Detection of IgG-bearing erythrocytes by a sensitive Staphylococcus aureus protein A-binding radioassay: possible usefulness for the differential diagnosis of connective tissue diseases.

We describe a sensitive radioassay for detecting erythrocyte-associated immunoglobulin employing the immunoglobulin-specific reagent Staphylococcus aureus protein A. The assay was used to determine the extent to which erythrocytes from patients with different connective tissue disease bind radiolabelled protein A. Increased amounts of protein A were bound by erythrocytes from patients with systemic lupus erythematosus or with progressive systemic sclerosis when compared to a control group, whereas there was no significant binding to erythrocytes from patients with rheumatoid arthritis or with polymyositis-dermatomyositis. The assay, because of its high sensitivity and of the availability of the reagent in pure form, should be useful for the investigation of cell-bound antibodies and for the differential diagnosis of connective tissue diseases.

[Satisfaction and unsatisfaction factors in patients assisted by the SUS]. / Fatores de satisfação e insatisfação entre os pacientes assistidos pelo SUS.

This study has the purpose to identify the factors that cause satisfaction and dissatisfaction to the patients who are interns in hospitals which have agreements with "Sistema Unico de Saúde-SUS", based on the assistance rendered during the period of internment. The samples were constituted of 170 patients. To obtain the data, there was used a questionnaire containing 47 closed questions. The results obtained revealed several aspects that compromise the quality of the service rendered, such as: service doctors, nutrition, nurse and the environment. Thus, concludes there is dissatisfaction of the patients with the service offered by SUS.