Ratio of Overhydration and Extracellular Water Versus Ratio of Extracellular Water and Body Cell Mass in the Assessment of Fluid Status in Patients With Acute Kidney Injury Requiring Kidney Replacement Therapy: A Cohort Study.

OBJECTIVES: The aim of this study is to analyze the association between the ratio of overhydration and extracellular water (OH/ECW) and the ratio of extracellular water and body cell mass (ECW/BCM) measured by bioelectrical impedance and outcomes of patients with acute kidney injury (AKI) requiring kidney replacement therapy (KRT). METHODS: Patients with severe AKI treated with KRT in our hospital between September 2016 and August 2018 were enrolled. These patients were assessed using a body composition monitor before KRT, and on the 3rd day and the 7th day after initiation of KRT. The predictors mainly included OH/ECW and ECW/BCM. The association between all-cause mortality and predictors were analyzed using Cox regression. RESULTS: A total of 152 patients were included in this study with a median follow-up of 39 (interquartile range 8-742) days. The 28-day mortality, 90-day mortality, and 1-year mortality were 46.7%, 54.6%, and 60.5%, respectively. A high ratio of OH/ECW (adjusted hazard ratio per standard deviation, 1.45; 95% confidence interval = 1.15-1.82, P = .002) and a high ratio of ECW/BCM (adjusted hazard ratio per standard deviation, 1.33, 95% confidence interval = 1.07-1.64, P = .009) before KRT were associated with all-cause mortality during follow-up. Higher ECW/BCM rather than OH/ECW at 7th day was associated with poorer outcomes. Furthermore, a reduction of OH/ECW with an increase of ECW/BCM had higher 1-year mortality as compared to others (85.7% vs. 51.2%, P = .004) in patients who survived 7 days after KRT initiation. CONCLUSIONS: ECW/BCM performed better than OH/ECW in assessment of fluid status in AKI patients requiring KRT. This study suggested that a simple reduction of OH/ECW without decreasing ECW/BCM may not improve outcomes.

Body Composition Analysis as a Predictor of Prognosis for Patients With Acute Kidney Injury Requiring Kidney Replacement Therapy.

OBJECTIVES: The aim of this study is to investigate the association between body composition, measured by bioelectrical impedance analysis, and outcomes in patients with acute kidney injury (AKI) receiving kidney replacement therapy (KRT). METHODS: Patients with severe AKI treated with KRT in our hospital between September 2016 and August 2018 were enrolled. These patients were assessed by body composition analysis before KRT, and on the 3rd day and the 7th day after initiation of KRT. The predictors included lean tissue index (LTI), fat tissue index, and body cell mass index (BCMI). The association between all-cause mortality and predictors was analyzed using Cox regression. RESULTS: A total of 152 patients were included in this study, with a 28-day mortality of 46.7% and 1-year mortality of 60.5%. LTI (adjusted hazard ratio per standard deviation: 0.37; 95% confidence interval = 0.21-0.66, P < .001) and BCMI (adjusted hazard ratio per standard deviation: 0.37; 95% confidence interval = 0.21-0.67, P < .001) on day 7 after initiation of KRT, rather than before KRT, were associated with mortality during follow-up. LTI and BCMI before KRT were associated with 28-day mortality rather than 1-year mortality. CONCLUSIONS: LTI and BCMI before KRT were associated with short-term prognosis, and those on day 7 after KRT initiation were associated with intermediate mortality in patients with AKI requiring KRT.

Transparent Microcrystalline Cellulose/Polyvinyl Alcohol Paper as a New Platform for Three-Dimensional Cell Culture.

Multilayered and stacked cellulose paper has emerged as a promising platform for construction of three-dimensional (3D) cell culture because of its low cost, good biocompatibility, and high porosity. However, its poor light transmission makes it challenging to directly and clearly monitor cell behaviors (e.g., growth and proliferation) on the paper-based platform using an optical microscope. In this work, we developed a transparent microcrystalline cellulose/polyvinyl alcohol (MCC/PVA) paper with irregular pores through dissolution and regeneration of microcrystalline nanocellulose, addition of a porogen reagent (NaCl), and subsequently dipping in PVA solutions. The transparent MCC paper displays high porosity (up to 90%), adjustable pore size (between 23 and 46 µm), large thickness (from 315 to 436 µm), and high light transmission under water (>95%). Through further modification of the transparent MCC paper with PVA, the obtained transparent MCC/PVA paper shows enhanced mechanical properties (dry and wet strengths), good hydrophilicity (with a contact angle of 70.8°), and improved biocompatibility (cell viability up to 90%). By stacking and destacking multiple layers of the transparent MCC/PVA paper, it has been used for both two-dimensional and three-dimensional cell culture platforms. The transparent MCC/PVA paper under water enables both direct observation of cell morphology by an optical microscope via naked eyes and fluorescence microscope after staining. We envision that the developed transparent MCC/PVA paper holds great potential for future applications in various bioanalytical and biomedical fields, such as drug screening, tissue engineering, and organ-on-chips.

Recent advances in cellulose-based membranes for their sensing applications.

ABSTRACT: In recent years, sensing applications have played a very important role in various fields. As a novel natural material, cellulose-based membranes with many merits can be served as all kinds of sensors. This review summarizes the recent progress of cellulose membranes as sensors, mainly focusing on their preparation processes and sensing properties. In addition, the opportunities and challenges of cellulose membrane-based sensors are also prospected. This review provides some references for the design of cellulose membrane materials for sensing applications in the future.

Preparation of Magnetic CuFe2O4@Ag@ZIF-8 Nanocomposites with Highly Catalytic Activity Based on Cellulose Nanocrystals.

A facile approach was successfully developed for synthesis of cellulose nanocrystals (CNC)-supported magnetic CuFe2O4@Ag@ZIF-8 nanospheres which consist of a paramagnetic CuFe2O4@Ag core and porous ZIF-8 shell. The CuFe2O4 nanoparticles (NPs) were first prepared in the presence of CNC and dispersant. Ag NPs were then deposited on the CuFe2O4/CNC composites via an in situ reduction directed by dopamine polymerization (PDA). The CuFe2O4/CNC@Ag@ZIF-8 nanocomposite was characterized by TEM, FTIR, XRD, N2 adsorption-desorption isotherms, VSM, and XPS. Catalytic studies showed that the CuFe2O4/CNC@Ag@ZIF-8 catalyst had much higher catalytic activity than CuFe2O4@Ag catalyst with the rate constant of 0.64 min-1. Because of the integration of ZIF-8 with CuFe2O4/CNC@Ag that combines the advantaged of each component, the nanocomposites were demonstrated to have an enhanced catalytic activity in heterogeneous catalysis. Therefore, these results demonstrate a new method for the fabrication of CNC-supported magnetic core-shell catalysts, which display great potential for application in biocatalysis and environmental chemistry.

Capillary blood for point-of-care testing.

Clinically, blood sample analysis has been widely used for health monitoring. In hospitals, arterial and venous blood are utilized to detect various disease biomarkers. However, collection methods are invasive, painful, may result in injury and contamination, and skilled workers are required, making these methods unsuitable for use in a resource-limited setting. In contrast, capillary blood is easily collected by a minimally invasive procedure and has excellent potential for use in point-of-care (POC) health monitoring. In this review, we first discuss the differences among arterial blood, venous blood, and capillary blood in terms of the puncture sites, components, sample volume, collection methods, and application areas. Additionally, we review the most recent advances in capillary blood-based commercial products and microfluidic instruments for various applications. We also compare the accuracy of microfluidic-based testing with that of laboratory-based testing for capillary blood-based disease diagnosis at the POC. Finally, we discuss the challenges and future perspectives for developing capillary blood-based POC instruments.

Engineered nanomedicine for myeloma and bone microenvironment targeting.

Bone is a favorable microenvironment for tumor growth and a frequent destination for metastatic cancer cells. Targeting cancers within the bone marrow remains a crucial oncologic challenge due to issues of drug availability and microenvironment-induced resistance. Herein, we engineered bone-homing polymeric nanoparticles (NPs) for spatiotemporally controlled delivery of therapeutics to bone, which diminish off-target effects and increase local drug concentrations. The NPs consist of poly(D,L-lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG), and bisphosphonate (or alendronate, a targeting ligand). The engineered NPs were formulated by blending varying ratios of the synthesized polymers: PLGA-b-PEG and alendronate-conjugated polymer PLGA-b-PEG-Ald, which ensured long circulation and targeting capabilities, respectively. The bone-binding ability of Ald-PEG-PLGA NPs was investigated by hydroxyapatite binding assays and ex vivo imaging of adherence to bone fragments. In vivo biodistribution of fluorescently labeled NPs showed higher retention, accumulation, and bone homing of targeted Ald-PEG-PLGA NPs, compared with nontargeted PEG-PLGA NPs. A library of bortezomib-loaded NPs (bone-targeted Ald-Bort-NPs and nontargeted Bort-NPs) were developed and screened for optimal physiochemical properties, drug loading, and release profiles. Ald-Bort-NPs were tested for efficacy in mouse models of multiple myeloma (MM). Results demonstrated significantly enhanced survival and decreased tumor burden in mice pretreated with Ald-Bort-NPs versus Ald-Empty-NPs (no drug) or the free drug. We also observed that bortezomib, as a pretreatment regimen, modified the bone microenvironment and enhanced bone strength and volume. Our findings suggest that NP-based anticancer therapies with bone-targeting specificity comprise a clinically relevant method of drug delivery that can inhibit tumor progression in MM.

Nanocellulose/carbon nanotube/manganese dioxide composite electrodes with high mass loadings for flexible supercapacitors.

The increasing commercialization of flexible electronic products has sparked a rising interest in flexible wearable energy storage devices. Supercapacitors are positioned as one of the systems with the most potential due to their distinctive advantages: high power density, rapid charge and discharge rates, and long cycle life. However, electrode materials face challenges in providing excellent mechanical strength while ensuring sufficient energy density. This study presents a method for constructing a flexible composite electrode material with high capacitance and mechanical performance by electrochemically depositing high-quality manganese dioxide (MnO2) onto the surface of a nanocellulose (CNF) and carbon nanotube (CNT) conductive film. In this electrode material, the CNF/CNT composite film serves as a flexible conductive substrate, offering excellent mechanical properties (modulus of 3.3 GPa), conductivity (55 S/cm), and numerous active sites. Furthermore, at the interface between MnO2 and the CNF/CNT substrate, C-O-Mn bonds are formed, promoting a tight connection between the composite materials. The assembled symmetric flexible supercapacitor (FSC) demonstrates impressive performance, with an areal specific capacitance of 934 mF/cm2, an energy density of 43.10 Wh/kg, a power density of 166.67 W/kg and a long cycle life (85 % Capacitance retention after 10,000 cycles), suggesting that they hold promise for FSC applications.

Rapid self-healing carboxymethyl chitosan/hyaluronic acid hydrogels with injectable ability for drug delivery.

In this study, the quaternized carboxymethyl chitosan (QCMCS), oxidized hyaluronic acid (OHA), 3,3'-dithiobis-(propionohydrazide) (DTP) were used as raw materials for the synthesis of hydrogels with excellent properties as carriers for drug release. The hydrogels were prepared by a simple "one-pot" method without external stimuli on the basis of interactions between formed dynamic covalent bonds (imine bonds, acylhydrazone bonds, disulfide bonds) and hydrogen bonds. The hydrogels had rapid self-healing properties, with a self-healing rate of 96 % after 30 min, as well as good pH responsiveness and excellent cytocompatibility (up to 98 % cell survival). The compressive stress of the hydrogels reached 423 kPa. Moreover, a representative drug (acetylsalicylic acid) demonstrated sustained release in the hydrogels (>72 h). The drug release behaviour was shown to be consistent with the Fick diffusion mechanism by kinetic modelling. Collectively, the findings demonstrate that the QCMCS + OHA + DTP injectable self-healing hydrogels are a potential material for the construction of pH-controlled drug delivery platforms.

Ultra-strong and tough cellulose-based conductive hydrogels via orientation inspired by noodles pre-stretching.

Due to the unsatisfactory mechanical properties of natural polymer-based conductive hydrogels, their applications are limited. Shaanxi Biangbiang noodles can be toughened by applying external mechanical forces through stretching and beating movements; this process provides inspiration for the preparation of high-strength hydrogels. In this paper, we propose a strategy for the preparation of ultrastrong and ultratough conductive hydrogels by directional prestretching and solvent exchange. Neatly arranged fiber bundles containing many intermolecular hydrogen bonds and metal ion coordination bonds are successfully constructed inside the prepared hydrogels. The hydrogel has exceptional mechanical properties, with a fracture stress exceeding 50 MPa, fracture strain approaching 105 %, fracture toughness exceeding 30 MJ m-3, and high conductivity reaching 11.738 ± 0.06 mS m-1. Impressively, the hydrogel can maintain its high mechanical properties after being frozen at an ultralow temperature of -80 °C for 7 days. Compared with other tough hydrogels, natural tendons and synthetic rubbers, the hydrogel exhibits excellent mechanical properties. The cellulose-based conductive hydrogel prepared in this study can be applied to robotic soft tissues (such as the Achilles tendon) that require high strength and are operated in extreme environments.

Cinobufacini enhances the therapeutic response of 5-Fluorouracil against gastric cancer by targeting cancer stem cells via AKT/GSK-3ß/ß-catenin signaling axis.

BACKGROUND: Gastric cancer stem cells (GCSCs) play crucial role in the development, recurrence, and resistance of gastric cancer (GC). Cinobufacini, a traditional Chinese medicine, offers significant advantages in improving tumor therapy. However, pre-clinical investigation into the antitumor effect and mechanism of Cinobufacini on GC is still lacking. Additionally, it has not been reported whether Cinobufacini is related to cancer stem cells (CSCs). METHODS: The CCK-8, clone formation, EdU staining, transwell and wound healing experiments were performed to assess the cell toxicity of Cinobufacini and demonstrate the preventive effects of Cinobufacini on proliferation, invasion, and migration of GC cells. Elucidating the underlying mechanism of Cinobufacini in GC based on the transcriptome sequencing. Flow cytometry assays, sphere formation assays, subcutaneous xenograft model in nude mice, and immunofluorescent staining have been used to investigate whether the anti-GC effect of Cinobufacini is associated with GCSCs and enhancing therapeutic response to 5-Fluorouracil (5-FU). RESULTS: Cinobufacini exerts minimal impact on normal human gastric epithelium cell GES-1, while significantly suppressing the proliferation, invasion, and migration of GC cell lines. Additionally, Cinobufacini attenuates the stemness of GCSCs by disrupting the AKT/GSK-3ß/ß-catenin signaling cascade. Moreover, Cinobufacin enhances the anti-tumor effects of 5-FU against GCSCs by reducing in vitro sphere formation and inhibiting subcutaneous graft tumor growth in vivo. CONCLUSIONS: Cinobufacini enhances the therapeutic response of 5-FU against GC by targeting CSCs via AKT/GSK-3ß/ß-catenin signaling axis. Our findings offer a crucial insight into the molecular mechanism of Cinobufacini's anticancer activity in GC.

An injectable and self-healing cellulose nanofiber-reinforced alginate hydrogel for bone repair.

Biomedical materials are in high demand for transplantation in cases of diseased or damaged bone tissue. Hydrogels are potential candidates for bone defect repair; however, traditional hydrogels lack the necessary strength and multiple functions. Herein, we effectively synthesized a cellulose nanofiber (CNF)-reinforced oxidized alginate (OSA)/gelatin (Gel) semi-interpenetrating network hydrogel through a facile one-step approach without a cross-linker by using the synergistic effects of dynamic imine bonds and hydrogen bonds. The OSA/Gel/CNF sample showed a notable compressive modulus (up to 361.3 KPa). The gelation time (~150 s) ensured excellent injectability. Self-healing exhibited a high efficiency of up to 92 %, which would enable minimally invasive, dynamic adjustments and personalized therapies. Furthermore, the OSA/Gel/CNF hydrogel showed excellent biomineralization (Ca/P ratio ~ 1.69) and enhanced preosteoblast cell (MC3T3-E1) viability (over 96 %), proliferation, and osteogenic differentiation. Thus, this multifunctional hydrogel has promising potential for using in the bone tissue repairs.

Template-directed growth of sustainable carboxymethyl cellulose-based aerogels decorated with ZIF-67 for activation peroxymonosulfate degradation of organic dyes.

A novel 3D advanced oxidation catalyst ZIF-67@C-CMC/rGO based on carboxymethyl cellulose (CMC) and reduced graphene oxide (rGO) was successfully synthesized by facile in-situ growth of Zeolitic imidazolate framework-67 (ZIF-67). C-CMC/rGO aerogel crosslinked by poly (methyl vinyl ether-alt-maleic acid)/polyethylene glycol system (PMVEMA/PEG) as the host material was prepared through a template-directed growth model and exhibited outstanding mechanical properties. The sustainable composite was successfully used as an efficient catalyst for activating peroxymonosulfate (PMS) to generate SO4-· and ·OH, then leads to the removal of organic contaminants. As a result, almost 100 % of 10 ppm MB/RhB solution can be degraded within 5 min due to the combination of catalyst aerogel and PMS. What's more, the aerogel showed a wide pH tolerance range from 4 to 9 and maintained up to 93 % of the contaminant removal rate compared to the initial value after four cycles. The ZIF-67@C-CMC/rGO aerogel with high load rate and excellent catalytic degradation performance not only solved the problem of dispersion and recovery of ZIF-67 particles, but also provided a new idea for the compound wastewater purification in sulfate radical-based advanced oxidation processes (SR-AOPs).

Injectable multifunctional carboxymethyl chitosan/hyaluronic acid hydrogel for drug delivery systems.

Injectable hydrogels with notable mechanical properties and self-healing ability are promising carriers for use as a drug delivery system. Here, adipic acid dihydrazide (ADH) and calcium ions (Ca2+) were introduced into quaternary ammonium carboxymethyl chitosan and aldehyde-modified hyaluronic acid hydrogels (QCS + OHA). The hydrogels were synthesized through the interaction of the Schiff bases (imine bonds, acylhydrazone bonds) and coordination bonds via a facile one-step approach. The gelation time (∼54 s) ensured excellent injectability. The QCS + OHA + ADH + Ca2+ hydrogel had notable mechanical properties (compressive stress up to 896.30 KPa), good self-healing ability (up to 94 %), good pH responsiveness, and excellent antibacterial properties. In addition, the QCS + OHA + ADH + Ca2+ hydrogel had a high drug loading capacity (121.3 mg/g) and sustained drug release behaviour (≥120 h). The results of cytotoxicity tests showed a high cell proliferation rate (up to 98 %) and good cytocompatibility. In summary, this work presents an injectable and self-healing pH-responsive hydrogel that can be used as a carrier for drug delivery systems.

A high-performance and flexible electrode film based on bacterial cellulose/polypyrrole/nitrogen-doped graphene for supercapacitors.

With the development and popularity of portable electronic devices, there is an urgent need for flexible energy storage devices suitable for mass production. We report freestanding paper electrodes for supercapacitors fabricated via a simple but efficient two-step method. Nitrogen-doped graphene (N-rGO) was first prepared via a hydrothermal method. This not only obtained nitrogen atom-doped nanoparticles but also formed reduced graphene oxide. Pyrrole (Py) was then deposited on the bacterial cellulose (BC) fibers as a polypyrrole (PPy) pseudo-capacitance conductive layer by in situ polymerization and filtered with nitrogen-doped graphene to prepare a self-standing flexible paper electrode with a controllable thickness. The synthesized BC/PPy/N15-rGO paper electrode has a remarkable mass specific capacitance of 441.9 F g-1, a long cycle life (96 % retention after 3000 cycles), and excellent rate performance. The BC/PPy/N15-rGO-based symmetric supercapacitor shows a high volumetric specific capacitance of 244 F cm-3 and a max energy density of 67.9 mWh cm-3 with a power density of 1.48 W cm-3, suggesting that they will be promising materials for flexible supercapacitors.

Multifunctional composite films based on polyvinyl alcohol, quaternary ammonium salt modified cellulose nanofibers and tannic acid-iron ion coordination complexes for food packaging.

The development of sustainable and well-performing food packaging materials takes on critical significance, whereas it is still challenging. To overcome the shortcomings of polyvinyl alcohol (PVA) as a degradable packaging material, in this work, hydrophobic quaternary ammonium salt (QAS) modified cellulose nanofibers (CNF) and tannic acid­iron ion coordination complexes (TA-Fe) were adopted for the preparation of functional PVA films. The modified CNF (CNF-QAS) not only improved the mechanical properties and water resistance of PVA, but also endowed it with antibacterial ability. In addition, the synergistic antibacterial capability with CNF-QAS was achieved using TA-Fe with photothermal therapy. As a result, the modulus, elongation at break, tensile strength, and water contact angle of the prepared PVA films were examined as 88 MPa, 200 %, 11.7 MPa, and 94.8°, respectively. Furthermore, with the assistance of CNF-QAS and TA-Fe, the films inhibited the growth of E. coli and S. aureus by 99.8 % and 99.7 %, respectively, and they exhibited high cell viability of 90.5 % for L929 fibroblasts. Based on the above encouraging properties, the functional PVA films could significantly extend the shelf life of oranges for over two weeks, proving the excellent application prospects in the food packaging field.

Eco-friendly cellulose-based hydrogel functionalized by NIR-responsive multimodal antibacterial polymeric ionic liquid as platform for promoting wound healing.

With the trend of sustainable development and the complex medical environment, there is a strong demand for multimodal antibacterial cellulose wound dressing (MACD) with photothermal therapy (PTT). Herein, a novel MACD fabrication strategy with PTT was proposed and implemented through graft polymerization of an imidazolium ionic liquid monomer containing iron complex anion structure. The fabricated hydrogels exhibited excellent antibacterial properties because of the efficient photothermal conversion ability (68.67 %) of ionic liquids and the intrinsic structural characteristic of quaternary ammonium salts. The antibacterial ratio of cellulosic hydrogel dressings to S. aureus and E. coli could reach 99.57 % and 99.16 %, respectively. Additionally, the fabricated hydrogels demonstrated extremely low hemolysis rates (<5 %) and excellent cell viability (~>85 %). Furthermore, in vivo antibacterial experimental results proved that the fabricated antibacterial dressings could significantly accelerate wound healing. Therefore, the proposed strategy would provide a new method of designing and preparing high-performance cellulose wound dressings.

Biomass-Derived Carbon Dots and Their Sensing Applications.

Carbon dots (CDs) can be widely used in the field of sensing because of its good water solubility, low toxicity, high fluorescence stability and excellent biocompatibility. It has become a popular trend to prepare high-value, inexpensive, renewable and environmentally friendly CDs sensors from biomass resources. This article reviewed the research progress of biomass-derived CDs as chemical, physical and biological sensors in recent years and studied their preparation processes and sensing abilities. Furthermore, the prospects and challenges of biomass-CDs sensors were discussed. This article is expected to provide inspirations for the design, preparation and application of biomass-CDs sensors in the future.

Fabrication of efficient protein imprinted materials based on pearl necklace-like MOFs bacterial cellulose composites.

The acquisition of efficient protein isolation substances is vital for proteomic research, whereas it's still challenging nowadays. Herein, an elaborately designed protein imprinted material based on a bacterial cellulose@ZIF-67 composite carrier (BC@ZIF-67) is proposed for the first time. In particular, due to the ultrafine fiber diameter and abundant hydroxyl functional groups of the bacterial cellulose, BC@ZIF-67 presented a compact arrangement structure similar to a pearl necklace, which greatly promoted template immobilization and mass transfer resistance in protein imprinting technology. Therefore, the protein-imprinted material (BC@ZIF-67@MIPs) fabricated by surface imprinting technology and template immobilization strategy could exhibit ultrahigh adsorption capacity (1017.0 mg g-1), excellent recognition (IF = 5.98) and rapid adsorption equilibrium time (50 min). In addition, based on the experiment outcomes, our team employed BC@ZIF-67@MIPs to enrich template protein in blended protein solutions and biosamples, identifying them as underlying candidates for isolating and purifying proteins.

Temperature/pH-Responsive Carboxymethyl Cellulose/Poly (N-isopropyl acrylamide) Interpenetrating Polymer Network Aerogels for Drug Delivery Systems.

Temperature/pH-responsive carboxymethyl cellulose/poly (N-isopropyl acrylamide) interpenetrating polymer network (IPN) aerogels (CMC/Ca2+/PNIPAM aerogels) were developed as a novel drug delivery system. The aerogel has a highly open network structure with a porosity of more than 90%, which provides convenient conditions for drug release. The morphology and structure of the CMC/Ca2+/PNIPAM aerogels were characterized via scanning electron microscopy (SEM), Micro-CT, X-ray photoelectron spectroscopy (XPS), pore size analysis, and cytotoxicity analysis. The analysis results demonstrate that the aerogel is non-toxic and has more active sites, temperatures, and pH response performances. The anticancer drug 5-fluorouracil (5-FU) was successfully loaded into aerogels through physical entrapment and hydrogen bonding. The drug loading and sustained-release model of aerogels are used to fit the drug loading and sustained-release curve, revealing the drug loading and sustained-release mechanism, and providing a theoretical basis for the efficient drug loading and sustained release.