RESUMEN
Genomic DNA isolation is a crucial technique for researchers studying plant molecular biology. A current widely-used protocol for DNA extraction needs a pestle and mortal for each sample and consumes a large amount of liquid nitrogen in grinding the samples. Most high-throughput methods depend on expensive machines for tissue homogenization. Here we developed a CTAB-based DNA extraction method using 2.0â¯mL microcentrifuge tubes for sample processing. This protocol has the advantages that it is suitable for a variety of plants, easily-performed without special equipment, and high-throughput; it effectively avoids sample cross-contamination, and is inexpensive, rapid and safe.
Asunto(s)
ADN de Plantas/aislamiento & purificación , Genoma de Planta , Ensayos Analíticos de Alto Rendimiento/métodos , Plantas/genética , Arabidopsis/genética , ADN de Plantas/análisis , Electroforesis en Gel de Agar , Extracción Líquido-LíquidoRESUMEN
In the title compound, C(14)H(15)NO(2), the dihedral angle between the two benzene rings is 71.10â (5)°. In the crystal, mol-ecules are linked by inter-molecular N-Hâ¯O, and O-Hâ¯N hydrogen bonds into a chain running parallel to the b axis.