RESUMEN
A major goal of modern neuroscience is to understand the functions of the varied neuronal types that comprise the mammalian brain. Toward this end, some types of neurons can be targeted and manipulated with enhancer-bearing AAV vectors. These vectors hold great promise to advance basic and translational neuroscience, but to realize this potential, their selectivity must be characterized. In this study, we investigated the selectivity of AAV vectors carrying an enhancer of the murine Dlx5 and Dlx6 genes. Vectors were injected into the visual cortex of two macaque monkeys, the frontal cortex of two others, and the somatosensory/motor cortex of three rats. Post-mortem immunostaining revealed that parvalbumin-expressing neurons were transduced efficiently in all cases but calretinin-expressing neurons were not. We speculate that this specificity is a consequence of differential activity of this DLX5/6 enhancer in adult neurons of different developmental lineages.
RESUMEN
The present study investigated the visual scanning pattern of children with typical development in three different age groups(4-6,6-8,8-10 years old). We used a data set from one related research, which included images with different low-level features: Green and Normal. This study analyzed age-associated inter-individual differences and was intended to show that graph profiling combined with a fixation time approach could help us to better understand the developmental visual pattern. Thus, degree centrality as one of the graph theory measures was implied to analyze gaze distribution. We explored the influence of bottom-up features, comparing the first 2 s (early phase) with the interval from 4 to 6 s (late phase) of scene exploration during age development. Our results indicated that degree centrality and fixation time increased with age. Furthermore, it was found that the effects of saliency are short-lived but significant. Moreover, we found that Green images during the early phase play an important role in visual anchoring, and the children's performance was significantly different between 4-6 y and 6-8y-group. This comparative study underscores the ability of degree centrality as a developing innovative measure to perform eye-tracking data analyses.