Aberration correction in abdominal histotripsy.

In transabdominal histotripsy, ultrasound pulses are focused on the body to noninvasively destroy soft tissues via cavitation. However, the ability to focus is limited by phase aberration, or decorrelation of the ultrasound pulses due to spatial variation in the speed of sound throughout heterogeneous tissue. Phase aberration shifts, broadens, and weakens the focus, thereby reducing the safety and efficacy of histotripsy therapy. This paper reviews and discusses aberration effects in histotripsy and in related therapeutic ultrasound techniques (e.g., high intensity focused ultrasound), with an emphasis on aberration by soft tissues. Methods for aberration correction are reviewed and can be classified into two groups: model-based methods, which use segmented images of the tissue as input to an acoustic propagation model to predict and compensate phase differences, and signal-based methods, which use a receive-capable therapy array to detect phase differences by sensing acoustic signals backpropagating from the focus. The relative advantages and disadvantages of both groups of methods are discussed. Importantly, model-based methods can correct focal shift, while signal-based methods can restore substantial focal pressure, suggesting that both methods should be combined in a 2-step approach. Aberration correction will be critical to improving histotripsy treatments and expanding the histotripsy treatment envelope to enable non-invasive, non-thermal histotripsy therapy for more patients.

In Vivo Cavitation-Based Aberration Correction of Histotripsy in Porcine Liver.

Histotripsy is a noninvasive ablation technique that focuses ultrasound pulses into the body to destroy tissues via cavitation. Heterogeneous acoustic paths through tissue introduce phase errors that distort and weaken the focus, requiring additional power output from the histotripsy transducer to perform therapy. This effect, termed phase aberration, limits the safety and efficacy of histotripsy ablation. It has been shown in vitro that the phase errors from aberration can be corrected by receiving the acoustic signals emitted by cavitation. For transabdominal histotripsy in vivo, however, cavitation-based aberration correction (AC) is complicated by acoustic signal clutter and respiratory motion. This study develops a method that enables robust, effective cavitation-based AC in vivo and evaluates its efficacy in the swine liver. The method begins with a high-speed pulsing procedure to minimize the effects of respiratory motion. Then, an optimal phase correction is obtained in the presence of acoustic clutter by filtering with the singular value decomposition (SVD). This AC method reduced the power required to generate cavitation in the liver by 26% on average (range: 0%-52%) and required ~2 s for signal acquisition and processing per focus location. These results suggest that the cavitation-based method could enable fast and effective AC for transabdominal histotripsy.

Soft Tissue Aberration Correction for Histotripsy Using Acoustic Emissions From Cavitation Cloud Nucleation and Collapse.

OBJECTIVE: Phase aberration from soft tissue limits the efficacy of histotripsy, a therapeutic ultrasound technique based on acoustic cavitation. Previous work has shown that the acoustic emissions from cavitation can serve as "point sources" for aberration correction (AC). This study compared the efficacy of soft tissue AC for histotripsy using acoustic cavitation emissions (ACE) from bubble cloud nucleation and collapse. METHODS: A 750-kHz, receive-capable histotripsy array was pulsed to generate cavitation in ex vivo porcine liver through an intervening abdominal wall. Received ACE signals were used to determine the arrival time differences to the focus and compute corrective delays. Corrections from single pulses and from the median of multiple pulses were tested. DISCUSSION: On average, ACE AC obtained 96% ± 3% of the pressure amplitude obtained by hydrophone-based correction (compared with 71% ± 5% without AC). Both nucleation- and collapse-based corrections obtained >96% of the hydrophone-corrected pressure when using medians of ≥10 pulses. When using single-pulse corrections, nucleation obtained a range of 49%-99% of the hydrophone-corrected pressure, while collapse obtained 95%-99%. CONCLUSION: The results suggest that (i) ACE AC can recover nearly all pressure amplitude lost owing to soft tissue aberration and that (ii) the collapse signal permits robust AC using a small number of pulses.

Effects of phase aberration on transabdominal focusing for a large aperture, lowf-number histotripsy transducer.

Objective. Soft tissue phase aberration may be particularly severe for histotripsy due to large aperture and lowf-number transducer geometries. This study investigated how phase aberration from human abdominal tissue affects focusing of a large, strongly curved histotripsy transducer.Approach.A computational model (k-Wave) was experimentally validated withex vivoporcine abdominal tissue and used to simulate focusing a histotripsy transducer (radius: 14.2 cm,f-number: 0.62, central frequencyfc: 750 kHz) through the human abdomen. Abdominal computed tomography images from 10 human subjects were segmented to create three-dimensional acoustic property maps. Simulations were performed focusing at 3 target locations in the liver of each subject with ideal phase correction, without phase correction, and after separately matching the sound speed of water and fat to non-fat soft tissue.Main results.Experimental validation in porcine abdominal tissue showed that simulated and measured arrival time differences agreed well (average error, ∼0.10 acoustic cycles atfc). In simulations with human tissue, aberration created arrival time differences of 0.65µs (∼0.5 cycles) at the target and shifted the focus from the target by 6.8 mm (6.4 mm pre-focally along depth direction), on average. Ideal phase correction increased maximum pressure amplitude by 95%, on average. Matching the sound speed of water and fat to non-fat soft tissue decreased the average pre-focal shift by 3.6 and 0.5 mm and increased pressure amplitude by 2% and 69%, respectively.Significance.Soft tissue phase aberration of large aperture, lowf-number histotripsy transducers is substantial despite low therapeutic frequencies. Phase correction could potentially recover substantial pressure amplitude for transabdominal histotripsy. Additionally, different heterogeneity sources distinctly affect focusing quality. The water path strongly affects the focal shift, while irregular tissue boundaries (e.g. fat) dominate pressure loss.