RESUMEN
Territoriality is costly, and the accurate identification of intruders and the decision to perform aggressive responses are key behavioral traits in social animals. We studied aggression among individuals belonging to close and distant nests of the plant-ant Azteca muelleri, which lives in stems of the pioneer tree Cecropia glaziovii. More specifically, we aim to investigate if the DE (dear-enemy effect-less aggression towards neighbors than strangers) or NN (nasty-neighbor effect-less aggression to strangers than neighbors) effects or even none of them apply for this iconic Azteca-Cecropia system. We further checked if ant aggression towards conspecifics is related to cuticular hydrocarbon profiles (CHCs), which provide chemical cues for nestmate recognition. Therefore, we sampled 46 nests of A. muelleri in three Brazilian Atlantic forest fragments and performed behavioral trials within and between sites. Consistently with the DE effect, we found higher aggression levels in 'between sites' versus 'within sites' treatments as well as a positive effect of spatial distance on ant aggressiveness. We found no effect of the overall dissimilarities on CHC blend on ant aggressiveness, but of one CHC class, the methylated alkanes. Overall, we provide key insights on nest-mate recognition in obligatory ant-plant mutualisms.
RESUMEN
Herbivory is ubiquitous. Despite being a potential driver of plant distribution and performance, herbivory remains largely undocumented. Some early attempts have been made to review, globally, how much leaf area is removed through insect feeding. Kozlov et al., in one of the most comprehensive reviews regarding global patterns of herbivory, have compiled published studies regarding foliar removal and sampled data on global herbivory levels using a standardized protocol. However, in the review by Kozlov et al., only 15 sampling sites, comprising 33 plant species, were evaluated in tropical areas around the globe. In Brazil, which ranks first in terms of plant biodiversity, with a total of 46,097 species, almost half (43%) being endemic, a single data point was sampled, covering only two plant species. In an attempt to increase knowledge regarding herbivory in tropical plant species and to provide the raw data needed to test general hypotheses related to plant-herbivore interactions across large spatial scales, we proposed a joint, collaborative network to evaluate tropical herbivory. This network allowed us to update and expand the data on insect herbivory in tropical and temperate plant species. Our data set, collected with a standardized protocol, covers 45 sampling sites from nine countries and includes leaf herbivory measurements of 57,239 leaves from 209 species of vascular plants belonging to 65 families from tropical and temperate regions. They expand previous data sets by including a total of 32 sampling sites from tropical areas around the globe, comprising 152 species, 146 of them being sampled in Brazil. For temperate areas, it includes 13 sampling sites, comprising 59 species. Thus, when compared to the most recent comprehensive review of insect herbivory (Kozlov et al.), our data set has increased the base of available data for the tropical plants more than 460% (from 33 to 152 species) and the Brazilian sampling was increased 7,300% (from 2 to 146 species). Data on precise levels of herbivory are presented for more than 57,000 leaves worldwide. There are no copyright restrictions. Please cite this paper when using the current data in publications; the authors request to be informed how the data is used in the publications.