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Beauveria bassiana is an important entomopathogenic fungus used to control a variety of insect pests. Conidia are the infective propagules of the fungus. However, some important factors that influence conidiation are still to be investigated. In this study, a mutant with decreased conidial production and hyphal growth was identified from a random T-DNA insertional library of B. bassiana. The corresponding gene (Bbthi) for this mutation encodes a putative thiazole synthase. Thiazole and pyrimidine are structural components of thiamine (vitamin B1), which is an essential nutrient for all forms of life. Disruption of Bbthi, Bbpyr, a putative pyrimidine synthetic gene, or both in B. bassiana results in a significant decrease of thiamine content. Loss of Bbthi and Bbpyr function significantly decreased the conidial production and hyphal growth, as well as disrupted the integrity of conidial cell wall. However, the defect of Bbpyr and Bbthi does not decrease the virulence of B. bassiana. Our results indicate the importance of thiamine biosynthesis in conidiation of B. bassiana, and provide useful information to produce conidia of entomopathogenic fungi for biocontrol of insect pests.
Asunto(s)
Beauveria/genética , Proteínas Fúngicas/genética , Genes Fúngicos/fisiología , Esporas Fúngicas/fisiología , Tiamina/biosíntesis , Beauveria/metabolismo , Pared Celular/fisiología , Proteínas Fúngicas/metabolismo , Tiamina/genéticaRESUMEN
Planting densities and nitrogen fertilizer application rates determine the yield of crops. Tartary buckwheat is a pseudocereal crop with great health care and development values. However, little is known about application of nitrogen fertilizer and planting density on the physiological characteristics of Tartary buckwheat. This study aims to clarify the effect of planting density on the senescence and yield of Tartary buckwheat under low nitrogen conditions. A 2-year field experiment was conducted on Tartary buckwheat (Jinqiao 2) to study the effects of different planting densities (8 × 105, 10 × 105, 12 × 105, 14 × 105, and 16 × 105 plants·ha-1) on the root morphology and activity, chlorophyll and malondialdehyde (MDA) contents, antioxidant enzyme activity, photosynthetic characteristics, agronomic traits, and yield of Tartary buckwheat in the absence of nitrogen fertilizer treatment. With the increase in planting density, the root morphological indices and activities; chlorophyll a, chlorophyll b, and carotenoid contents; superoxide dismutase and peroxidase activities; net photosynthetic rate; transpiration rate; intercellular CO2 concentration and transpiration rate; main stem node, branch, and leaf numbers; grain number and weight per plant; and 1000-grain weight of Jinqiao 2 decreased continuously, whereas plant height and leaf MDA content increased continuously. The yield of Tartary buckwheat first increased and then decreased with the increase in planting density. The yield under 14 × 105 plants·ha-1 treatment increased by 68.61%, 44.82%, 11.00%, and 22.36%, respectively, relative to that under 8 × 105, 10 × 105, 12 × 105, and 16 × 105 plants·ha-1treatments. In summary, planting at an appropriately high density (14 × 105 plants·ha-1) can promote the increase in the yield of Tartary buckwheat populations under low nitrogen conditions and is recommended for use in production to achieve the high-yielding and nitrogen saving cultivation of Tartary buckwheat. This research can serve as a theoretical basis to jointly achieve the high yield and nitrogen saving of Tartary buckwheat.
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Background: Tumor radiotherapy combined with immunotherapy for solid tumors has been proposed, but tumor vascular structure abnormalities and immune microenvironment often affect the therapeutic effect of tumor, and multimodal imaging technology can provide more accurate and comprehensive information in tumor research. The purpose of this study was to evaluate the dynamic monitoring of tumor blood vessels and microenvironment induced by radiotherapy by magnetic resonance/photoacoustic (MR/PA) imaging, and to explore its application value in radiotherapy combined with immunotherapy. Methods: The tumor-bearing mice were randomly allocated into six groups, which received different doses of radiation therapy (2 Gy ×14 or 8 Gy ×3) and anti-programmed death ligand-1 (PD-L1) antibody for two consecutive weeks. MR/PA imaging was used to noninvasively evaluate the response of tumor to different doses of radiotherapy, combined with histopathological techniques to observe the tumor vessels and microenvironment. Results: The inhibitory effect of high-dose radiotherapy on tumors was significantly greater than that of low-dose radiotherapy, with the MR images revealing that the signal intensity decreased significantly (P<0.05). Compared with those in the other groups, the tumor vascular density decreased significantly (P<0.01), and the vascular maturity index increased significantly in the low-dose group (P<0.05). The PA images showed that the deoxyhemoglobin and total hemoglobin levels decreased and the SO2 level increased after radiation treatment (P<0.05). In addition, the high-dose group had an increased number of tumor-infiltrating lymphocytes (CD4+ T and CD8+ T cells) (P<0.01, P<0.05) and natural killer cells (P<0.001) and increased PD-L1 expression in the tumors (P<0.05). The combination of radiotherapy and immunotherapy increased the survival rate of the mice (P<0.05), and a regimen of an 8 Gy dose of radiation combined with immunotherapy inhibited tumor growth and increased the survival rate of the mice to a greater degree than the 2 Gy radiation dose with immunotherapy combination (P=0.002). Conclusions: Differential fractionation radiotherapy doses exert biological effects on tumor vascular and the immune microenvironment, and MR/PA can be used to evaluate tumor vascular remodeling after radiotherapy, which has certain value for the clinical applications of radiotherapy combined with immunotherapy.
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Separation of vanadium from molybdates is an essential task for processing the leaching solution of hazardous spent hydrodesulphurization (HDS) catalyst. In this study, the difference in the main naturally occurring mineral forms of Mo and V inspired us to develop a method for the deep removal of V from molybdate solution using Fe3O4 as an adsorbent. First, the adsorbent was synthesized with coprecipitation method, and then it was characterized by XRD, TEM, and VSM. The synthesized material consisted of pure Fe3O4 nanoparticles that exhibited paramagnetic property, with a saturated magnetization of 68.6emug-1. The V removal efficiency was investigated using batch adsorption experiments in varying conditions. Results indicated that V could be deeply removed from various concentrations of molybdate solution at pH of 7.0-11.0 within 5min. A slight decrease was found in the adsorption ratio after the adsorbent had been reused for 4 cycles. The resulting molybdate solution contained less than 0.02gL-1 of V, which satisfies the requirement for preparing high-quality products. Finally, a process flowchart is presented for the separation of Mo and V from the leaching solution of spent HDS catalyst, based on the excellent V removal performance and rapid separation rate of the Fe3O4 adsorbent.