases received the treatment, including 5 male and 2 female patients. Age of those cases ranged from 49 to 73, average 61 years. The duration of the disease ranged from 1 to 60 days, average 13 days. RESULTS: Before treatment, visual acuity ranged from 0.06 to 0.8. Embolus can be detected in all the 7 cases. After treatment, all the cases showed immediate recovery of blood flow in the occluded arteriole both in fundus examination and fundus fluorescein angiography. The vision in the patients was improved in the follow-up. Visual acuity ranged from 0.2 approximately 1.0. In most cases, visual field defect still existed in the ischemic area. Complications included local retinal hemorrhages in some cases and vitreous hemorrhage, but those hemorrhages were gradually absorbed and did not affect the final visual prognosis. CONCLUSIONS: Nd: YAG laser embolysis for branch retinal artery occlusion is an effective treatment and the treatment is relatively safe.
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Terapia por Láser/métodos , Oclusión de la Arteria Retiniana/cirugía , Anciano , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Resultado del Tratamiento , Agudeza VisualRESUMEN
Two new xanthones (1-2), together with four known ones (3-6), were isolated from whole herb of Swertia elata. Their structures were elucidated by spectroscopic methods including extensive 1D- and 2D-NMR techniques. Their anti-tobacco mosaic virus (anti-TMV) activity test revealed that 1-6 showed weak anti-TMV activities with inhibition rate in the range of 15.2-28.8% at the concentration of 20 µM.
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Swertia/química , Virus del Mosaico del Tabaco/efectos de los fármacos , Xantonas/aislamiento & purificación , Antivirales/química , Antivirales/aislamiento & purificación , Antivirales/farmacología , Espectroscopía de Resonancia Magnética , Estructura Molecular , Xantonas/química , Xantonas/farmacologíaRESUMEN
The pervasive pattern of aggregated tree distributions in natural communities is commonly explained by the joint effect of two clustering processes: environmental filtering and dispersal limitation, yet little consensus remains on the relative importance of the two clustering processes on tree aggregations. Different life stages of examined species were thought to be one possible explanation of this disagreement, because the effect of environmental filtering and dispersal limitation are expected to increase and decrease with tree life stages, respectively. However, few studies have explicitly tested these expectations. In this study, we evaluated these expectations by three different methods (species-habitat association test based on Poisson Clustering model and spatial point pattern analyses based on Heterogeneous Poisson model and the jointly modeling approach) using 36 species in a 20-ha subtropical forest plot. Our results showed that the percentage of species with significant habitat association increased with life stages, and there were fewer species affected by dispersal limitation in later life stages compared with those in earlier stages. Percentage of variance explained by the environmental filtering and dispersal limitation also increases and decreases with life stages. These results provided a promising alternative explanation on the existing mixed results about the relative importance of the two clustering processes. These findings also highlighted the importance of plant life stages for fully understanding species distributions and species coexistence.
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Ambiente , Modelos Teóricos , Dispersión de las Plantas/fisiología , Árboles/fisiología , Análisis por Conglomerados , Ecosistema , Bosques , Densidad de Población , Especificidad de la Especie , Árboles/clasificación , Clima TropicalRESUMEN
BACKGROUND: Copernicus optical coherence tomography (SOCT) is a new, ultra high-speed and high-resolution instrument available for clinical evaluation of optic nerve. The purpose of the study was to compare the agreements between SOCT and Heidelberg retinal tomography (HRT). METHODS: A total of 44 healthy normal volunteers were recruited in this study. One eye in each subject was selected randomly. Agreement between SOCT and HRT-3 in measuring optic disc area was assessed using Bland-Altman plots. Relationships between measurements of optic nerve head parameter obtained by SOCT and HRT-3 were assessed by Pearson correlation. RESULTS: There was no significant difference in the average cup area (0.306 vs. 0.355 mm, P = 0.766), cup volume (0.158 vs. 0.130 mm, P = 0.106) and cup/disc ration (0.394 vs. 0.349 mm, P = 0.576) measured by the two instruments. However, other optic disc parameters from SOCT were significantly lower compared with HRT-3. The Bland-Altman plot revealed good agreement of cup area and cup volume measured by SOCT and HRT-3. Bad agreement of disc area, rim area, rim volume and cup/disc ratio were found between SOCT and HRT-3. The highest correlations between the two instruments were observed for cup area (r(2) = 0.783, P = 0.000) and cup/disc ratio (r(2) = 0.669, P = 0.000), whereas the lowest correlation was observed for disc area (r(2) = 0.100, P = 0.037), rim area (r(2) = 0.275, P = 0.000), cup volume (r(2) = 0.005, P = 0.391) and rim volume (r(2) = 0.021, P = 0.346). CONCLUSIONS: There were poor agreements between SOCT and HRT-3 for measurement of optic nerve parameters except cup area and cup volume. Measurement results of the two instruments are not interchangeable.
Asunto(s)
Disco Óptico/patología , Retina/patología , Tomografía de Coherencia Óptica/métodos , Tomografía/métodos , Adolescente , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
By using geostatistical methods, this paper studied the spatial heterogeneity and distribution patterns of soil pH, total carbon, total nitrogen, and total phosphorus in an evergreen broad-leaved forest in Tiantong of Zhejiang Province, and the effects of terrain factors (elevation, convexity, and slope) on the soil properties were quantified based on RDA ordination and partial regression analysis. The coefficient of variation for the soil pH, total carbon, total nitrogen, and total phosphorus was 5.18%, 42.98%, 36.55%, and 46.27%, respectively, and the spatial dependence of the soil properties was at a scale of 81.6-54.5 m. The soil pH, total carbon, and total nitrogen had moderate spatial autocorrelation, while the soil total phosphorus had a strong spatial autocorrelation. The soil pH, total carbon, and total nitrogen showed scattered spatial distribution, while the soil total phosphorus presented banded type. Among the terrain factors, convexity had the strongest effects on the soil pH and total phosphorus, both of which had negative correlation with convexity, and the convexity could explain 21.24% and 14.62% of the spatial variability of soil pH and total phosphorus, respectively. Elevation had the most powerful effects on the soil total carbon and total nitrogen, both of which had positive correlation with elevation, and the elevation could explain 10.54% and 10.60% of the spatial variability of soil total carbon and total nitrogen, respectively. There existed differences in the effects of different terrain factors on the spatial variability of the soil properties, which was related to the effects of terrain factors on the distribution of acidic rainfall in the region and on the local soil moisture content and air temperature.