Cementum and enamel surface mimicry influences soft tissue cell behavior.

AIMS: To test whether titanium surface roughness disparity might be used to specifically guide the behavior of gingiva fibroblasts and keratinocytes, thereby improving the quality of soft tissue (ST) integration around abutments. METHODS: Titanium discs resembling the roughness of enamel (M) or cementum (MA) were created with normal or increased hydrophilicity and used as substrates for human fibroblasts and keratinocytes. Adhesion and proliferation assays were performed to assess cell-type specific responses upon encountering the different surfaces. Additionally, immunofluorescence and qPCR analyses were performed to study more in depth the behavior of fibroblasts and keratinocytes on MA and M surfaces, respectively. RESULTS: While enamel-like M surfaces supported adhesion, growth and a normal differentiation potential of keratinocytes, cementum-emulating MA surfaces specifically impaired the growth of keratinocytes. Vice versa, MA surfaces sustained regular adhesion and proliferation of fibroblasts. Yet, a more intimate adhesion between fibroblasts and titanium was achieved by an increased hydrophilicity of MA surfaces, which was associated with an increased expression of elastin. CONCLUSION: The optimal titanium implant abutment might be achieved by a bimodal roughness design, mimicking the roughness of enamel (M) and cementum with increased hydrophilicity (hMA), respectively. These surfaces can selectively elicit cell responses favoring proper ST barrier by impairing epithelial downgrowth and promoting firm adhesion of fibroblasts.

A survey for the use of torque-limiting devices among dental clinicians in Europe.

PURPOSE: To assess how well torque-limiting devices (TLDs) are known and used by European dentists, and their adherence to screw tightening protocols and screw loosening occurrence through a survey, including the correlation between the dental specialty-of-interest and the recognition, the tightening protocol used, and between the calibration and the occurrence of screw loosening. MATERIALS AND METHODS: A 10-question survey was distributed to dentists to collect data on their specialty-of-interest, TLD usage, knowledge on TLDs, calibration, the term "preload," tightening speed, tightening protocols used, and occurrence of screw loosening. Pearson test was used for correlation analysis between the specialty-of-interest and the recognition-based questions, the tightening protocol used, and between the calibration and the frequency of screw loosening. RESULTS: Of 422 respondents, 24% calibrated their TLDs, 27% knew the term "preload," 76% selected the correct location to read on TLDs, and 6% was aware of the effect of tightening speed. The correlation between the specialty-of-interest and the recognition-based questions was nonsignificant (p < .05) but was significant for used tightening protocol (p < .001). The correlation between the calibration and the occurrence of screw loosening was nonsignificant (p = 0.16). Tightening protocols' effect on screw loosening was similar, which was mostly observed less than once a year (p < .001). CONCLUSIONS: A lack in dentists' knowledge was found on calibration, the term preload, and the effect of tightening speed, which were not impacted by the dentists' specialty-of-interest, which affected the preferred tightening protocol. The tightening protocol and calibration did not impact the occurrence of screw loosening, which was mostly observed less than once a year.

Material and abutment selection for CAD/CAM implant-supported fixed dental prostheses in partially edentulous patients - A narrative review.

Restorative material selection has become increasingly challenging due to the speed of new developments in the field of dental material science. The present narrative review gives an overview of the current indications for implant abutments and restoration materials for provisional and definitive implant-supported fixed dental prostheses in partially edentulous patients. For single implant restorations, titanium base abutments for crowns are suggested as an alternative to the conventional stock- and customized abutments made out of metal or zirconia. They combine the mechanical stability of a metallic connection with the esthetic potential of ceramics. For multiple-unit restorations, conical titanium bases especially designed for bridges are recommended, to compensate for deviating implant insertion axes and angulations. Even though titanium base abutments with different geometries and heights are available, certain clinical scenarios still benefit from customized titanium abutments. Indications for the definitive material in fixed implant restorations depend on the region of tooth replacement. In the posterior (not esthetically critical) zone, ceramics such as zirconia (3-5-Ymol%) and lithium-disilicate are recommended to be used in a monolithic fashion. In the anterior sector, ceramic restorations may be buccally micro-veneered for an optimal esthetic appearance. Lithium-disilicate is only recommended for single-crowns, while zirconia (3-5-Ymol%) is also recommended for multiple-unit and cantilever restorations. Attention must be given to the specific mechanical properties of different types of zirconia, as some feature reduced mechanical strengths and are therefore not indicated for all regions and restoration span lengths. Metal-ceramics remain an option, especially for cantilever restorations.

Effect of laser-microtextured abutments on peri-implant outcomes: a systematic review and meta-analysis.

OBJECTIVES: To evaluate the potential of laser-microtextured abutments (LMAs) compared to machined abutments (MAs) in peri-implant clinical and radiographic outcomes. MATERIALS AND METHODS: Eligible studies consisted of randomized clinical trials (RCTs) retrieved from MEDLINE, Web of Science, Scopus, and Embase databases. The study adhered to the PRISMA statement, and the protocol was registered at the PROSPERO (registration number CRD42023443112). The risk of bias was evaluated according to version 2 of the Cochrane risk of bias tool (RoB 2). Meta-analyses were performed using random effect models. Afterward, the GRADE approach was used to determine the certainty of evidence. RESULTS: Four RCTs were included from a total of 2,876 studies. LMAs had lower peri-implant sulcus depth at 6-8 weeks (WMD: -0.69 mm; 95% CI: -0.97, -0.40; p = 0.15, I2 = 53%) and at one year (WMD: -0.75 mm; 95% CI: -1.41, -0.09; p = 0.09, I2 = 65%), but the certainty of evidence was low. In addition, the marginal bone loss favored the LMAs group (WMD: -0.29 mm; 95% CI: -0.36, -0.21; p = 0.69, I2 = 0%) with moderate evidence. There were fewer sites with bleeding on probing in the LMAs group (WMD: -1.10; 95% CI: -1.43, -0.77; p = 0.88, i2 = 0%). There was no statistical difference between groups for the modified gingival index and modified plaque index. Furthermore, all studies were classified as having some concerns risk of bias. CONCLUSIONS: There was low to moderate certainty evidence that LMAs can favor peri-implant clinical and radiographic parameters compared to MAs. CLINICAL RELEVANCE: Laser-microtextured abutments may benefit peri-implant clinical and radiographic outcomes.

Evaluation of Vertical Misfit and Torque Loss of Different Abutments for Tri-Channel Type Internal Connection Dental Implants After Mechanical Cycling.

The aim of this study was to evaluate the mechanical behavior of UCLA and Mini-conical abutments for implants with Tri-channel connections regarding torque loss and vertical misfit. Twenty 3-element metal-ceramic fixed partial dentures (FPD) supported by 2 implants were manufactured and divided into 2 groups (n = 10): UCLA (group 1) and Mini-conical Abutments (group 2). The evaluation of torque loss was carried out before and after mechanical cycling, while the vertical fit was evaluated throughout the different stages of manufacturing the prostheses, as well pre- and postcycling (300,000 cycles, 30 N). Statistical analyses of torque loss and vertical misfit were performed using the linear mixed effects model. Both groups showed torque loss after mechanical cycling (P < .05); however, there was no significant percentage differences between them (P = .795). Before cycling, the groups showed a significant difference in terms of vertical misfit values (P < .05); however, this difference was no long observed after cycling (P = .894). Both groups showed torque loss after the cycling test, with no significant difference (P > .05). There was no significant difference in vertical misfit after mechanical cycling; however, in group 1 (UCLA) there was accommodation of the implant-UCLA abutment interface, while group 2 (Mini-conical abutment) did not show changes in the interface with the implant after the test. Both groups behaved similarly regarding the torque loss of the prosthesis retention screws pre- and postmechanical cycling, with greater loss after the test.

Influence of screw channel angulation on reverse torque value and fracture resistance in monolithic zirconia restorations after thermomechanical cycling: an in-vitro study.

BACKGROUND: While the concept of angled screw channels has gained popularity, there remains a scarcity of research concerning the torque loss and fracture strength of monolithic zirconia restorations with various screw channel angulations when exposed to thermomechanical cycling. This in-vitro study aimed to evaluate the reverse torque value and fracture resistance of one-piece screw-retained hybrid monolithic zirconia restorations with angulated screw channels after thermomechanical cycling. METHODS: One-piece monolithic zirconia restorations, with angulated screw channels set at 0°, 15°, and 25° (n = 6 per angulation) were fabricated and bonded to titanium inserts using a dual-cure adhesive resin cement. These assemblies were then screwed to implant fixtures embedded in acrylic resin using an omnigrip screwdriver, and reverse torque values were recorded before and after thermomechanical cycles. Additionally, fracture modes were assessed subsequent to the application of compressive load. One-way ANOVA and Bonferroni post hoc test were used to compare the groups (α = 0.05). RESULTS: The study groups were significantly different regarding the fracture resistance (P = 0.0015), but only insignificantly different in the mean percentage torque loss (P = 0.4400). Specifically, the fracture resistance of the 15° group was insignificantly higher compared to the 0° group (P = 0.9037), but significantly higher compared to the 25° group (P = 0.0051). Furthermore, the fracture resistance of the 0° group was significantly higher than that of the 25° group (P = 0.0114). CONCLUSIONS: One-piece hybrid monolithic zirconia restorations with angulated screw channels can be considered an acceptable choice for angulated implants in esthetic areas, providing satisfactory fracture strength and torque loss.

SURVIVAL RATE OF IMPLANT-SUPPORTED, SINGLE-TOOTH RESTORATIONS BASED ON ZIRCONIA OR METAL ABUTMENT IN PATIENTS WITH TOOTH AGENESIS: A 5-YEARS PROSPECTIVE CLINICAL STUDY.

OBJECTIVES: The primary aim was to investigate survival rate of zirconia versus metal abutments, and the secondary aim was clinical outcomes of all-ceramic versus metal-ceramic crowns on single-tooth implants. METHODS: Patients with tooth-agenesis participated to previously published prospective clinical study with 3-year follow-up were recalled after 5 years. Biological variables included survival and success rate of implants, marginal bone level, modified Plaque and Sulcus Bleeding Index and biological complications. Technical variables included restoration survival rate, marginal adaptation and technical complications. The aesthetic outcome of crowns and peri-implant mucosa in addition to patient-reported outcome were recorded. Descriptive analysis, linear mixed model for quantitative data, or generalized linear mixed model for ordinal categorical data were applied; significance was set to 0.05. RESULTS: Fifty-three patients (mean age: 32.4 years), with 89 implants participated to the 5-years examination. The implants supported 50 zirconia abutments with 50 all-ceramic (AC) crown and 39 metal abutments with 29 metal-ceramic (MC) and 10 AC crowns. The Implant and restoration survival rate was 100% and 96%, respectively. No clinically relevant biological difference between implants supporting metal or zirconia abutments was registered. The technical complications were veneering fracture of AC-crowns (n = 3), crown loosening of MC-crowns (n = 4) and one abutment screw loosening (MC-crown on metal abutment). MC-crowns had significantly better marginal adaptation than AC-crowns (p = .01). AC-crowns had significantly better color and morphology than MC-crowns (p = .01). CONCLUSIONS: Zirconia-based single-tooth restorations are reliable alternative materials to metal-based restorations with favorable biological and aesthetic outcome, and few technical complications.

Cemented versus screw-retained zirconia-based single-implant restorations: 5-year results of a randomized controlled clinical trial.

OBJECTIVES: To compare cemented and screw-retained one-piece zirconia-based restorations in terms of clinical, radiographic, and technical outcomes 5 years after insertion. MATERIALS AND METHODS: Thirty-four patients with single-tooth implants were randomly restored with either a cemented lithium disilicate crown on a one-piece customized zirconia abutment (CEM, 17 patients) or a screw-retained crown based on a directly veneered one-piece customized zirconia abutment (SCREW, 16 patients). All patients were recalled for a baseline examination (7-10 days after crown insertion) and then annually up to 5 years. The following outcomes were assessed: marginal bone level (changes), technical, and clinical (bleeding on probing, plaque control record, probing depth, and keratinized tissue) parameters. The Mann-Whitney U-test was used to assess differences between the two groups. RESULTS: At 5 years, 26 patients (13 in each group) were re-examined. The survival rates on the implant and restorative levels were 100% and 82.4% (equally for both groups), respectively. At 5 years, the median marginal bone level was located at -0.15 mm (IQR: -0.89 mm; 0.27 mm) (CEM) and -0.26 mm (IQR: -0.38 mm; 0.01 mm) (SCREW) below the implant shoulder (intergroup p = .9598). The median changes between baseline and the 5-year follow-up amounted to -0.23 mm (CEM; intragroup p = .0002) and -0.15 mm (SCREW; intragroup p = .1465) (intergroup p = .1690). The overall technical complication rate at 5 years was 15.4% (CEM) and 15.4% (SCREW) (intergroup p = 1.00). Clinical parameters remained stable over time (baseline to 5 years). CONCLUSIONS: At 5 years, screw-retained and cemented restorations rendered largely the same clinical, technical, and radiographic outcomes. Technical complications were frequent in both groups.

Scanning electron microscopic evaluation of microgap size in Morse taper implants with straight and angled abutments.

The aim of this study was to compare the sizes of the implant-abutment microgap in Morse taper implants with a straight abutment (ISA group) and Morse taper implants with an angled abutment (IAA group). A total of 19 Morse taper dental implants (3.8 × 11.0 mm) were used with their respective abutments; 10 abutments were angled at 20°, and 9 were straight. The implant-abutment units were immersed in an epoxy resin to form a base and then cross-sectioned until about 50% of the volume was removed. The specimens were analyzed under a scanning electron microscope, and the size of the microgap was measured at 3 regions on each side of the implant, for a total of 6 measurements per specimen. Mann-Whitney U and Kruskal-Wallis tests were used for statistical analysis. In the ISA group, there were no statistically significant differences in the median sizes of the microgap at the different regions of the interface (P > 0.05; Kruskal-Wallis test). The mean (SD) values of the largest and smallest microgaps in the ISA group were 1.64 (1.38) and 0.83 (0.41) µm, respectively. In the IAA group, there was a statistically significant difference in the median sizes of the microgaps at the upper and middle regions on the left side (P = 0.031; Kruskal-Wallis test). The mean (SD) values of the largest and smallest micrograps were 1.43 (0.062) and 0.61 (0.27) µm, respectively. Comparison of the different regions in the ISA and IAA groups revealed that the only statistically significant difference was at the lower region on the right side (P = 0.027; Mann-Whitney U test). The sizes of the microgaps in the ISA and IAA groups were statistically similar in an overall analysis (P > 0.05; Mann-Whitney U test); however, the IAA group showed greater sealing ability. In addition, in both groups in vitro titanium oxide formation was observed at the interface, characterizing a type of cold-weld joint that provides effective implant-abutment sealing.

Mechanical stability and technical outcomes of monolithic CAD/CAM fabricated abutment-crowns supported by titanium bases: An in vitro study.

OBJECTIVES: To evaluate mechanical stability (survival and complication rates) and bending moments of different all-ceramic monolithic restorations bonded to titanium bases (hybrid abutment-crowns) or to customized titanium abutments compared to porcelain-fused-to-metal crowns (PFM) after thermo-mechanical aging. MATERIAL AND METHODS: Sixty conical connection implants (4.3 mm-diameter) were divided in five groups (n = 12): PFM using gold abutment (GAbut-PFM), lithium disilicate crown bonded to customized titanium abutment (TAbut+LDS), lithium disilicate abutment-crown bonded to titanium base (TiBase+LDS), zirconia abutment-crown bonded to titanium base (TiBase+ZR), polymer-infiltrated ceramic-network (PICN) abutment-crown bonded to titanium base (TiBase+PICN). Simultaneous thermocycling (5°-55°C) and chewing simulation (1,200,000-cycles, 49 N, 1.67 Hz) were applied. Catastrophic and non-catastrophic events were evaluated under light microscope, and survival and complication rates were calculated. Specimens that survived aging were loaded until failure and bending moments were calculated. RESULTS: Survival rates after aging were 100% (TAbut+LDS, TiBase+LDS), 91.7% (GA-PFM), 66.7% (TiBase+ZR) and 58.3% (TiBase+PICN) and differed among the groups (p = .006). Non-catastrophic events as screw loosening (GA-PFM) and loss of retention or micro-/macro-movement (TiBase groups) were observed. Complication rates varied among the groups (p < .001). TiBase+PICN had lower bending moment than all the other groups (p < .001). CONCLUSIONS: Hybrid abutment-crowns made of lithium disilicate can be an alternative to PFM-based restorations, although concerns regarding the bonded interface between the titanium base and abutment-crown can be raised. PICN and zirconia may not be recommended due to its inferior mechanical and bonding outcomes, respectively. Titanium customized abutment with bonded lithium disilicate crown appears to be the most stable combination.

A multicenter cohort study on the association of the one-abutment one-time concept with marginal bone loss around bone level implants.

OBJECTIVES: To investigate the association of the one-abutment one-time concept with marginal bone loss (MBL) around bone-level implants in relation to other factors. MATERIALS AND METHODS: Records from patients treated by four experienced implant surgeons between January 2016 and July 2019 were scrutinized. Subjects treated with two bone-level implant types with varying machined collar (subgroups: 0.5 and 0.8 mm) were considered, receiving a healing abutment (HA cohort) or a permanent abutment at the time of surgery (OT cohort). The primary outcome was MBL registered at 3 months and the longest follow-up. A clustered two-part regression model for semicontinuous data was used. RESULTS: Data pertaining to 160 patients (92 females, mean age 54) and 344 implants (125 in HA cohort, 219 in OT cohort) were available for evaluation. Mean MBL amounted to 0.52 mm (SD 0.68) after a mean follow-up of 20 (SD 9.2) months, with 33.8% of the implants showing complete bone preservation and 5.0% demonstrating >2mm MBL. OT was not related to the presence of MBL using MBL as dependent binary variable (0: no MBL; 1: MBL irrespective of its magnitude). However, OT significantly reduced the magnitude of MBL with 0.300mm when compared to HA (p = .023) in the cases where MBL was detected. Subgroup (p = .212), smoking (p = .789), history of periodontitis (p = .839), type of edentulism (p = .054), implant surgeon (p = .079), patient compliance (p = .617), and follow-up (p = .443) failed to show a significant association with MBL in the regression model. Ninety-eight % of the implants survived. CONCLUSION: Within the limitations of a cohort study, the one-abutment one-time concept was associated with a decrease in MBL at implant sites with bone loss. Therefore, the placement of a permanent abutment at the time of surgery seems relevant to limit marginal bone-level alterations.

Comparison of the Morse Cone Connection with the Internal Hexagon and External Hexagon Connections Based on Microleakage - Review.

The gap formed at the abutment-implant interface brings about a bacterial colonization. In addition, a bacterial reservoir can be established within the implant. The build-up of microorganisms around the implant can cause soft tissue infections and bone loss around the implant, which can lead to implant failure. Our literature review aimed to evaluate the infiltration at the implant-abutment interface, comparing the Morse cone connection with the external hexagon and internal hexagon connections. A literature search using the PubMed database was performed on March 24, 2021. The search terms were combinations of "Morse cone" or "Morse taper" with each of the following terms (individually): "microleakage", "leakage", "infiltration", and "penetration". The inclusion criterion was in vitro studies comparing the Morse cone with the external hexagon and/or internal hexagon, based on infiltration at the implant-abutment interface. The exclusion criterion was the evaluation of microleakage at the implant-abutment interface after applying a sealant over this region. The search was expanded as needed. There was no limit on the year of publication, and only articles written in English were included. In addition, references cited in included articles were also included in this review when they were appropriate. This literature review concluded that, in most cases, the microleakage in the Morse cone connection was lower when compared with the external hexagon and internal hexagon connections.

Implant-prosthetic Rehabilitation with and without Platform Switching: A Retrospective Clinical Cohort Study.

AIM: The study aimed to retrospectively compare peri-implant bone loss, prosthetic complications, and patient-reported outcome measures (PROMs) after implant-prosthetic treatment on abutments with platform switch or platform match. MATERIALS AND METHODS: Records of patients, who received implant-prosthetic treatment on abutments with/without platform switch in a single dental clinic between November 2015 and November 2018, were retrospectively analyzed. Analysis was restricted to the following patient selection criteria: no need for any bone grafting procedures before/during implant placement, and no serious systemic disease. Implants were conventionally loaded with screwed prosthetic restorations after a healing period of 3 months. Crestal bone loss was measured by digital radiography at implant placement and after at least 2 years under functional implant loading conditions. Patient satisfaction was recorded with the visual analogue scale (VAS) at the time of the follow-up examination. RESULTS: Clinical records of 59 patients were available for analysis. Patients of the study cohort received in total 128 implants with different lengths and diameters according to the manufacturer's specifications. Prosthetic restorations were fixed either on abutments with platform switch (BEGO PS-UNI: n = 74; 57.8%) or platform match (BEGO SUB-TEC Universal: n = 54; 42.2%). No implant was lost and no failure of prosthetic restoration was recorded during follow-up, except for prosthetic screw loosening in 32 implants (25.0%). Abutment type and location (maxilla vs mandible) had a significant impact on peri-implant bone loss (OR = 3.4; 2.8). A significant reduced rate of bone loss was observed at implant sites, provided with abutments according to the platform switch concept (35.1 vs 64.8%). No significant correlation was recorded between less bone loss and a higher patient satisfaction, while loosening of the prosthetic screw was significantly associated with lower satisfaction scores. CONCLUSION: BEGO PS-UNI abutments with a platform switch design revealed significant less crestal bone loss after a mean observation period of 20.8 months. CLINICAL SIGNIFICANCE: Abutments with a platform switch design may lead to less peri-implant bone loss. In order to maintain a higher patient satisfaction, clinicians should focus on the quality of the implant-prosthetic connection in screwed restorations.

Cytocompatibility of Titanium, Zirconia and Modified PEEK after Surface Treatment Using UV Light or Non-Thermal Plasma.

A number of modifications have been developed in order to enhance surface cytocompatibility for prosthetic support of dental implants. Among them, ultraviolet (UV) light and non-thermal plasma (NTP) treatment are promising methods. The objective of this study was to compare the effects of UV light and NTP on machined titanium, zirconia and modified polyetheretherketone (PEEK, BioHPP) surfaces in vitro. Machined samples of titanium, zirconia and BioHPP were treated by UV light and NTP of argon or oxygen for 12 min each. Non-treated disks were set as controls. A mouse fibroblast and a human gingival fibroblast cell line were used for in vitro experiments. After 2, 24 and 48 h of incubation, the attachment, viability and cytotoxicity of cells on surfaces were assessed. Results: Titanium, zirconia and BioHPP surfaces treated by UV light and oxygen plasma were more favorable to the early attachment of soft-tissue cells than non-treated surfaces, and the number of cells on those treated surfaces was significantly increased after 2, 24 and 48 h of incubation (p < 0.05). However, the effects of argon plasma treatment on the cytocompatibility of soft tissue cells varied with the type of cells and the treated material. UV light and oxygen plasma treatments may improve the attachment of fibroblast cells on machined titanium, zirconia and PEEK surfaces, that are materials for prosthetic support of dental implants.

Formation of biofilm on various implant abutment materials.

The characteristics of prosthetic implant components, such as the type, material, and surface roughness of abutments, can affect biofilm formation. Since an ideal abutment surface for the reduction of bacterial adhesion has yet to be found, this in vitro study aimed to quantify biofilm formation on laser-treated titanium, zirconia, and titanium surfaces. Sterile titanium, zirconia, and laser-treated titanium discs were placed in sterile 48-well plates. Biofilm formation was induced by adding sterilized, unstimulated human saliva and suspensions of Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Prevotella intermedia (Pi) to the wells. Viable bacteria in the biofilm were quantified with real-time polymerase chain reaction in conjunction with propidium monoazide. The disc material, the type of bacteria, and their interactions had significant effects on the bacterial counts. On all surfaces, the Pg count was significantly higher than both the Pi and Aa counts (P = 0.0001). The highest count of periodontal pathogens was found on laser-treated surfaces. The second highest and the lowest counts were found on zirconia and titanium surfaces, respectively.

Accuracy of Implant Abutment Level Impression with and without Impression Coping.

AIM: Transferring an accurate copy of the patient's soft and hard tissue to the dental laboratory is of essential importance. Various methods of implant impression have different outcomes on dimensions of final cast. This study aimed to compare two methods of implant impression on abutment level with and without impression coping on two parallel implants. MATERIALS AND METHODS: In this experimental study, a resin model with two holes for fixing the implant was made. The first and second implants had a 4 and 11 mm distance to canine respectively. In this study two methods were used for impression: first was direct (without impression coping) and second was indirect (with the impression coping). Ten impressions were prepared for a total of 10 stone casts. For analyzing the abutment analogs positions, each cast was analyzed using a cruicial malformation and malarticulatoin (CMM) in three dimensions (X, Y, and Z). The difference in dimensions of final casts and laboratory models were analyzed using Independent t-test. RESULTS: The results did not show a significant difference between direct and indirect methods in Z and Y axis in absolute transmission (Δr). The dimensional changes in the X-axis in the direct method was 0.647 ± 0.155 which is 0.067 ± 0.146 more than the indirect method. A significant difference (p = 0.044) was observed between the two methods in X-axis. CONCLUSION: Based on the results, this research found the indirect impression on abutment surface to be more accurate than the direct one. In general, two methods were not significantly different, and dentists can use the simple method of the direct impression for making implant prosthetics. CLINICAL SIGNIFICANCE: Reconstruction of implant's accurate position in the process of impression, along with a tension-free insertion, is the first step in having an accurate prosthesis. Abutment level impression with the impression coping is slightly more accurate than the one without impression coping.

Randomized controlled clinical pilot study of all-ceramic single-tooth implant reconstructions: clinical and microbiological outcomes at one year of loading.

OBJECTIVE: To test whether or not pink veneering of the submucosal part of zirconia abutments influences clinical, microbiological and histological outcomes of cemented implant-supported single crowns (ISSC). MATERIALS AND METHODS: A total of 20 patients with one single-tooth implant in the esthetic zone were included. Implants were randomly restored with either pink-veneered zirconia abutments (test group; n = 10) or non-veneered white zirconia abutments (control group; n = 10) and with adhesively cemented all-ceramic crowns. At the 6-month follow-up, soft tissue biopsies were prepared for histological evaluation and microbiological samples were collected around abutments and the respective contra-lateral teeth (in 10 of 20 patients). One year after the initiation of loading, clinical parameters were assessed. Robust linear mixed model and cumulative linked mixed model analyses were performed to investigate the effect of group and time-point on clinical and biological outcomes. RESULTS: Clinical evaluations revealed stable peri-implant soft tissues in terms of probing pocket depth, but a high BOP index (87.5% control; 80.0% test). No statistically significant differences were observed between the test and control group for any outcome measure (P > 0.05). No major biological complications occurred during the observation period. Histological samples revealed a remarkable degree of inflammation in both groups without clear differences in qualitative histological features. Microbiological evaluation demonstrated a slightly higher bacterial count at implants compared to natural teeth at one year of loading without marked differences between groups. CONCLUSION: Limited by a small sample size and a relatively short observation period, pink-veneered zirconia abutments exhibited similar clinical, histological and microbiological outcomes as non-veneered zirconia abutments supporting cemented single crowns.

Influence of Liquid Lubrication on the Screw-Joint Stability of Y-TZP Implant Abutment Systems.

PURPOSE: To evaluate the effectiveness of abutment screws coated with liquid Vaseline on the screw-joint stability of yttria-stabilized tetragonal zirconia (Y-TZP) abutment systems. MATERIALS AND METHODS: Forty Y-TZP prefabricated abutments, 20 Neodent and 20 Bionnovation, were tightened to 20 Ncm on their respective external hexagon implants, and divided into four groups (n = 10) according to the screws: coated with Vaseline or uncoated. The removal torque (RT) value of the abutment screw was measured before and after loading. A cyclic loading (0.5 × 106 cycles; 15 Hz) between 11 and 211 N was applied. Means were compared using a repeated-measures ANOVA (α = 0.05). RESULTS: There was no significant difference between coated and uncoated screws (p = 0.822). Significant differences were found between the abutment systems (p < 0.001). There was a significant effect of cyclic loading (p < 0.001). CONCLUSIONS: Coated and uncoated screws presented similar effectiveness in maintaining preload. CLINICAL RELEVANCE: The liquid lubricant used in this study does not influence the stability of Y-TZP single-implant restorations.

Comparison of pro-inflammatory cytokines and bone metabolism mediators around titanium and zirconia dental implant abutments following a minimum of 6 months of clinical function.

OBJECTIVES: Dental implant abutments are fundamental prosthetic components within dentistry that require optimal biocompatibility. The primary aim of this cross-sectional study was to preliminarily assess differences in the pro-inflammatory cytokine and bone metabolism mediator protein expression in the peri-implant crevicular fluid (PICF) adjacent to transmucosal abutments. MATERIAL AND METHODS: Abutments were fabricated from either titanium or zirconia in patients previously receiving single-tooth implant therapy. All subjects sampled in this study had an identical implant system and implant-abutment connection. Participants (n = 46) had an average time of clinical function for 22 months (6.2-72.8 months, ±SD 17 months) and received a clinical and radiographic examination of the implant site at the time of PICF sampling using a paper strip-based sampling technique. Cytokine, chemokine, and bone metabolism mediator quantities (picograms/30 s) were determined using a commercial 22-multiplexed fluorescent bead-based immunoassay instrument. A total of 19 pro-inflammatory cytokines and seven bone metabolism mediators were evaluated. RESULTS: Multivariable analyses provided no evidence of a group (titanium or zirconia), gender, or age effect with regard to the expression of pro-inflammatory mediators evaluated. Significant (P = 0.022) differences were observed for the bone mediator leptin, with titanium abutments demonstrating significantly elevated levels in comparison with zirconia. Osteopontin demonstrated a significant (P = 0.0044) correlation with age of the subjects. CONCLUSIONS: No significant differences in pro-inflammatory cytokine or bone metabolism mediator profiles were observed biochemically, with the exception of leptin, for the abutment biomaterials of titanium or zirconia The molecular PICF findings support the observed clinical biocompatibility of both titanium and zirconia abutments.

Surface properties correlated with the human gingival fibroblasts attachment on various materials for implant abutments: a multiple regression analysis.

OBJECTIVES: To reveal the suitable surface condition of an implant abutment for fibroblast attachment, the correlation between the surface characteristics of various materials and the human gingival fibroblast (HGF-1) attachment to the surfaces were analyzed. METHODS: Six kinds of surfaces comprised of machined titanium alloy (SM), machined Co-Cr-Mo alloy (CCM), titanium nitride coated titanium alloy (TiN), anodized titanium alloy (AO), composite resin coating on titanium alloy (R) and zirconia (Zr) were used. The measured surface parameters were Sa, Sq, Sz, Sdr, Sdq, Sal, Str and water contact angle (WCA). The HGF-1 cell attachment was investigated and the correlations were analyzed using a multiple regression analysis. RESULTS: The HGF-1 cell attachment was greater in the SM, TiN and Zr groups than the other groups and smallest in the CCM group (p = 0.0096). From the multiple regression analysis, the HGF-1 cell attachment was significantly correlated with Sdr, Sdq and WCA. When the R group was excluded, only WCA showed significant correlation with the fibroblast attachment. CONCLUSIONS: Within the limitations of this study, the cell attachment of human gingival fibroblasts was correlated with WCA, developed interfacial area ratio and surface slope. When the surfaces with Sa values of ∼ 0.2 µm or less were concerned, only WCA showed a correlation in a third order manner.