SADS-CoV nsp1 inhibits the STAT1 phosphorylation by promoting K11/K48-linked polyubiquitination of JAK1 and blocks the STAT1 acetylation by degrading CBP.

The newly discovered zoonotic coronavirus swine acute diarrhea syndrome coronavirus (SADS-CoV) causes acute diarrhea, vomiting, dehydration, and high mortality rates in newborn piglets. Although SADS-CoV uses different strategies to evade the host's innate immune system, the specific mechanism(s) by which it blocks the interferon (IFN) response remains unidentified. In this study, the potential of SADS-CoV nonstructural proteins (nsp) to inhibit the IFN response was detected. The results determined that nsp1 was a potent antagonist of IFN response. SADS-CoV nsp1 efficiently inhibited signal transducer and activator of transcription 1 (STAT1) phosphorylation by inducing Janus kinase 1 (JAK1) degradation. Subsequent research revealed that nsp1 induced JAK1 polyubiquitination through K11 and K48 linkages, leading to JAK1 degradation via the ubiquitin-proteasome pathway. Furthermore, SADS-CoV nsp1 induced CREB-binding protein degradation to inhibit IFN-stimulated gene production and STAT1 acetylation, thereby inhibiting STAT1 dephosphorylation and blocking STAT1 transport out of the nucleus to receive antiviral signaling. In summary, the results revealed the novel mechanisms by which SADS-CoV nsp1 blocks the JAK-STAT signaling pathway via the ubiquitin-proteasome pathway. This study yielded valuable findings on the specific mechanism of coronavirus nsp1 in inhibiting the JAK-STAT signaling pathway and the strategies of SADS-CoV in evading the host's innate immune system.

Helicobacter hepaticus promotes liver fibrosis through oxidative stress induced by hydrogenase in BALB/c mice.

BACKGROUND: It has been documented that Helicobacter hepaticus produces a nickel-containing hydrogen-oxidizing hydrogenase enzyme, which is necessary for hydrogen-supported amino acid uptake. Although H. hepaticus infection has been shown to promote liver inflammation and fibrosis in BALB/c mice, the impact of hydrogenase on the progression of liver fibrosis induced by H. hepaticus has not been explored. MATERIALS AND METHODS: BALB/c mice were inoculated with hydrogenase mutant (ΔHyaB) or wild type (WT) H. hepaticus 3B1 for 12 and 24 weeks. H. hepaticus colonization, hepatic histopathology, serum biochemistry, expression of inflammatory cytokines, and oxidative stress signaling pathways were detected. RESULTS: We found that ΔHyaB had no influence on the colonization of H. hepaticus in the liver of mice at 12 and 24 weeks post infection (WPI). However, mice infected by ΔHyaB strains developed significantly alleviated liver inflammation and fibrosis compared with WT infection. Moreover, ΔHyaB infection remarkably increased the expression of hepatic GSH, SOD, and GSH-Px, and decreased the liver levels of MDA, ALT, and AST compared to WT H. hepaticus infected group from 12 to 24 WPI. Furthermore, mRNA levels of Il-6, Tnf-α, iNos, Hmox-1, and α-SMA were significantly decreased with an increase of Nfe2l2 in the liver of mice infected by ΔHyaB strains. In addition, ΔHyaB H. hepaticus restored the activation of the Nrf2/HO-1 signaling pathway, which is inhibited by H. hepaticus infection. CONCLUSIONS: These data demonstrated that H. hepaticus hydrogenase promoted liver inflammation and fibrosis development mediated by oxidative stress in male BALB/c mice.

Musical coordination affects children's perspective-taking, but musical synchrony does not.

Perspective-taking, which is important for communication and social activities, can be cultivated through joint actions, including musical activities in children. We examined how rhythmic activities requiring coordination affect perspective-taking in a referential communication task with 100 Chinese 4- to 6-year-old children. In Study 1, 5- to 6-year-old children played an instrument with a virtual partner in one of three coordination conditions: synchrony, asynchrony, and antiphase synchrony. Eye movements were then monitored with the partner giving instructions to identify a shape referent which included a pre-nominal scalar adjective (e.g., big cubic block). When the target contrast (a small cubic block) was in the shared ground and a competitor contrast was occluded for the partner, participants who used perspective differences could, in principle, identify the intended referent before the shape was named. We hypothesized that asynchronous and antiphase synchronous musical activities, which require self-other distinction, might have stronger effects on perspective-taking than synchronous activity. Children in the asynchrony and antiphase synchrony conditions, but not the synchrony condition, showed anticipatory looks at the target, demonstrating real-time use of the partner's perspective. Study 2 was conducted to determine if asynchrony and antiphase asynchrony resulted in perspective-taking that otherwise would not have been observed, or if synchronous coordination inhibited perspective-taking that would otherwise have occurred. We found no evidence for online perspective-taking in 4- to 6-year-old children without music manipulation. Therefore, playing instruments asynchronously or in alternation, but not synchronously, increases perspective-taking in children of this age, likely by training self-other distinction and control. A video abstract of this article can be viewed at https://youtu.be/TM9h_GpFlsA. RESEARCH HIGHLIGHTS: This study is the first to show that rhythmic coordination, a form of non-linguistic interaction, can affect children's performance in a subsequent linguistic task. Eye-movement data revealed that children's perspective-taking in language processing was facilitated by prior asynchronous and antiphase synchronous musical interactions, but not by synchronous coordination. The results challenge the common "similar is better" view, suggesting that maintaining self-other distinction may benefit social interactions that involve representing individual differences.

Longitudinal development of children's sharing behaviour: Only children versus children with siblings from rural China.

This study examined the development of children's sharing behaviour towards friends and strangers using dictator games with a longitudinal design in a sample of rural Chinese children (n = 589, 47.0% girls) at 3-4 years old and 2 years later (n = 453, 44.2% girls). Results showed that the willingness to share and the amount of sharing changed over time and were affected by family structure. Only children shared fewer stickers than non-only children at ages 3-4, but the amount they shared did not differ at ages 5-6. Only children may develop reciprocal friendships at an older age due to their lack of experience with siblings. Children shared more stickers with friends than strangers at ages 3-4, and such ingroup bias became stronger at ages 5-6.

Group decision-making on risky choice in adolescents and young adults.

Adolescence, a period during which risk-taking behaviors frequently occur, is susceptible to peer influence. However, the direction of peer influence on group decision-making among adolescents and whether it increases group decision-making risk-seeking or risk-aversion is still unclear. This study recruited 84 adolescents (age = 14.44, 48 girls) and 99 young adults (M age = 20.48, 48 women) and adopted two framing tasks (life and money problems) to examine the differences between individual decision-making and group decision-making (of three members each), as well as the strategies for reaching consensus in group discussion. Results showed no evidence that adolescents are more risk seeking than adults in individual decision-making, and the adolescents were even more risk averse toward money problems than adults. We also found that the adolescents were more risk seeking for life problems but more risk averse for money problems in group decision-making than in individual decision-making under the loss frame. Further analysis of group discussion showed that the adolescents were more likely to apply the strategy of "one person puts forward an idea and then the others follow" to reach an agreement, while the adults tended to vote. This study indicated that peers' influence on group decision-making is domain specific, especially among adolescents. Supplementary Information: The online version contains supplementary material available at 10.1007/s12144-022-04027-5.

Power grabbed or granted: Children's allocation of resources in social power situations.

Notwithstanding alternative ways of obtaining power, social power is mostly commonly acquired through either a dominance approach, where power is grabbed by the powerholder, or a prestige approach, where power is granted by group members. Although children's attitude toward power in the dominance situation has been studied, little is known about how children understand and distinguish different ways of obtaining power. We examined the understanding of power in children aged 4-8 years by their resource allocation behavior in two social power acquisition situations. In Study 1, 4- to 8-year-olds (N = 123) gradually shifted from distributing more to the powerholder to showing no preference for either party (in the prestige situation) or to distributing more to the subordinates (in the dominance situation) as they age. Older children (6-8 years), but not 4- and 5-year-olds, were more likely to favor the powerholders in the prestige situation than in the dominance situation. In Study 2, when power did not produce unfair results, 7- and 8-year-olds (N = 48) favored the powerholder in the prestige situation but showed no preference in the dominance situation. The results suggest that children's attitudes toward the two ways of acquiring power are gradually differentiated with age, and children's resource allocation in the power situations is influenced by the way of acquiring power and children's equity concern.

Psychological implications of the COVID-19 pandemic around the world: Introduction to the special issue.

Although psychological researchers have long studied the implications of major crises, the outbreak and spread of the COVID-19 pandemic have confronted the global community of psychologists and psychological researchers with new challenges. This special issue contributes to the growing empirical literature on the immediate psychological implications of the COVID-19 pandemic. We present and discuss diverse work from authors that followed our call for papers in May 2020, shortly after the World Health Organisation declared COVID-19 a global pandemic. The studies focus on the early phases of the pandemic by addressing (a) implications of the pandemic for psychological well-being and mental health, (b) psychological effects of lockdown scenarios as well as (c) individual compliance with COVID-19 prevention and intervention measures. We conclude by highlighting the need for new research efforts, with a special focus on low- and middle-income regions, international research collaborations and cross-cultural research designs.

Helicobacter hepaticus infection-induced IL-33 promotes hepatic inflammation and fibrosis through ST2 signaling pathways in BALB/c mice.

It has been documented that Helicobacter hepaticus (H. hepaticus) infection is linked to hepatic inflammation and fibrosis. Interleukin 33 (IL-33) is a cytokine involved in inflammatory and fibrotic diseases, but its relevance to H. hepaticus infection-induced liver inflammation and fibrosis is unknown. In this study, we found that the expression of IL-33 in mice liver was significantly induced by H. hepaticus infection at 24 weeks post infection (WPI). Immunohistochemistry analysis revealed that IL-33 was transferred from the nucleus to the cytoplasm due to infection. The quantitation of inflammatory cytokine and histopathology evaluation showed that IL-33 knockdown attenuated the H. hepaticus-induced hepatic inflammation and fibrosis. More importantly, H. hepaticus promoted the expression of the IL-33 receptor ST2 on cell surfaces, and the expression of ST2 then activated the expression nuclear factor-κB (p65), α-SMA, and Erk1/2. These observations provide novel insights into the pathogenic mechanism of hepatic inflammation and fibrosis during H. hepaticus infection.

Low frequency noise-dark current correlations in HgCdTe infrared photodetectors.

In this paper, low frequency noise and dark current correlation is investigated as a function of reverse bias and temperature for short-wave infrared (SWIR), mid-wave infrared (MWIR), and long-wave infrared (LWIR) HgCdTe homo-junction photodetectors. Modelling of dark current-voltage characteristics shows that the detectors have ohmic-behavior under small reverse bias, thus enabling further analysis of 1/f noise-current dependences with the empirical square-law relation (SI ∼ I2) at different temperature regions. It is found that for the SWIR and MWIR devices, the total 1/f noise spectral density at arbitrary temperatures can be modelled by the sum of shunt and generation-recombination noise as SI(T,f)=[αSHISH2(T)+αG-RIG-R2(T)]/f, with no contribution from the diffusion component observed. On the other hand, for the LWIR device the diffusion component induced 1/f noise that cannot be overlooked in high temperature regions, and a 1/f noise-current correlation of SI(T,f)={αs[IDIFF2(T)+IG-R2(T)]+αSHISH2(T)}/f is proposed, with a shared noise coefficient of αs ≅ 1 × 10-9 which is close to that calculated for shunt noise. The 1/f noise-current correlation established in this work can provide a powerful tool to study the low frequency noise characteristics in HgCdTe-based photodetectors and to help optimizing the "true" detectivity of devices operating at low frequency regime.

Helicobacter hepaticus infection induces chronic hepatitis and fibrosis in male BALB/c mice via the activation of NF-κB, Stat3, and MAPK signaling pathways.

BACKGROUND: It has been documented that Helicobacter hepaticus (H hepaticus) infection is linked to chronic hepatitis and liver cancer. However, our understanding of the molecular mechanisms underlying progression of the H hepaticus-induced hepatic inflammation to cellular hepatocarcinoma is still limited. MATERIALS AND METHODS: In our study, male BALB/c mice were infected by H hepaticus for 8, 12, 16, 20, and 24 weeks. Histopathology, H hepaticus colonization dynamics, select signaling pathways, and expression of key inflammatory cytokines in the liver were examined. RESULTS: We found that H hepaticus was detectible in feces of mice at 7 days postinfection (DPI) by PCR, but it was not detected in the livers by PCR until 8 weeks postinfection (WPI). In addition, abundance of colonic and hepatic H hepaticus was progressively increased over the infection duration. H hepaticus-induced hepatic inflammation and fibrosis were aggravated over the infection duration, and necrosis or cirrhosis developed in the infected liver at 24 WPI H hepaticus infection increased levels of alanine aminotransferase and aspartate aminotransferase. Moreover, mRNA levels of Il-6 and Tnf-α were significantly elevated in the livers of H hepaticus-infected mice compared to uninfected control from 8 WPI to 24 WPI. Furthermore, Stat3, nuclear factor-κB (p65), and MAPK (Erk1/2 and p38) were activated by H hepaticus infection. CONCLUSIONS: These data demonstrated that male BALB/c mice can be used as a new mouse model of H hepaticus-induced liver diseases and that the H hepaticus-induced liver injury is triggered by NF-κB, Jak-Stat, and MAPK signaling pathways.

Targeting secreted cytokine BMP9 gates the attenuation of hepatic fibrosis.

Liver fibrosis is overly exuberant wound healing that leads to portal hypertension or liver cirrhosis. Recent studies have demonstrated the functions of bone morphogenetic protein 9 (BMP9) in liver fibrosis, and thus, targeting liver-specific BMP9 abnormalities will become an attractive approach for developing therapeutics to treat liver fibrosis. Here, we reveal that BMP9 serves as a valuable serum diagnostic indicator and efficient therapeutic target to attenuate liver fibrogenesis. Our analysis of biopsies from liver fibrotic patients revealed that higher BMP9 levels accompanied advanced stages of liver fibrosis. In mouse models, recombinant Bmp9 overexpression accelerated liver fibrosis, and adenovirus-mediated Bmp9 knockdown attenuated liver fibrogenesis. Intriguingly, BMP9 directly stimulated hepatic stellate cell activation via the SMAD signaling pathway to enhance hepatic fibrosis. Moreover, an inhibitory monoclonal antibody targeting Bmp9 was efficacious in treatment of mice with liver fibrosis. These observations delineate a novel model in which BMP9 directly drives SMAD/ID1 signaling in hepatic stellate cells, which modulates liver fibrogenesis development. Moreover, the findings unveil a promising surrogate biomarker for the diagnosis of hepatic fibrosis, thereby representing an efficient "BMP9 neutralization" approach in alleviating hepatic fibrosis.

Characterization of a conjunctival melanoma cell line CM-AS16, newly-established from a metastatic Han Chinese patient.

Conjunctival melanoma (CM) is associated with metastases formation, can be fatal, and occurs in all different races. While cell lines are essential for experimental research, all available CM cell lines are derived from Caucasian patients. Furthermore, they are not derived from metastases. We aimed to establish a new CM cell line from a parotid metastasis in a Han Chinese patient and to depict its characteristics. The novel cell line, CM-AS16, was obtained from a surgical parotid sample and determined as a unique one with short tandem repeat (STR) analysis. It has been successively sub-cultured in vitro for more than 100 passages and exhibits rapid proliferation and migration. Chromosome analysis shows abundant chromosome aberrations, while whole exome sequencing (WES) reveals a typical NRAS mutation (Q61R). In vivo tumor growth was successfully established in a NOD/SCID mice model, and the immunophenotypes, such as HMB45, Melan A, S100, SOX10 and Ki67, manifested similar between the original tumor and the xenograft by immunohistochemistry. A MEK inhibitor binimetinib prominently suppressed in vitro cell growth by inhibiting ERK1/2 phosphorylation. In addition, monoclonal cells were used to demonstrate the drug sensitivity of different cells. In conclusion, the first cell line, CM-AS16, that is derived from a CM in a Han Chinese patient has highly malignant characteristics and a typical NRAS mutation. It may be used as a tool for further exploration of the molecular mechanisms of CM.

The association between punishment and cooperation in children with high-functioning autism.

This study examined judgment about punishment and whether punishment promoted cooperation in the prisoner's dilemma game (PDG) in children with high-functioning autism (HFA) and typically developing (TD) children. In total, 66 6- to 12-year-olds participated in this study. Children were first asked about judgments regarding rewards and punishment in stories, and then they were asked to play the PDG with a partner in conditions with and without punishment. Results showed that children with HFA believed that hitting others should deserve punishment to a greater extent than TD children did. It indicated that children with HFA understood that bad acts should be punished, suggesting that these children have already acquired the general concept of "punishment." Children displayed higher levels of cooperation in the condition with punishment than in the condition without punishment in the PDG, suggesting that punishment promoted cooperation in the PDG in both children with HFA and TD children.

Parental mind-mindedness but not false belief understanding predicts Hong Kong children's lie-telling behavior in a temptation resistance task.

Children can tell lies before they understand the concept of false belief. This study investigated the relationship between parental mind-mindedness, defined as the propensity of parents to view their children as mental agents with independent thoughts and feelings, and the lie-telling behavior of Hong Kong children aged 3-6years. The results confirmed earlier findings indicating that Hong Kong children's understanding of false belief is delayed; nevertheless, the participants appeared to lie just as well as children from other cultures. The lie-telling behavior of Hong Kong children was predicted by parental mind-mindedness and children's age but was unrelated to children's false belief understanding. It is suggested that children of mind-minded parents are more likely to exercise autonomy in socially ambiguous situations. Future studies should focus on the roles of parenting and children's multifaceted autonomy when addressing children's adaptive lie telling.

Is the bias for function-based explanations culturally universal? Children from China endorse teleological explanations of natural phenomena.

Young children in Western cultures tend to endorse teleological (function-based) explanations broadly across many domains, even when scientifically unwarranted. For instance, in contrast to Western adults, they explicitly endorse the idea that mountains were created for climbing, just like hats were created for warmth. Is this bias a product of culture or a product of universal aspects of human cognition? In two studies, we explored whether adults and children in Mainland China, a highly secular, non-Western culture, show a bias for teleological explanations. When explaining both object properties (Experiment 1) and origins (Experiment 2), we found evidence that they do. Whereas Chinese adults restricted teleological explanations to scientifically warranted cases, Chinese children endorsed them more broadly, extending them across different kinds of natural phenomena. This bias decreased with rising grade level across first, second, and fourth grades. Overall, these data provide evidence that children's bias for teleological explanations is not solely a product of Western Abrahamic cultures. Instead, it extends to other cultures, including the East Asian secular culture of modern-day China. This suggests that the bias for function-based explanations may be driven by universal aspects of human cognition.

Children's Sharing Behavior in Mini-Dictator Games: The Role of In-Group Favoritism and Theory of Mind.

This study investigated the motivational and social-cognitive foundations (i.e., inequality aversion, in-group bias, and theory of mind) that underlie the development of sharing behavior among 3- to 9-year-old Chinese children (N = 122). Each child played two mini-dictator games against an in-group member (friend) and an out-group member (stranger) to divide four stickers. Results indicated that there was a small to moderate age-related increase in children's egalitarian sharing with strangers, whereas the age effect was moderate to large in interactions with friends. Moreover, 3- to 4-year-olds did not treat strangers and friends differently, but 5- to 6-year-old and older children showed strong in-group favoritism. Finally, theory of mind was an essential prerequisite for children's sharing behavior toward strangers, but not a unique predictor of their sharing with friends.

How Universal Are Free Will Beliefs? Cultural Differences in Chinese and U.S. 4- and 6-Year-Olds.

This study explores the development of free will beliefs across cultures. Sixty-seven Chinese 4- and 6-year-olds were asked questions to gauge whether they believed that people could freely choose to inhibit or act against their desires. Responses were compared to those given by the U.S. children in Kushnir, Gopnik, Chernyak, Seiver, and Wellman (). Results indicate that children from both cultures increased the amount of choice they ascribed with age. For inhibition questions, Chinese children ascribed less choice than the U.S. children. Qualitative explanations revealed that the U.S. children were also more likely to endorse notions of autonomous choice. These findings suggest both cultural differences and similarities in free will beliefs.

Predictors of media multitasking in Chinese adolescents.

We examined predictors of media multitasking in Chinese adolescents from 3 contexts: characteristics of the media user, types of media use and family media contexts. Three hundred and twenty adolescents, 11-18 years of age, completed questionnaires to measure media use, impulsivity, sensation seeking, time management disposition and family media environment. The results showed that media multitasking was positively correlated with age and total media use time. Participants with high levels of impulsivity and sensation seeking reported more multitasking behaviour. Multitasking was negatively correlated with time management. Children from media-oriented families often engage in more multitasking. What's more, social networking sites use and music use can mediate the effect of individual and family factors on media multitasking.

Developmental changes in children's understanding of horizontal projectile motion.

This study investigated 5- to 13-year-old children's performance in solving horizontal projectile motion problems, in which they predicted the trajectory of a carried object released from a carrier in three different contexts. The results revealed that 5- and 8-year-olds' trajectory predictions were easily distracted by salient contextual features (e.g. the relative spatial locations between objects), whereas a proportion of 11- and 13-year-olds' performance suggested the engagement of the impetus concept in trajectory prediction. The impetus concept is a typical misconception of inertial motion that assumes that motion is caused by force. Children's performance across ages suggested that their naïve knowledge of projectile motion was neither well-developed and coherent nor completely fragmented. Instead, this study presented the dynamic process in which children with age gradually overcame the influences of contextual features and consistently used the impetus concept across motion problems.

[Impacts of biological and family factors on lexical and intellectual development in Mandarin-speaking children].

OBJECTIVE: To investigate the impacts of biological factors (age and sex) and family factors (socioeconomic status and parenting style) on the early lexical and intellectual development of children in a longitudinal tracking study. METHODS: A total of 38 Mandarin-speaking children aged from 18 to 24 months were surveyed using the Putonghua Chinese Communicative Development Inventory (PCDI), the Ages and Stages Questionnaire (ASQ), and a self-designed Questionnaire for Parents. All of the subjects were retested using PCDI and ASQ after 6 months. RESULTS: Biological factors accounted for 65% of the variance in lexical development, 10% of which was attributed to gender, in the first survey. After six months, the contribution of age decreased to 26% and gender had no significant impact. Lexical development could positively predict the intellectual development of children. When age and gender were controlled, it accounted for 22% of the variance in intellectual development. Family socioeconomic factors had no significant impacts on lexical and intellectual development. Children's recognition of people and objects around them with guidance of parents in parenting styles could positively predict the intellectual development of children six months later, which accounted for 10% of the variance. CONCLUSIONS: Biological factors play an important role in the early lexical development of children. However, the influence decreases with the increase of age (months). Biological factors, lexical development, and parenting style have a combined influence on children's intellectual development.