Nonenzymatic Target-Driven DNA Nanomachine for Monitoring Malathion Contamination in Living Cells and Bioaccumulation in Foods.

Intensive applications of toxic malathion pesticides bring a vital threat to the environment and health. Hence, a credible and sensitive strategy is urgently needed for the respective detection of malathion. In this work, an aptamer-based nonenzymatic autonomous DNA walking machine was fabricated for monitoring trace malathion contamination in cells and foods. Along with the machine walking driven by malathion-triggered reaction entropy, multiple fluorescent signal outputs were thermodynamically generated for signal amplification. The proposed stable DNA nanomachine achieved satisfactory results with a detection limit of 81.9 pg L-1 for testing malathion, which could be applied to actual samples including apple juice, paddy water, and paddy soil. Furthermore, the high stability, sensitivity, and biocompatibility of the nanomachine enabled monitoring of the malathion contamination in living cells and bioaccumulation in lettuce without additional purification. Consequently, with these excellent performances, it is strongly anticipated that the DNA walking machine has tremendous potential to be extended to general platforms against pesticides to avoid malathion-contaminated agricultural production for environmental safety and human health.

Highly sensitive fluorescent quantification of acid phosphatase activity and its inhibitor pesticide Dufulin by a functional metal-organic framework nanosensor for environment assessment and food safety.

Developing a rapid and accurate strategy of sensing Dufulin is a vital challenge for risk assessment and food crops along with its spreading usage. Herein a dye-encapsulated azoterephthalate metal-organic framework (MOF)-based fluorescent sensing system was designed for Dufulin analysis by acid phosphatase (ACP) enzyme-controlled collapse of MOF framework and subsequent release of the encapsulated dye. The fluorescence intensity of the DMOF/AAP/ACP system was negatively related to the dosage of Dufulin (0-5 µg mL-1) with detection limit of 2.96 ng mL-1. The sensing system able to rapidly and sensitively sense the activity of ACP and Dufulin, and was also applicable for assessment of the real samples including paddy water and soil, polished rice and cucumber. Accordingly, this study illustrated the feasibility and the potential of MOF-derived nanosensors for improving pesticide analysis and opening up the design of the enzyme-based probes for pesticide sensing in environmental assessment and food safety.

A cerium oxide@metal-organic framework nanoenzyme as a tandem catalyst for enhanced photodynamic therapy.

An O2 self-evolving core-shell theranostic nanohybrid was developed by encapsulating a nanoenzyme cerium oxide (CeOx) in a metal-organic framework (MOF). The hybrid reveals a 9-fold higher apoptotic percentage than bare CeOx in a harsh hypoxic microenvironment through tandem homogenous catalysis. Simultaneously, the oxygen-promoted therapeutic efficiency was self-monitored by the hybrid with caspase-3 activation, paving the way for MOF-functionalized nanoenzymes in theranostics.

Developing a Novel Nanoscale Porphyrinic Metal-Organic Framework: A Bifunctional Platform with Sensitive Fluorescent Detection and Elimination of Nitenpyram in Agricultural Environment.

Developing a rapid sensing platform with effective pesticide degradation capabilities integrated into a single structure and realistic application is an imminent challenge to ensure sustainable agriculture and food safety. Here, we described establishment of a bifunctional nanoscale porphyrinic metal-organic framework (MOF) probe serving as a sensor for detection of trace nitenpyram and as a photocatalyst to facilitate the pesticide degradation. Based on the signal turned "on-off", the strong fluorescence of the probe was quenched by the target, leading to the sensing range from 0.05 to 10.0 µg mL-1 and a detection limit of 0.03 µg mL-1. Given the versatile design by which the porphyrin photosensitizers were isolated subtly in the MOF to avoid self-quenching, the probe was endowed with sustainable and efficient pesticide photodegradation activity with a degradation rate of ∼95% for nitenpyram. Our work represents powerful all-in-one MOF-derived materials jointly for sensing and degrading pesticide residues in agricultural soils and other pesticide-contaminated environments.