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1.
Zhongguo Zhong Yao Za Zhi ; 46(19): 4950-4958, 2021 Oct.
Artículo en Zh | MEDLINE | ID: mdl-34738389

RESUMEN

In this study, the gene encoding the key enzyme 3-ketoacyl-CoA thiolase(KAT) in the fatty acid ß-oxidation pathway of Atractylodes lancea was cloned. Meanwhile, bioinformatics analysis, prokaryotic expression and gene expression analysis were carried out, which laid a foundation for the study of fatty acid ß-oxidation mechanism of A. lancea. The full-length sequence of the gene was cloned by RT-PCR with the specific primers designed according to the sequence information of KAT gene in the transcriptomic data of A. lancea and designated as AIKAT(GenBank accession number MW665111). The results showed that the open reading frame(ORF) of AIKAT was 1 323 bp, encoding 440 amino acid. The deduced protein had a theoretical molecular weight of 46 344.36 and an isoelectric point of 8.92. AIKAT was predicted to be a stable alkaline protein without transmembrane segment. The secondary structure of AIKAT was predicted to be mainly composed of α-helix. The tertiary structure of AIKAT protein was predicted by homology modeling method. Homologous alignment revealed that AIKAT shared high sequence identity with the KAT proteins(AaKAT2, CcKAT2, RgKAT and AtKAT, respectively) of Artemisia annua, Cynara cardunculus var. scolymus, Rehmannia glutinosa and Arabidopsis thaliana. The phylogenetic analysis showed that AIKAT clustered with CcKAT2, confirming the homology of 3-ketoacyl-CoA thiolase genes in Compositae. The prokaryotic expression vector pET-32 a-AIKAT was constructed and transformed into Escherichia coli BL21(DE3) for protein expression. The target protein was successfully expressed as a soluble protein of about 64 kDa. A real-time quantitative PCR analysis was performed to profile the AIKAT expression in different tissues of A. lancea. The results demonstrated that the expression level of AIKAT was the highest in rhizome, followed by that in leaves and stems. In this study, the full-length cDNA of AIKAT was cloned and expressed in E. coli BL21(DE3), and qRT-PCR showed the differential expression of this gene in different tissues, which laid a foundation for further research on the molecular mechanism of fatty acid ß-oxidation in A. lancea.


Asunto(s)
Atractylodes , Secuencia de Aminoácidos , Atractylodes/genética , Clonación Molecular , Coenzima A , Escherichia coli/genética , Filogenia
2.
Mediators Inflamm ; 2018: 3093516, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29853787

RESUMEN

Depression is a common mental disorder in modern society. A traditional Chinese medicine Huanglian-Wendan decoction with potential anti-inflammation is used as a clinical antidepressant. Our previous study showed central and peripheral inflammatory responses in a rat model of depression developed by chronic unpredictable mild stress (CUMS). Here, we investigated the anti-inflammatory activity and mechanism of Huanglian-Wendan decoction in CUMS rats. LC-MS/MS and HPLC were performed to determine the major compounds in water extract of this decoction. This study showed that Huanglian-Wendan decoction significantly increased sucrose consumption and reduced serum levels of interleukin-1 beta (IL-1ß), IL-6, and alanine aminotransferase (ALT) in CUMS rats. Moreover, this decoction inhibited nuclear entry of nuclear factor-kappa B (NF-κB) with the reduction of phosphorylated protein of NF-κB (p-NF-κB) and inhibitor of NF-κB alpha (p-IκBα) and downregulated protein of nod-like receptor family pyrin domain-containing 3 (NLRP3), apoptosis-associated speck-like protein containing CARD (ASC), cysteinyl aspartate-specific proteinase-1 (Caspase-1), and IL-1ß in liver and brain regions of CUMS rats. These findings demonstrated that Huanglian-Wendan decoction had antidepressant activity with hepatoprotection in CUMS rats coinciding with its anti-inflammation in both periphery and central. The inhibitory modulation of NF-κB and NLRP3 inflammasome activation by Huanglian-Wendan decoction may mediate its antidepressant action.


Asunto(s)
Encéfalo/metabolismo , Medicamentos Herbarios Chinos/farmacología , Inflamasomas/efectos de los fármacos , Inflamasomas/metabolismo , Hígado/metabolismo , FN-kappa B/metabolismo , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Animales , Western Blotting , Encéfalo/efectos de los fármacos , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Hígado/efectos de los fármacos , Masculino , Ratas
3.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(5): 1592-7, 2016 05.
Artículo en Inglés | MEDLINE | ID: mdl-30001069

RESUMEN

Rock-mineral spectrum is a mixture of varied mineral spectra, through which we can obtain information about its components quickly and conveniently without any damage to the sample. Empirical mode decomposition (EMD) cannot directly decompose source signals from information of the mixture, and independent component analysis (ICA) requires the number of mixed signals to be no less than the number of source signals. Combining these two methods, mixed signals can be decomposed using EMD method to obtain intrinsic mode function (IMF), while certain IMFs together with mixed signals can be used as input data matrix of ICA to obtain the source signals. This method overcomes the shortcomings of IMF and ICA. Studies have shown that, the higher content of source signals contained in the mixed signal, the better estimation can be obtained through EMD and ICA. The number of IMFs that participate in ICA decomposition determines the number of approximation of source signals. The accuracy of source signal estimation increases with the correlation coefficient between IMF and mixed signals. By applying this method to quantitative analysis of rock-mineral spectrum, information of the component minerals in rock-mineral can be obtained, which improves the efficiency of component analysis in detecting rock-minerals outside.

4.
Redox Biol ; 18: 124-137, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-30014902

RESUMEN

Oxidative stress is a critical factor in nonalcoholic fatty liver disease pathogenesis. MicroRNA-200a (miR-200a) is reported to target Kelch-like ECH-associated protein 1 (Keap1), which regulates nuclear factor erythroid 2-related factor 2 (Nrf2) anti-oxidant pathway. Polydatin (3,4',5-trihydroxy-stilbene-3-ß-D-glucoside), a polyphenol found in the rhizome of Polygonum cuspidatum, have anti-oxidative, anti-inflammatory and anti-hyperlipidemic effects. However, whether miR-200a controls Keap1/Nrf2 pathway in fructose-induced liver inflammation and lipid deposition and the blockade of polydatin are still not clear. Here, we detected miR-200a down-regulation, Keap1 up-regulation, Nrf2 antioxidant pathway inactivation, ROS-driven thioredoxin-interacting protein (TXNIP) over-expression, NOD-like receptor (NLR) family, pyrin domain containing 3 (NLRP3) inflammasome activation and dysregulation of peroxisome proliferator activated receptor-α (PPAR-α), carnitine palmitoyl transferase-1 (CPT-1), sterol regulatory element binging protein 1 (SREBP-1) and stearoyl-CoA desaturase-1 (SCD-1) in rat livers, BRL-3A and HepG2 cells under high fructose induction. Furthermore, the data from the treatment or transfection of miR-200a minic, Keap1 and TXNIP siRNA, Nrf2 activator and ROS inhibitor demonstrated that fructose-induced miR-200a low-expression increased Keap1 to block Nrf2 antioxidant pathway, and then enhanced ROS-driven TXNIP to activate NLRP3 inflammasome and disturb lipid metabolism-related proteins, causing inflammation and lipid deposition in BRL-3A cells. We also found that polydatin up-regulated miR-200a to inhibit Keap1 and activate Nrf2 antioxidant pathway, resulting in attenuation of these disturbances in these animal and cell models. These findings provide a novel pathological mechanism of fructose-induced redox status imbalance and suggest that the enhancement of miR-200a to control Keap1/Nrf2 pathway by polydatin is a therapeutic strategy for fructose-associated liver inflammation and lipid deposition.


Asunto(s)
Antiinflamatorios/uso terapéutico , Fructosa/efectos adversos , Glucósidos/uso terapéutico , Inflamación/inducido químicamente , Inflamación/prevención & control , Hígado/efectos de los fármacos , MicroARNs/inmunología , Estilbenos/uso terapéutico , Animales , Antioxidantes/uso terapéutico , Línea Celular , Medicamentos Herbarios Chinos/uso terapéutico , Inflamación/inmunología , Inflamación/patología , Proteína 1 Asociada A ECH Tipo Kelch/inmunología , Lípidos/análisis , Lípidos/inmunología , Hígado/inmunología , Hígado/patología , Masculino , Factor 2 Relacionado con NF-E2/inmunología , Estrés Oxidativo/efectos de los fármacos , Ratas Sprague-Dawley , Transducción de Señal/efectos de los fármacos
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