RESUMEN
Objective: To explore the reentry rate of reactive blood donors in the bloodborne pathogen infection screening in Hangzhou City, and analyze the donation behavior of those who successfully returned. Methods: A retrospective analysis of the return data of blood donors with reactive bloodborne pathogen screening markers was conducted at Zhejiang Provincial Blood Center from June 2017 to May 2022. The reentry process for blood donors with reactive bloodborne pathogen screening markers in Hangzhou City is as follows: after the initial screening period of 6 months, donors can voluntarily apply for return to the blood center. Samples are collected and subjected to routine enzyme-linked immunosorbent assay (ELISA) screening for HBsAg, anti-HCV, HIV Ab/Ag, and anti-TP, as well as a single nucleic acid (HIV/HCV/HBV) test. For samples that show non-reactivity in both ELISA and nucleic acid tests, serum biomarker testing for the reasons of exclusion is performed using chemiluminescence immunoassay (CLIA), and those with non-reactivity are allowed to return. Results: A total of 4 583 reactive blood donors who met the criteria for re-entry applied for reentry, out of which 475 applications were received from donors in the Hangzhou area. Among these, 279 donors were successfully readmitted, resulting in a success rate of 58.74% (279/475). By the end of December 2021, out of the 174 donors who successfully returned, 114 donors chose to donate again. They collectively donated 39 530 ml of whole blood and 1 147.2 therapeutic doses of platelets. Among these, 21 donors once again showed reactivity for pathogen infection biomarkers, accounting for 18.42% (21/114). Conclusion: The reentry strategy has somewhat mitigated the attrition of blood donors. Nevertheless, there are instances where donors who were successfully readmitted show reactivity once more in the screening for pathogen infection biomarkers.
Asunto(s)
Infecciones por VIH , Ácidos Nucleicos , Humanos , Donantes de Sangre , Patógenos Transmitidos por la Sangre , Estudios Retrospectivos , Tamizaje Masivo/métodos , Biomarcadores , Virus de la Hepatitis BRESUMEN
A novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction-Sanger sequence-based typing (PCR-SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA-B*40:298:02 differs from HLA-B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (TâC) in exon 3, which corresponds to codon 99 of the mature HLA-B mRNA molecule. This new allele has an estimated frequency of 0.0002, in about 2,500 sequence-based typed subjects from the same population.
Asunto(s)
Alelos , Variación Genética , Antígenos HLA-B/genética , Secuencia de Aminoácidos , Clonación Molecular , Codón , Exones , Antígenos HLA-B/química , Humanos , Masculino , Persona de Mediana Edad , Análisis de Secuencia de ADNRESUMEN
A novel HLA-B*39:01:01-related variant, HLA-B*39:130, has been identified in a normal individual of Han ethnicity in Hunan province, southern China. Following Sanger polymerase chain reaction-sequence-based typing (PCR-SBT), this new allele was further confirmed by cloning, phasing and sequencing. Aligned with HLA-B*39:01:01, HLA-B*39:130 has a nonsynonymous thymine substitution at nucleotide position 94 in exon 4, resulting in amino acid change from threonine to isoleucine at codon 214 (ACAâATA) of the mature HLA-BmRNA molecule.
Asunto(s)
Alelos , Antígenos HLA-B/genética , Adulto , Secuencia de Bases , Clonación Molecular , Humanos , MasculinoRESUMEN
A new MICA allelic variant, MICA*007:07, was identified in an individual of Mongol ethnicity in the Inner Mongolia Autonomous Region, northern China. Following polymerase chain reaction-sequence-based typing (PCR-SBT), this new allele was further confirmed by cloning and sequencing. MICA*007:07 differs from MICA*007:01 by a synonymous mutation from G to A at the 2nd nucleotide position in exon 2. MICA*007:07 was linked to HLA-B*27:05.
Asunto(s)
Secuencia de Bases/genética , Antígenos HLA-B/genética , Antígenos de Histocompatibilidad Clase I/genética , Clonación Molecular , Exones , Femenino , Antígenos HLA-B/inmunología , Antígenos de Histocompatibilidad Clase I/inmunología , Humanos , Desequilibrio de LigamientoRESUMEN
In this study, polymorphisms of major histocompatibility complex class I chain-related genes A and B (MICA and MICB) and human leucocyte antigen (HLA)-B gene were investigated for 158 unrelated Chinese Mongolian subjects recruited from central Inner Mongolia Autonomous Region, northern China, by polymerase chain reaction-sequence-based typing (PCR-SBT) and cloning. Collectively, 79 alleles, including 20 MICA, 12 MICB and 47 HLA-B alleles, were identified. MICA*008:01 (21.2%), MICB*005:02 (48.1%) and HLA-B*51:01 (7.91%) were the most common alleles. Significant global linkage disequilibrium (LD) was detected between HLA-B and MICA, HLA-B and MICB, and MICA and MICB loci (all P < 0.000001). The most frequent haplotypes were HLA-B*51:01-MICA*009:01 (7.28%), HLA-B*58:01-MICB*008 (6.96%), MICA*010-MICB*005:02 (13.92%) and HLA-B*58:01-MICA*002:01-MICB*008 (6.96%). HLA-B-MICA haplotypes such as HLA-B*50:01-MICA*009:02 were associated with single MICB allele. Some HLA-B-MICA haplotypes were associated with multiple MICB alleles, including HLA-B*51:01-MICA*009:01. One novel MICB allele, MICB*031, was identified, which has possibly arisen from MICB*002:01 through single mutation event. We also confirmed the existence of a recently recognized MICA allele, MICA*073, whose ethnic origin has not been previously described. Genotype distributions at MICA, MICB and HLA-B were consistent with a neutrality model. Our results provide new insight into MIC genetic polymorphisms in Chinese ethnic groups. Findings shown here are important from an anthropologic perspective and will inform future studies of the potential role of MIC genes in allogeneic organ transplantation and HLA-linked disease association in populations of related ancestry.
Asunto(s)
Pueblo Asiatico/genética , Antígenos HLA-B/genética , Antígenos de Histocompatibilidad Clase I/genética , Polimorfismo Genético , Alelos , China/epidemiología , Frecuencia de los Genes , Genotipo , Haplotipos , Humanos , Inmunidad/genética , Desequilibrio de Ligamiento , Mongolia/etnología , Mutación/genética , Trasplante de Órganos , Trasplante HomólogoRESUMEN
A novel HLA-B*40 variant, HLA-B*40:186:02, has been identified by cloning and sequencing in a southern Chinese Han population. Aligned with HLA-B*40:01:01, HLA-B*40:186:02 has a nonsynonymous cytosine mutation at nucleotide position 165 in exon 2, leading to amino acid change from glycine to arginine at codon 56. It differs from HLA-B*40:186:01 by a synonymous change (adenine to cytosine) at position 165 in exon 2.
Asunto(s)
Alelos , Sustitución de Aminoácidos/genética , Antígenos HLA-B/genética , Sustitución de Aminoácidos/inmunología , Pueblo Asiatico , Secuencia de Bases , China , Clonación Molecular , Exones/genética , Exones/inmunología , Antígenos HLA-B/inmunología , Secuenciación de Nucleótidos de Alto Rendimiento , HumanosRESUMEN
A new MICA allelic variant, MICA*012:05, has been identified in a Chinese Mongolian population. Following polymerase chain reaction-sequence-based typing (PCR-SBT), this new allele was further confirmed by cloning and sequencing. MICA*012:05 was linked to an HLA-A*24-C*01-B*55:02-DRB1*09 haplotype. MICA*012:05 differs from MICA*012:01 by a single synonymous C to T substitution at nucleotide position 269 in exon 3.
Asunto(s)
Antígenos de Histocompatibilidad Clase I/genética , Antígenos de Histocompatibilidad Clase I/inmunología , Alelos , Pueblo Asiatico , China , Exones/genética , Haplotipos/genética , Haplotipos/inmunología , Antígenos de Histocompatibilidad Clase I/aislamiento & purificación , HumanosRESUMEN
In this report, we present a novel HLA-A*02:07 allele, HLA-A*02:07:08. HLA-A*02:07:08 was identified in an individual of Han ethnicity in Hunan province, southern China. Following polymerase chain reaction-sequence-based typing (PCR-SBT), this new allele was further confirmed by cloning and sequencing. HLA-A*02:07:08 differs from HLA-A*02:07:01 by a single synonymous C to T substitution at nucleotide position 131 in exon 3.
Asunto(s)
Alelos , Clonación Molecular , Antígenos HLA-A/genética , Análisis de Secuencia de ADN , Pueblo Asiatico , China , Exones , HumanosRESUMEN
HLA-DQB1*02:57 has one base substitution at position 260 T>C in exon 2 from HLA-DQB1*02:01:01.
Asunto(s)
Alelos , Pueblo Asiatico/genética , Cadenas beta de HLA-DQ/genética , Prueba de Histocompatibilidad , Reacción en Cadena de la Polimerasa/métodos , Análisis de Secuencia de ADN , Secuencia de Bases , Exones/genética , Humanos , Alineación de SecuenciaRESUMEN
HLA-C*06:103 shows four nucleotides difference from that of HLA-C*06:02:01:01.
Asunto(s)
Genes MHC Clase I , Antígenos HLA-C/genética , Alelos , Secuencia de Bases , China , Trasplante de Células Madre Hematopoyéticas , Humanos , Leucemia/genética , Datos de Secuencia Molecular , Alineación de Secuencia , Homología de Secuencia de Ácido NucleicoRESUMEN
HLA*02:06:14 differs from HLA-A*02:06:01 by a single nucleotide substitution G > A at position 246.
Asunto(s)
Alelos , Exones , Antígeno HLA-A2/genética , Mutación Puntual , Pueblo Asiatico , Secuencia de Bases , Trasplante de Médula Ósea , Genotipo , Antígeno HLA-A2/inmunología , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Análisis de Secuencia de ADN , Donantes de TejidosRESUMEN
HLA-B*27:105 has one nucleotide change from HLA-B*27:04:01 at nucleotide position 404 from G to A.
Asunto(s)
Alelos , Antígeno HLA-B27/genética , Mutación Puntual , Donantes de Tejidos , Pueblo Asiatico , Secuencia de Bases , Trasplante de Médula Ósea , Exones , Expresión Génica , Sitios Genéticos , Heterocigoto , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Alineación de SecuenciaRESUMEN
HLA-A*32:67 has a single nucleotide difference at position 286 C>A compared with HLA-A*32:01:01.
Asunto(s)
Genes MHC Clase I , Antígenos HLA-A/genética , Alelos , Sustitución de Aminoácidos , Secuencia de Bases , Médula Ósea , China , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico , Donantes de TejidosRESUMEN
HLA-A*02:07:06 shows 285 A>C and HLA-A*02:426 has 763 G>A change compared with HLA-A*02:07:01.
Asunto(s)
Genes MHC Clase I , Antígenos HLA-A/genética , Alelos , Médula Ósea , China , Humanos , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Donantes de TejidosRESUMEN
HLA-B*46:01:11 has 219 G>A compared with HLA-B*46:01:01, and HLA-B*51:01:39 shows 561 G>A with HLA-B*51:01:01.
Asunto(s)
Alelos , Antígenos HLA-B/genética , Antígeno HLA-B51/genética , Polimorfismo de Nucleótido Simple , Pueblo Asiatico , Secuencia de Bases , Trasplante de Médula Ósea , Exones , Genotipo , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Donantes de TejidosRESUMEN
Compared with HLA-DQB1*03:03:02:01, HLA-DQB1*03:03:08 and DQB1*03:03:13 have 330 G>C and 349 T>C changes, respectively.
Asunto(s)
Alelos , Trasplante de Médula Ósea , Cadenas beta de HLA-DQ/genética , Polimorfismo de Nucleótido Simple , Donantes de Tejidos , Secuencia de Bases , China , Codón , Exones , Expresión Génica , Cadenas beta de HLA-DQ/inmunología , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Alineación de SecuenciaRESUMEN
HLA-DRB1*10:07 shows one nucleotide different from HLA-DRB1*10:01:01 at position 328 in exon 2.
Asunto(s)
Alelos , Sustitución de Aminoácidos , Trasplante de Médula Ósea , Cadenas HLA-DRB1/genética , Polimorfismo de Nucleótido Simple , Donantes de Tejidos , Secuencia de Bases , China , Codón , Exones , Expresión Génica , Cadenas HLA-DRB1/inmunología , Prueba de Histocompatibilidad , Humanos , Datos de Secuencia Molecular , Alineación de SecuenciaRESUMEN
In this study, we typed 930 cases of nasopharyngeal carcinoma (NPC) and 1134 normal controls recruited from Hunan province, southern China for human leukocyte antigen-A (HLA-A) locus by sequencing exons 2-4. Very significant associations between HLA-A*02:07, HLA-A*11:01 and NPC were established [25.7% vs 16.18%; odds ratio, OR (95% confidence interval, CI) = 1.79 (1.54-2.09), P < 0.0001 and 21.1% vs 30.42%, OR (95% CI) = 0.61 (0.53-0.70), P<0.0001, respectively]. Further analysis of the molecular basis underlying these associations suggests that cysteine (C) at codon 99 of α2-helix of HLA-A protein is probably deleterious and confers risk to NPC. Convincing evidence was uncovered for negative association of a rare allele in southern Chinese populations, HLA-A*31:01, with NPC [0.22% vs 2.12%, OR (95% CI) = 0.1 (0.04-0.28), P < 0.0001]. rs1059449-A, which encodes arginine (R) at codon 56 of α1-helix of HLA-A protein, was postulated to be crucial for such a pattern of negative association with NPC. A subset of NPC cases (N = 632) and normal controls (N=712) were tested for anti-virus capsid antigen (anti-VCA) immunoglobulin A (IgA), very significant difference in seropositivity for anti-VCA IgA was observed between the two groups [67.56% vs 6.46%, OR (95% CI) = 30.16 (21.42-42.46), P < 0.0001]. However, seropositivity for anti-VCA IgA did not correlate with HLA-A allelic typing in both groups.
Asunto(s)
Anticuerpos Antivirales/sangre , Antígenos HLA-A/genética , Inmunoglobulina A/sangre , Neoplasias Nasofaríngeas/genética , Polimorfismo de Nucleótido Simple , Adulto , Alelos , Antígenos Virales/sangre , Proteínas de la Cápside/sangre , Carcinoma , Estudios de Casos y Controles , China , Codón , Exones , Femenino , Frecuencia de los Genes , Antígenos HLA-A/inmunología , Humanos , Masculino , Persona de Mediana Edad , Tipificación Molecular , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/etnología , Neoplasias Nasofaríngeas/inmunología , Neoplasias Nasofaríngeas/patología , Estructura Secundaria de Proteína , Riesgo , Análisis de Secuencia de ADNRESUMEN
Allelic polymorphism and expression variation of killer cell immunoglobulin-like receptor (KIR) 3DL1 on natural killer (NK) cells differ among populations. To determine whether the phenotypic variants are due to KIR polymorphism, transcription or copy number, the allelic polymorphism, mRNA levels and antigen expression of KIR3DL1 were assessed in 162 individuals. We characterized 13 KIR3DL1 alleles, five of which were novel. In addition, 21 genotypes were identified. The correlation between the binding patterns of NK cells to anti-KIR3DL1 and KIR3DL1 alleles was also examined. NK cells with different 3DL1 alleles showed distinct binding levels to anti-KIR3DL1. The binding frequencies of NK cells to anti-KIR3DL1 were not accordant with their binding levels, but both associated with the allele copy numbers. The mRNA expression amounts of individuals with two copy alleles were higher than those of individuals with one copy allele. Our data indicate that both the allele copy number and polymorphism of KIR3DL1 influence the antigen expression on the NK-cell surface, but only the copy number was associated with mRNA expression.
Asunto(s)
Pueblo Asiatico/genética , Variación Genética , Receptores KIR3DL1/genética , Alelos , Expresión Génica , Frecuencia de los Genes , Humanos , Células Asesinas Naturales/inmunología , Polimorfismo Genético , Receptores KIR3DL1/metabolismo , Receptores KIR3DS1/genéticaRESUMEN
HLA-B*39:01:01:03 allele differs from HLA-B*39:01:01:01 allele by a single nucleotide substitution.