[Realization and Clinical Study of Robotic Technology in Cardiovascular Interventional Surgery].

The high incidence of cardiovascular diseases is a serious threat to human health, and endovascular surgery has become the standard treatment for most interventional cardiovascular diseases. The robotassisted endovascular surgery system further enhances surgeons' ability to perform minimally invasive endovascular procedures in interventional cardiology. This study presents a new robotic technique for coronary intervention from the perspective of clinical application. Aiming at clinical application scenarios, this scheme proposed an intuitive guide wire catheter mechanism design, which accurately and perfectly simulates the doctor's hand movements, realizes the positive and negative direction translation of the guide wire catheter, accurate torque control of the guide wire rotation and locking. The results of animal test showed that the R-OneTM has a high degree of dexterity, accuracy and stability,and meets the clinical needs.

FoxO4 is the main forkhead transcriptional factor localized in the gastrointestinal tracts of pigs.

Forkhead box (Fox) proteins play critical roles in the regulation of differentiation, proliferation, immunity and aging of cells. Most studies on Fox proteins are limited to cultured cells and rodent. The aim of the current study is to detect by immunohistrochemistry whether FoxO1, FoxO3a and FoxO4 proteins are localized in the stomach and intestine of the pig. The results showed that FoxO4 exists in the mucosa in all parts of the stomach and intestine; FoxO3a exists mainly in the lamina propria and muscularis of some parts. However, FoxO1 is not detectable in all parts of the stomach and intestine. Collectively, the results of the present study indicate that there exists a distinct expression pattern of Fox proteins, and that FoxO4 is a primary forkhead transcriptional factor localized in the gastrointestinal tracts of the pig.

[Oral acrylamide affects the development and reproductive performance of male rats].

OBJECTIVE: The content of acrylamide increases remarkably in fried, baked and heat-processed starchy foods. The present experiment was aimed at investigating the toxicity of acrylamide on the reproductive system in male rats. METHODS: Thirty weaned 21-day-old SD male rats were randomly allotted into three groups of 10 each. Group I and Group II were fed on water solutions containing acrylamide 5 mg/kg/d and 10 mg/kg/d for eight consecutive weeks, while the third group on fresh water only as the control. The body weight, viscera weights and testicle and epididymis tissues were detected at the fourth and eighth week respectively. In the end of the experiment, the sperm reserve and morphology in the cauda of the epididymis were examined. RESULTS: The growth of the rats treated with acrylamide was retarded (P < 0.05). The weights of the testis and epididymis and the sperm concentration in the cauda of the epididymis of Group II were decreased significantly (P < 0.05) after acrylamide administration, while no significant change was observed in the sperm concentration of Group I (P > 0.05). Furthermore, histopathological lesions were presented in the testes of the treated rats, and the number of Leydig cells around the apoptosis seminiferous tubules increased significantly (P < 0.05). CONCLUSION: Acrylamide has toxic effects on seminiferous tubules and decreases the production of sperm in male rats.

Age-dependent expression of forkhead box O proteins in the duodenum of rats.

The O subfamily of forkhead box (FoxO) proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B (IGF-1/PI3K/PKB) signal pathway. The objective of the present study was to examine the expressions of three members of FoxO proteins, FoxO1, FoxO3a, and FoxO4 in the duodenum of Sprague-Dawley rats at different ages. The result demonstrated that the expression of FoxO4 in rat duodenum showed an age-dependent manner. At Day 21, there were no detectable localization and expression of FoxO4 in the duodenum, while, at Months 2 and 6, localization and expression of FoxO4 were distinct. In addition, FoxO4 staining was primarily concentrated in the cell nuclei of the lamina propria around the intestinal gland of the duodenum in 2-month-old rats, but was not detectable in the same area in 6-month-old rats. Our results showed also that although FoxO3a existed in the cytoplasm of the lamina propria at a low level at the 2- and 6-month marks, it was still not detectable at Day 21. Besides, FoxO1 was not detectable in all parts and stages. Taken together, our findings suggested that the cell-specific and age-dependent expressional patterns of FoxO4 and FoxO3a proteins in the duodenum play some roles in the development and growth performance of the rat duodenum.