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1.
J Dairy Sci ; 101(6): 4830-4841, 2018 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-29573807

RESUMEN

In a preliminary open-label trial by our group, Bifidobacterium bifidum YIT 10347 (YIT10347) relieved gastric symptoms in patients with functional gastrointestinal disorders. Hence, in this study, we investigated the effects of YIT10347 on gastrointestinal symptoms in healthy adults. In this prospective double-blind, randomized, placebo-controlled trial (UMIN000024654), 100 healthy Japanese adults were randomly assigned to a YIT10347 group or placebo group and consumed 100 mL of YIT10347-fermented milk or placebo fermented milk, respectively, every day for 4 wk. Gastrointestinal symptoms were evaluated by using the modified Frequency Scale for Symptoms of Gastroesophageal Reflux Disease (m-FSSG) and Gastrointestinal Symptom Rating Scale (GSRS) as primary endpoints. Mental symptoms, quality of life, salivary stress markers, and gastric emptying were evaluated as secondary endpoints. Effectiveness and safety were analyzed in a per-protocol set (YIT10347 group, n = 39; placebo group, n = 40) and full analysis set (YIT10347 group, n = 50; placebo group, n = 50), respectively. In the m-FSSG evaluation, the YIT10347 group had a significantly higher relief rate of postprandial discomfort and greater changes in postprandial epigastric pain score from baseline than the placebo group. In the GSRS evaluation, the YIT10347 group had significantly higher relief rates of overall gastrointestinal symptoms, upper gastrointestinal symptoms, flatus, and diarrhea than the placebo group. We detected no significant differences in scores or relief rates of mental symptoms and quality of life, a salivary stress marker, or gastric emptying between the 2 groups. No severe adverse events associated with test beverage consumption were observed in either group. These findings suggest that daily consumption of YIT10347-fermented milk exerts beneficial effects on gastrointestinal discomfort and symptoms such as postprandial discomfort and epigastric pain in healthy adults.


Asunto(s)
Bifidobacterium bifidum/metabolismo , Productos Lácteos Cultivados/microbiología , Enfermedades Gastrointestinales/tratamiento farmacológico , Probióticos/administración & dosificación , Adulto , Animales , Productos Lácteos Cultivados/análisis , Método Doble Ciego , Femenino , Fermentación , Vaciamiento Gástrico/efectos de los fármacos , Enfermedades Gastrointestinales/dietoterapia , Enfermedades Gastrointestinales/fisiopatología , Humanos , Japón , Masculino , Persona de Mediana Edad , Leche/metabolismo , Leche/microbiología , Estudios Prospectivos , Calidad de Vida , Adulto Joven
2.
Int J Cosmet Sci ; 39(1): 49-55, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27261166

RESUMEN

OBJECTIVE: A higher trans-epidermal water loss (TEWL) occurs in rough skin, in elder skin and also in atopic dermatitis. An impaired skin barrier function is considered to be caused by an incomplete construction of the intercellular lamellar structure due to the quantitative reduction of ceramides. Since these symptoms coexist with oxidative stress, we hypothesized that impairment of the skin barrier function is accelerated by oxidative stress. Thus, the purpose of this study was to clarify the effect of oxidative stress on ceramide synthesis and to characterize whether antioxidants can improve skin barrier function. 3-O-Laurylglyceryl ascorbate (VC-3LG), which is a newly amphipathic derivative of ascorbic acid, was evaluated as a candidate antioxidant. METHODS: We characterized the mRNA expression levels of serine palmitoyltransferase (SPT) in normal human epidermal keratinocytes (NHEKs) treated with H2 O2 using real-time PCR analysis. In order to evaluate the effect of VC-3LG on skin barrier function, we used several assays with reconstructed human epidermis equivalents (RHEEs). RESULTS: Ceramide synthesis was down-regulated in NHEKs by oxidative stress. Treatment with VC-3LG abrogated the down-regulation of SPT mRNA in NHEKs caused by oxidative stress, and stimulated SPT mRNA expression levels. In experiments characterizing the antioxidative properties of VC-3LG, VC-3LG reduced oxidative stress in NHEKs by up-regulating catalase mRNA expression. In addition, VC-3LG stimulated the skin barrier function in RHEEs, which had lower TEWL values compared with untreated RHEEs. Furthermore, VC-3LG increased the quantity of ceramide in RHEEs. CONCLUSION: Taken together, we conclude that VC-3LG reinforces the skin barrier function due to its reduction of oxidative stress and its promotion of ceramide synthesis.


Asunto(s)
Ácido Ascórbico/análogos & derivados , Ceramidas/biosíntesis , Estrés Oxidativo/efectos de los fármacos , Absorción Cutánea/efectos de los fármacos , Antioxidantes/farmacología , Ácido Ascórbico/farmacología , Células Cultivadas , Humanos , PPAR alfa/genética , ARN Mensajero/genética , Serina C-Palmitoiltransferasa/genética , Regulación hacia Arriba
3.
J Periodontal Res ; 49(5): 603-8, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24206088

RESUMEN

BACKGROUND AND OBJECTIVE: Antibiotics at below minimal inhibitory concentrations (MICs) may induce various biological responses in bacteria. In this study, we hypothesized that subinhibitory concentrations (subICs) of tetracycline and doxycycline induce the shedding of lipopolysaccharide (LPS) by Porphyromonas gingivalis and, as a consequence, may contribute to enhancing the host inflammatory response associated with periodontitis. MATERIAL AND METHODS: A polymyxin-based enzyme-linked immunosorbent assay was used to quantify LPS shedding by P. gingivalis grown in the presence of subICs of tetracycline and doxycycline. A macrophage model was used to show that tetracycline- and doxycycline-mediated LPS shedding by P. gingivalis can induce cytokine secretion. The secretion of interleukin (IL)-1ß, IL-8, and tumor necrosis factor-α was quantified by enzyme-linked immunosorbent assay. RESULTS: LPS was shed spontaneously in a time-dependent way by P. gingivalis during growth. LPS shedding was significantly increased by growth in the presence of subICs of tetracycline and doxycycline corresponding to 1/20 of their MICs (0.025 µg/mL for tetracycline and 0.0125 µg/mL for doxycycline). This shedding was not associated with an increased rate of bacterial cell lysis. Stimulating macrophages with a P. gingivalis culture supernatant induced the secretion of IL-1ß, IL-8 and tumor necrosis factor-α when the bacteria were grown in the presence of 1/20 MIC of the antibiotics. CONCLUSION: Our study showed that growing P. gingivalis in the presence of subICs of either tetracycline or doxycycline induces LPS shedding. Shed LPS may in turn increase cytokine secretion in a macrophage model.


Asunto(s)
Antibacterianos/administración & dosificación , Doxiciclina/administración & dosificación , Interacciones Huésped-Patógeno/inmunología , Lipopolisacáridos/inmunología , Porphyromonas gingivalis/efectos de los fármacos , Tetraciclina/administración & dosificación , Anticuerpos Antibacterianos/inmunología , Técnicas Bacteriológicas , Técnicas de Cultivo de Célula , Humanos , Interleucina-1beta/efectos de los fármacos , Interleucina-8/efectos de los fármacos , Lipopolisacáridos/análisis , Macrófagos/efectos de los fármacos , Macrófagos/inmunología , Pruebas de Sensibilidad Microbiana , Porphyromonas gingivalis/inmunología , Factores de Tiempo , Factor de Necrosis Tumoral alfa/efectos de los fármacos , Células U937
4.
Indoor Air ; 24(4): 425-37, 2014 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-24354902

RESUMEN

UNLABELLED: Thermal conditions and indoor concentrations of aldehydes, volatile organic compounds (VOCs), and NO2 were investigated in 19 occupied temporary houses in 15 temporary housing estates constructed in Minamisoma City, Fukushima, Japan. The data were collected in winter, spring, and summer in January to July 2012. Thermal conditions in temporary log houses in the summer were more comfortable than those in pre-fabricated houses. In the winter, the indoor temperature was uncomfortably low in all of the houses, particularly the temporary log houses. Indoor air concentrations for most aldehydes and VOCs were much lower than the indoor guidelines, except for those of p-dichlorobenzene, acetaldehyde, and total VOCs. The indoor p-dichlorobenzene concentrations exceeded the guideline (240 µg/m(3)) in 18% of the temporary houses, and the 10(-3) cancer risk level (91 µg/m(3)) was exceeded in winter in 21% due to use of moth repellents by the occupants. Indoor acetaldehyde concentrations exceeded the guideline (48 µg/m(3) ) in about half of the temporary houses, likely originating from the wooden building materials. Indoor NO2 concentrations in the temporary houses were significantly higher in houses where combustion heating appliances were used (0.17 ± 0.11 ppm) than in those where they were not used (0.0094 ± 0.0065 ppm). PRACTICAL IMPLICATIONS: In the winter, log-house-type temporary houses are comfortable in terms of humidity, dew condensation, and fungi based on the results of questionnaires and measurements, whereas pre-fabricated temporary houses are more comfortable in terms of temperature. In the summer, log-house-type temporary houses are comfortable in terms of temperature and humidity. More comfortable temporary housing in terms of temperature and humidity year-round is needed. Indoor air concentrations of p-dichlorobenzene and NO2 were quite high in some temporary houses due to occupants' activities, such as use of moth repellents and combustion heating appliances. The government should provide recommendations for safe use of temporary houses by occupants.


Asunto(s)
Contaminantes Atmosféricos/análisis , Contaminación del Aire Interior/análisis , Exposición a Riesgos Ambientales/análisis , Monitoreo del Ambiente/métodos , Dióxido de Nitrógeno/análisis , Compuestos Orgánicos Volátiles/análisis , Distribución de Chi-Cuadrado , Terremotos , Calefacción , Vivienda , Humanos , Humedad , Japón , Estaciones del Año , Encuestas y Cuestionarios , Temperatura
5.
Int J Dent Hyg ; 12(2): 145-51, 2014 May.
Artículo en Inglés | MEDLINE | ID: mdl-23890391

RESUMEN

AIM: The objective of our study was to investigate the relationship between halitosis and oral bacteria in tongue coating (TC) and saliva samples from patients with halitosis, and to evaluate the effect of tongue cleaning on halitosis. METHODS: Ninety-four participants complaining of oral malodour were included in the study. Organoleptic (OR) values, volatile sulphur compound (VSC) concentrations determined by gas chromatography and TC scores were used as clinical parameters of halitosis. Quantitative real-time polymerase chain reactions were used to determine the numbers of periodontal disease-associated oral bacteria. RESULTS: There was a significant correlation between TC scores and OR values, methylmercaptan (CH3 SH) concentrations and VSC concentrations (Spearman's rank-correlation coefficient test, P < 0.01). There was also a positive correlation between the clinical parameters of halitosis and total bacterial numbers and Prevotella intermedia, Fusobacterium nucleatum and Campylobacter rectus concentrations in the TC samples. However, there was no similar correlation with respect to the saliva samples. The participants were sub-divided into two groups based on whether they had the habit of tongue cleaning or not. The participants with the habit of tongue cleaning had significantly lower OR scores, VSC concentrations and P. intermedia, F. nucleatum and C. rectus levels than the other participants (Mann-Whitney U-test, P < 0.05). CONCLUSION: These results suggested that periodontal disease-associated oral bacteria in TCs are closely related to halitosis and that tongue cleaning may be an effective method for improving halitosis.


Asunto(s)
Bacterias/clasificación , Halitosis/microbiología , Periodontitis/microbiología , Lengua/microbiología , Carga Bacteriana , Campylobacter rectus/aislamiento & purificación , Cromatografía de Gases , Femenino , Fusobacterium nucleatum/aislamiento & purificación , Humanos , Masculino , Persona de Mediana Edad , Higiene Bucal/métodos , Índice Periodontal , Bolsa Periodontal/clasificación , Bolsa Periodontal/microbiología , Porphyromonas gingivalis/aislamiento & purificación , Prevotella intermedia/aislamiento & purificación , Saliva/microbiología , Compuestos de Sulfhidrilo/análisis , Compuestos de Azufre/análisis , Lengua/patología , Compuestos Orgánicos Volátiles/análisis
6.
J Maxillofac Oral Surg ; 23(2): 290-293, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38601256

RESUMEN

Treatment of edentulous and atrophic mandibular fractures is extremely difficult. Generally, mandibular fractures are repaired and fixed as internal fixation using a reconstruction plate or miniplates with intra- or extraoral approach. Few cases in which external fixation including a transmucosal fixation was performed have also been reported. We report a case of atrophic and edentulous mandibular fracture which was healed by the fixation using dental implants and implant-supported bridge.

8.
Cells Tissues Organs ; 196(5): 411-9, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22653431

RESUMEN

Amelogenins are the major constituent of developing extracellular enamel matrix proteins and are understood to have an exclusively epithelial origin. Recent studies have demonstrated that amelogenins can be detected in other tissues, including bone marrow mesenchymal stem cells (MSCs), but the role of amelogenins in MSCs remains unclear. The purpose of this study was to examine the effect of recombinant human full-length amelogenin (rh174) on the osteogenic differentiation of cultured human MSCs. MSCs isolated from human bone marrow were cultured in osteoblastic differentiation medium with 0, 10 or 100 ng/ml rh174. The mRNA levels of bone markers were examined by real-time PCR analysis. Alkaline phosphatase (ALP) activity and calcium concentration were determined. Mineralization was evaluated by alizarin red staining. The mRNA levels of ALP, type I collagen, osteopontin and bone sialoprotein in the MSCs treated with rh174 became significantly higher than those in non-treated controls. Treatment of MSCs with rh174 also enhanced ALP activity and calcium concentration, resulting in enhanced mineralization, as denoted by high intensity of alizarin red staining. In conclusion, the present study showed that rh174 enhances the mineralization accompanied by the upregulation of bone markers in human bone marrow MSCs during osteogenic differentiation, suggesting a certain role of amelogenin in the modulation of osteogenic differentiation of MSCs.


Asunto(s)
Amelogenina/farmacología , Células de la Médula Ósea/citología , Células Madre Mesenquimatosas/citología , Osteogénesis/efectos de los fármacos , Diferenciación Celular/efectos de los fármacos , Células Cultivadas , Humanos , Células Madre Mesenquimatosas/efectos de los fármacos , Reacción en Cadena en Tiempo Real de la Polimerasa
13.
Cell Biochem Biophys ; 79(2): 321-336, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33559812

RESUMEN

Mesenchymal stem cell (MSC) transplantation is an effective periodontal regenerative therapy. MSCs are multipotent, have self-renewal ability, and can differentiate into periodontal cells. However, senescence is inevitable for MSCs. In vitro, cell senescence can be induced by long-term culture with/without cell passage. However, the regulatory mechanism of MSC senescence remains unclear. Undifferentiated MSC-specific transcription factors can regulate MSC function. Herein, we identified the regulatory transcription factors involved in MSC senescence and elucidated their mechanisms of action. We cultured human MSCs (hMSCs) with repetitive cell passages to induce cell senescence and evaluated the mRNA and protein expression of cell senescence-related genes. Additionally, we silenced the cell senescence-induced transcription factors, GATA binding protein 6 (GATA6) and SRY-box 11 (SOX11), and investigated senescence-related signaling pathways. With repeated passages, the number of senescent cells increased, while the cell proliferation capacity decreased; GATA6 mRNA expression was upregulated and that of SOX11 was downregulated. Repetitive cell passages decreased Wnt and bone morphogenetic protein (BMP) signaling pathway-related gene expression. Silencing of GATA6 and SOX11 regulated Wnt and BMP signaling pathway-related genes and affected cell senescence-related genes; moreover, SOX11 silencing regulated GATA6 expression. Hence, we identified them as pair of regulatory transcription factors for cell senescence in hMSCs via the Wnt and BMP signaling pathways.


Asunto(s)
Senescencia Celular/genética , Células de la Médula Ósea/citología , Proteínas Morfogenéticas Óseas/genética , Proteínas Morfogenéticas Óseas/metabolismo , Diferenciación Celular , Proliferación Celular , Células Cultivadas , Factor de Transcripción GATA6/antagonistas & inhibidores , Factor de Transcripción GATA6/genética , Factor de Transcripción GATA6/metabolismo , Histona Desacetilasa 2/genética , Histona Desacetilasa 2/metabolismo , Humanos , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Interferencia de ARN , ARN Interferente Pequeño/metabolismo , Factores de Transcripción SOXC/antagonistas & inhibidores , Factores de Transcripción SOXC/genética , Factores de Transcripción SOXC/metabolismo , Transducción de Señal/genética , Proteínas Wnt/genética , Proteínas Wnt/metabolismo
14.
Gen Comp Endocrinol ; 167(1): 135-42, 2010 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-20138183

RESUMEN

The responses of Mozambique and Nile tilapia acclimated to fresh water (FW) and brackish water (BW; 17 per thousand) were compared following acute salinity challenges. In both species, plasma osmolality increased to above 450 mOsm by 2h after transfer from FW to seawater (SW); these increases in osmolality were accompanied by unexpected increases in plasma prolactin (PRL). Likewise, PRL receptor gene expression in the gill also increased in both species. In Nile tilapia, hyperosmotic transfers (FW to BW and SW) resulted in increased plasma growth hormone (GH) and in branchial GH receptor gene expression, responses that were absent in Mozambique tilapia. Branchial gene expression of osmotic stress transcription factor 1 (OSTF1) increased in both species following transfer from FW to SW, whereas transfer from BW to SW induced OSTF1 expression only in the Nile tilapia. Branchial expression of Na(+)/Cl(-) cotransporter was higher in FW in both species than in BW. Branchial gene expression of Na(+)/K(+)/2Cl(-) cotransporter (NKCC) increased after transfer from BW to SW in Mozambique tilapia, whereas expression was reduced in the Nile tilapia following the same transfer. The difference in the SW adaptability of these species may be related to a limited capacity of Nile tilapia to up-regulate NKCC gene expression, which is likely to be an essential component in the recruitment of SW-type chloride cells. The differential responses of GH and OSTF1 may also be associated with the disparate SW adaptability of these two tilapiine species.


Asunto(s)
Cíclidos/sangre , Proteínas de Peces/genética , Regulación de la Expresión Génica , Hormona del Crecimiento/sangre , Prolactina/sangre , Salinidad , Tilapia/sangre , Animales , Péptidos y Proteínas de Señalización Intracelular , Péptidos/genética , Reacción en Cadena de la Polimerasa , Receptores de Prolactina/genética , Receptores de Somatotropina/genética
15.
Int J Sports Med ; 31(9): 656-70, 2010 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-20589593

RESUMEN

Moderate-intensity exercise at the lactate threshold (LT) is considered to be a safe and effective training regimen for improving metabolic syndrome. The aim of the current study was to investigate the effects of moderate exercise performed at the LT on skeletal muscle gene expression. 6 healthy men participated in cycle ergometer training at LT, 60 min/d, 5 d/wk for 12 wks. Muscle samples were collected after 5 d of training, and then 2 d after training at wks 6 and 12. Quantitative real-time PCR analysis revealed that the expression of peroxisome proliferator activated receptor co-activated 1alpha was significantly increased at 1 h after the training session on day 5. Moreover, using serial analysis gene expression, we found that moderate training for 6 and 12 wks simultaneously induced the expression of a number of metabolic genes involved in the TCA cycle, beta-oxidation, and electron transport. Furthermore, several genes encoding antioxidant enzymes and contractile apparatus were induced. The expression levels of 233 novel transcripts were also altered in response to moderate exercise. Thus, moderate training at the LT is a sufficient stimulus to induce the expression of numerous genes implicated in the development of metabolic syndrome, transcripts involved in the contractile apparatus, and novel transcripts.


Asunto(s)
Ciclismo/fisiología , Regulación de la Expresión Génica/fisiología , Músculo Esquelético/fisiología , Adulto , Umbral Anaerobio/fisiología , Ergometría , Ejercicio Físico/fisiología , Humanos , Ácido Láctico/sangre , Masculino , Reacción en Cadena de la Polimerasa , Adulto Joven
16.
Reproduction ; 138(2): 391-405, 2009 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-19494046

RESUMEN

The reproductive physiology of the Pacific white-sided dolphin, Lagenorhynchus obliquidens, was characterized to facilitate the development of artificial insemination (AI) using cryopreserved spermatozoa. Specific objectives were to: 1) describe reproductive seasonality of the Pacific white sided dolphins; 2) describe urinary LH and ovarian steroid metabolites during the estrous cycle; 3) correlate LH and ovarian steroidal metabolite patterns to ultrasound-monitored follicular growth and ovulation; and 4) assess the efficacy of synchronizing estrus, sperm collection/cryopreservation, and intrauterine insemination. Ovulations (64%, n=37) and conceptions (83%, n=18) occurred from August to October. Peak mean serum testosterone (24 ng/ml), cross-sectional testicular area (41.6 cm(2)), and sperm concentration (144.3 x 10(7) sperm/ml) occurred in July, August, and September respectively. Spermatozoa were only found in ejaculates from July to October. Estrous cycles (n=22) were 31 d long and were comprised of a 10 d follicular and 21 d luteal phase. Ovulation occurred 31.2 h after the onset of the LH surge and 19.3 h after the LH peak. Follicular diameter and circumference within 12 h of ovulation were 1.52 and 4.66 cm respectively. Estrus synchronization attempts with altrenogest resulted in 17 (22%) ovulatory cycles with ovulation occurring 21 d post-altrenogest. Ten AI attempts using cryopreserved semen resulted in five pregnancies (50%). The mean gestation length was 356 days (range 348-367). These data provide new information on the Pacific white-sided dolphin's reproductive physiology and collectively enabled the first application of AI in this species.


Asunto(s)
Delfines/fisiología , Ciclo Estral/fisiología , Reproducción/fisiología , Técnicas Reproductivas Asistidas/veterinaria , Estaciones del Año , Animales , Criopreservación/métodos , Criopreservación/veterinaria , Sincronización del Estro/métodos , Femenino , Inseminación Artificial/métodos , Inseminación Artificial/veterinaria , Hormona Luteinizante/orina , Masculino , Folículo Ovárico/diagnóstico por imagen , Ovulación , Embarazo , Resultado del Embarazo , Progesterona/agonistas , Preservación de Semen/métodos , Preservación de Semen/veterinaria , Recuento de Espermatozoides/veterinaria , Testículo/diagnóstico por imagen , Testosterona/sangre , Acetato de Trembolona/análogos & derivados , Acetato de Trembolona/uso terapéutico , Ultrasonografía
17.
Science ; 212(4494): 547-9, 1981 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-7209548

RESUMEN

Toxins in shellfish, which are responsible for paralytic poisonings, undergo reductive transformation when incubated with the homogenate of various portions of the scallop, Placopecten magellanicus. The transformation includes the reductive elimination of O-sulfate groups, a change that is most evident in the locomotor tissue homogenates. The commercially important adductor muscles can also inactivate the toxins.


Asunto(s)
Moluscos/metabolismo , Saxitoxina/metabolismo , Mariscos , Animales , Biotransformación , Inactivación Metabólica , Músculos/metabolismo , Oxidación-Reducción
18.
Kyobu Geka ; 62(2): 161-3, 2009 Feb.
Artículo en Japonés | MEDLINE | ID: mdl-19202940

RESUMEN

The patient was 55-years-old woman. She was referred to our hospital for an abnormal mass on a chest X-ray. She was pointed out it 12 years ago, but left it unattended. Therefore, we could observe the development of Castleman's disease for 12 years. An enhanced computed tomography showed a irregularly enhanced mass in the right cardiophrenic angle. We diagnosed as pleural tumor and performed the video assisted operation. The mass was growing from the anterior mediastinum. It was successfully resected thoracoscopically. The mass was elastic and hard, and 6.0 x 4.5 x 3.4 cm in size. The histopathological diagnosis was Castleman's disease, hyaline vascular type. We think that the tumor of Castleman's disease in the cadiophrenic angle may be resected by the thoracoscopic procedure with minimal thoracotomy.


Asunto(s)
Enfermedad de Castleman/cirugía , Mediastino , Cirugía Torácica Asistida por Video , Enfermedad de Castleman/diagnóstico , Enfermedad de Castleman/patología , Femenino , Humanos , Persona de Mediana Edad , Resultado del Tratamiento
19.
J Steroid Biochem Mol Biol ; 111(3-5): 225-31, 2008 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-18602997

RESUMEN

Breast cancer is the most common cancer among women. Androgens, the male sexual hormones produced by ovary, act as protector of mammary gland. To elucidate the possible effects of dihydrotestosterone (DHT) on the transcriptome of mammary gland, serial analysis of gene expression was carried out on three groups of gonadectomized mice. After gonadectomy (GDX), DHT was injected 3 or 24h before sacrifice, whereas the control (GDX) group received vehicle solution. Approximately 42,000 tags were sequenced in each group. Genes involved in the cytoskeletal and extracellular matrix, such as troponin I skeletal fast 2 and keratin complex 1 acidic gene 14, were upregulated. In the immunity, complement component 1 q subcomponent gamma polypeptide and expressed sequence tag similar to lectin galactose binding soluble 3 were downregulated by DHT, whereas serine (or cystein) proteinase inhibitor clade A member 1a was upregulated. In the energy metabolism, the gene expression level of cytochrome c oxidase subunit I was upregulated by DHT, while NADH dehydrogenase subunit 2 was downregulated. In addition, transcripts involved in transport metabolism, such as apolipoprotein A-1, were upregulated by DHT, whereas retinol binding protein 4 plasma was downregulated. Several previously unknown sequence tags were identified, which may allow to characterize new molecules of interest. These results suggest the suppression of immune response in normal mammary gland after DHT injection. This study can assist in refining research on the role of androgens in mammary gland homeostasis and breast cancer.


Asunto(s)
Dihidrotestosterona/farmacología , Expresión Génica/efectos de los fármacos , Glándulas Mamarias Animales , Animales , Neoplasias de la Mama/genética , Femenino , Perfilación de la Expresión Génica/métodos , Humanos , Masculino , Glándulas Mamarias Animales/efectos de los fármacos , Glándulas Mamarias Animales/fisiología , Ratones , Ratones Endogámicos C57BL , Orquiectomía , Ovariectomía , Transcripción Genética
20.
J Periodontal Res ; 43(2): 174-8, 2008 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-18302619

RESUMEN

BACKGROUND AND OBJECTIVE: The effects of laser irradiation on Porphyromonas gingivalis have been reported, but the results are still controversial regarding the efficiency because of the differences of the light sources and irradiation conditions. The aim of this study was to determine the wavelength and irradiation conditions under which the most effective inhibitory effect on P. gingivalis growth was seen without any photosensitizers. MATERIAL AND METHODS: Using an Okazaki large spectrograph, monochromatic light spectra ranging from 400 to 700 nm were evaluated to determine which spectra effectively inhibited bacterial growth. Moreover, using a monochromatic 405-nm irradiating device, the effects of various irradiating conditions on P. gingivalis growth were examined. RESULTS: Growth of bacteria irradiated at 400 nm and 410 nm was significantly suppressed compared with a nonirradiated control, whereas wavelengths of 430 nm and longer produced no significant inhibition. A constant energy density of 15 J/cm2 was found to be enough to show an inhibitory effect. Significant inhibition of bacterial growth was found after only 1 min at 50 mW/cm2 irradiation. CONCLUSION: These results indicate that P. gingivalis growth is specifically suppressed by 405-nm light irradiation, suggesting that visible blue light irradiation is a promising means for eradicating periodontopathogenic bacteria from periodontal lesions.


Asunto(s)
Luz , Porphyromonas gingivalis/efectos de la radiación , Recuento de Colonia Microbiana , Viabilidad Microbiana/efectos de la radiación , Porphyromonas gingivalis/crecimiento & desarrollo , Espectrofotometría
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