Bioaccessibility of lead and cadmium in soils around typical lead-acid power plants and their effect on gut microorganisms.

Potentially toxic elements (Pb and Cd) contamination of soil can adversely affect human health. Moreover, these metal ions interact with the gut microbiota after entering the human digestive system. Based on the physiologically based extraction test and the simulator of human intestinal microbial ecosystem, the bioaccessibility of Pb and Cd in soils contaminated with lead-acid power plants was assessed. The gastric stage exhibited the greatest average bioaccessibility of lead and cadmium (63.39% and 57.22%), followed by the small intestinal stage (6.86% and 36.29%); due to gut microorganisms, the bioaccessibility of lead and cadmium was further reduced in the colon stage (1.86% and 4.22%). Furthermore, to investigate soil contamination's effects on gut microbes, 16S rRNA high-throughput sequencing was used to identify the gut microbial species after the colon period. Due to Pb and Cd exposure, the relative abundance of Firmicutes and unidentified_Bacteria decreased, while the relative abundance of Proteobacteria, Synergistota, and Bacteroidota increased. The relationship between environmental factors and the number of microbial species in the gut was also examined using Spearman correlation analysis. Pb and Cd exposure has been found to affect the composition and structure of the gut microbiota.

Contrasting effects of a novel biochar-microalgae complex on arsenic and mercury removal.

Biochar and algae were commonly used as environmental-friendly adsorbents to treat wastewater contaminated with heavy metals. In the study, we used a biochar-microalgae complex of Coconut shell activated carbon (Csac) and Chlorella to evaluate and compare the adsorption ability of arsenic and mercury. The adsorption kinetic study showed that the adsorption efficiency of the biochar-microalgae complex for mercury was better remarkably than arsenic (about 74.84% higher in initial 1 min and 71.62% higher at adsorption equilibrium), which could be interpreted as the complex had excellent adsorption capacity for mercury. The new biochar-microalgae complex adsorbed up to 46.8 µg·g-1 of mercury at 100 µg·L-1 concentration. FTIR and XPS indicated that the surface of biochar-microalgae complex adsorbent had abundant oxygen-containing functional groups that could provide active sites during the adsorption process, i.e., -COOH, -OH and C-O-C et al. Compared with arsenic, the adsorption peaks of mercury moved or changed significantly, suggesting that the complex strongly adsorbed mercury and the main adsorption mechanisms were the ion exchange and complexation between functional groups and mercury ion. What must be emphasized was arsenic mainly existed as negative ions (AsO2-, AsO23-) in water, which was the reason for the weak adsorption capacity of the biochar-microalgae complex for arsenic. In short, the adsorption efficiency and performance of the biochar-microalgae complex was significantly higher than that of arsenic (p < 0.01), and the adsorption of mercury by biochar-microalgae was chemisorption based on the single molecular layer theory.

Study on arsenic speciation, bioaccessibility, and gut microbiota in realgar-containing medicines by DGT technique and artificial gastrointestinal extraction (PBET) combine with simulated human intestinal microbial ecosystem (SHIME).

It is well-known that several Chinese patent medicines use realgar as a specific component. People are more aware of the health dangers associated with realgar since it includes arsenic. Previous research overstated the arsenic toxicity of realgar-containing Chinese prescription medications because little thought was given to the influence of arsenic bioaccessibility by gut microbiota. In light of this, this study examined the total content, bioaccessibility and speciation of targeted medications while also examining intestinal epithelial transit utilizing the diffusive gradients in thin-films (DGT). All samples contained arsenic, and the bioaccessibilities of the colon, intestine and gastric regions ranged from 0.19% to 1.73%, 0.25-1.88% and 0.21-1.70% respectively. The range of DGT-bioaccessibility is 0.01-0.0018%. Three steps of analysis were conducted on inorganic As(III) and As(V). In health risk assessment, the ADDs and HQs of DGT-bioaccessibility were below the threshold levels when compared to computing average daily intake dose (ADD) and hazard quotient (HQ) by bioaccessibility of gastric, intestinal and colon. Additionally, Proteobacteria and Firmicutes were discovered to be the two predominant kinds of gut microbes in this study. Under arsenic exposure, the abundance of Christensenellaceae, Desulfovibrionaceae and Akkermansiaceae increased, but the quantity of Rikenellaceae decreased. These findings revealed that alterations in gut microbiota had an impact on host metabolism.

Improved effects of combined application of nitrogen-fixing bacteria Azotobacter beijerinckii and microalgae Chlorella pyrenoidosa on wheat growth and saline-alkali soil quality.

Soil salinization seriously affects crop yield and soil productivity. The application of bacteria and microalgae has been considered as a promising strategy to alleviate soil salinization. However, the effect of bacteria-microalgae symbiosis on saline-alkali land is still unclear. This study evaluated the effects of Azotobacter beijerinckii, Chlorella pyrenoidosa, and their combined application on the wheat growth and saline-alkali soil improvement. The results showed that, among all the treatments, A. beijerinckii + live C. pyrenoidosa combined inoculation group (BA) had the best effect on increasing wheat plant biomass, improving salt tolerance, and improving soil fertility. The dry weight of wheat plant in the BA group increased by 66.7%, 17.4%, and 35.0%, respectively, compared with the control group (CK), A. beijerinckii inoculation group (B), and live C. pyrenoidosa inoculation group (A). The total nitrogen content of wheat plant in the BA group increased by 69.5%, 76.7%, and 71.1%, compared with the CK, B, and A group. The proline content of wheat plant in the BA group was 100% higher than that in the CK group. The N/P ratio and K/Na ratio of wheat plant increased by 157% and 12.9% in the BA group compared with the CK group, respectively, which was more conducive to alleviating nitrogen limitation and salt stress. The A. beijerinckii + live C. pyrenoidosa inoculation treatment better reduced soil pH and improved the availability of phosphorus in soil. This study illustrated the comprehensive application prospects of bacteria-microalgae interactions on wheat growth promotion and soil improvement in saline-alkali land, and provided a new effective strategy for improving saline-alkali soil quality and increasing crop productivity.

Study on the mechanism of biochar loaded typical microalgae Chlorella removal of cadmium.

Coconut shell activated carbon (Csac) is one of the most widely used materials to remove cadmium (Cd) from contaminated water. A large diversity of microorganisms exists in various aquatic systems and may aid Cd removal by Csac. In this study, we explored the reactions of Csac with microalgae (Chlorella) in Cd-containing media. The results of scanning electron microscope (SEM) imaging, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), superconducting pulse-Fourier transform nuclear magnetic resonance (pulse-FT NMR) and X-ray photoelectron spectroscopy (XPS) indicated that Chlorella could adhere in the micropores of Csac formed Csac@Chlorella composite adsorbent loading Chlorella. Furthermore, the composite adsorbent surface had abundant functional groups such -COOH, -OH and C-O-C, which served as active sites during the adsorption process. Compared with Csac, Csac@Chlorella had an enhanced Cd adsorption capacity evidently. The results showed that pH 8, 0.2 g Csac, OD680 of 0.1 for Chlorella were optimal conditions for maximum Cd adsorption capacity within one hour contact time. Furthermore, the Cd adsorption process was well described by the pseudo-second-order and Langmuir adsorption isotherm models. The models revealed that the adsorption process was mainly based on chemical adsorption of a single molecular layer, accompanied by electrostatic attraction, complexation and intracellular adsorption, amongst other parameters. Collectively, the findings illustrate that the microalgae (Chlorella)-Csac-Cd interaction is complex and will thus have immense interest to a broad range of biological, environmental, and geoscience communities.

[Surgical treatment of open fractures of the talus].

OBJECTIVE: To evaluate the long-term outcomes of surgical treatment of open fracture of talus. METHODS: Sixteen patients with displaced open fractures of the talus, including 3 cases of type 1, 8 of type 2, 2 of type 3A, 2 of type 3B, and 1 of type 3C according to the Gustilo-Anderson classification system, 15 males and 1 female, aged 37.5 (21 - 48), were treated with open reduction and stable internal fixation within 6 - 8 hours after injury, and followed up for 3.2 years (3 - 6 years). There were 7 cases of pure displaced talar neck or body fracture and 9 cases of fractures associated with an ipsilateral pilon, ankle, and calcaneus totally that included 2 cases of type 2, 3 of type 3, and 1 of type 4 according to the Hawkins classification system modified by Canale. RESULTS: The union rate was 81% (13/16) with the average union time of 4.5 months. Three cases failed to heal and developed osteomyelitis, of which 2 were treated with late talectomy because of septic nonunion. One case underwent amputation below-the-knee because of instability and pain. Osteonecrosis developed after seven of the nineteen closed fractures, whereas posttraumatic arthritis developed after all nineteen. Osteonecrosis developed in 11 (68%), and posttraumatic arthritis developed in 13 of the 16 cases (85%). The average American Orthopaedic Foot and Ankle Society score of these patients was 43.7 points. CONCLUSION: Open fractures of the talus has a low union rate, a higher osteonecrosis rate, and substantially higher reoperation and infection rates. Posttraumatic arthritis is a more common complication than osteonecrosis following operative treatment. Open reduction and internal fixation are recommended for the treatment of open fractures of talus.