Subtetanic neuromuscular electrical stimulation can maintain Wingate test performance but augment blood lactate accumulation.

PURPOSE: Concentration- and time-dependent effect of lactate on physiological adaptation (i.e., glycolytic adaptation and mitochondrial biogenesis) have been reported. Subtetanic neuromuscular electrical stimulation (NMES) with voluntary exercise (VOLES) can increase blood lactate accumulation. However, whether this is also true that VOLES can enhance the blood lactate accumulation during sprint exercise is unknown. Thus, we investigated whether VOLES before the Wingate test can enhance blood lactate accumulation without compromising Wingate exercise performance. METHODS: Fifteen healthy young males (mean [SD], age: 23 [4] years, body mass index: 22.0 [2.1] kg/m2) volunteered. After resting measurement, participants performed a 3-min intervention: VOLES (NMES with free-weight cycling) or voluntary cycling alone, which matched exercise intensity with VOLES (VOL, 43.6 [8.0] watt). Then, they performed the Wingate test with 30 min free-weight cycling recovery. The blood lactate concentration ([La]b) was assessed at the end of resting and intervention, and recovery at 1, 3, 5, 10, 20, and 30 min. RESULTS: [La]b during intervention was higher with VOLES than VOL (P = 0.011). The increase in [La]b after the Wingate test was maintained for longer with VOLES than VOL at 10- and 20-min recovery (P = 0.014 and 0.023, respectively). Based on the Wingate test, peak power, mean power, and the rate of decline were not significantly different between VOLES and VOL (P = 0.184, 0.201, and 0.483, respectively). CONCLUSION: The combination of subtetanic NMES with voluntary exercise before the Wingate test has the potential to enhance blood lactate accumulation. Importantly, this combined approach does not compromise Wingate exercise performance compared to voluntary exercise alone.

Effects of NMES pulse width and intensity on muscle mechanical output and oxygen extraction in able-bodied and paraplegic individuals.

PURPOSE: Neuromuscular Electrical Stimulation (NMES) is commonly used in neuromuscular rehabilitation protocols, and its parameters selection substantially affects the characteristics of muscle activation. Here, we investigated the effects of short pulse width (200 µs) and higher intensity (short-high) NMES or long pulse width (1000 µs) and lower intensity (long-low) NMES on muscle mechanical output and fractional oxygen extraction. Muscle contractions were elicited with 100 Hz stimulation frequency, and the initial torque output was matched by adjusting stimulation intensity. METHODS: Fourteen able-bodied and six spinal cord-injured (SCI) individuals participated in the study. The NMES protocol (75 isometric contractions, 1-s on-3-s off) targeting the knee extensors was performed with long-low or short-high NMES applied over the midline between anterior superior iliac spine and patella protrusion in two different days. Muscle work was estimated by torque-time integral, contractile properties by rate of torque development and half-relaxation time, and vastus lateralis fractional oxygen extraction was assessed by Near-Infrared Spectroscopy (NIRS). RESULTS: Torque-time integral elicited by the two NMES paradigms was similar throughout the stimulation protocol, with differences ranging between 1.4% (p = 0.877; able-bodied, mid-part of the protocol) and 9.9% (p = 0.147; SCI, mid-part of the protocol). Contractile properties were also comparable in the two NMES paradigms. However, long-low NMES resulted in higher fractional oxygen extraction in able-bodied (+ 36%; p = 0.006). CONCLUSION: Long-low and short-high NMES recruited quadriceps femoris motor units that demonstrated similar contractile and fatigability properties. However, long-low NMES conceivably resulted in the preferential recruitment of vastus lateralis muscle fibers as detected by NIRS.

The plateau in the NIRS-derived [HHb] signal near the end of a ramp incremental test does not indicate the upper limit of O2 extraction in the vastus lateralis.

This study aimed to examine, at the level of the active muscles, whether the plateau in oxygen (O2) extraction normally observed near the end of a ramp incremental (RI) exercise test to exhaustion is caused by the achievement of an upper limit in O2 extraction. Eleven healthy men (27.3 ± 3.0 yr, 81.6 ± 8.1 kg, 183.9 ± 6.3 cm) performed a RI cycling test to exhaustion. O2 extraction of the vastus lateralis (VL) was measured continuously throughout the test using the near-infrared spectroscopy (NIRS)-derived deoxygenated hemoglobin [HHb] signal. A leg blood flow occlusion was performed at rest (LBFOCC1) and immediately after the RI test (LBFOCC2). The [HHb] values during the resting occlusion (108.1 ± 21.7%; LBFOCC1) and the peak values during exercise (100 ± 0%; [HHb]plateau) were significantly greater than those observed at baseline (0.84 ± 10.6% at baseline 1 and 0 ± 0% at baseline 2) (P < 0.05). No significant difference was found between LBFOCC1 and [HHb]plateau (P > 0.05) or between the baseline measurements (P > 0.05). [HHb] values at LBFOCC2 (130.5 ± 19.7%) were significantly greater than all other time points (P < 0.05). These results support the existence of an O2 extraction reserve in the VL muscle at the end of a RI cycling test and suggest that the observed plateau in the [HHb] signal toward the end of a RI test is not representative of an upper limit in O2 extraction.

Muscle Oxidative Capacity in Vivo Is Associated With Physiological Parameters in Trained Rowers.

Purpose: The muscle oxygen uptake (mV˙O2) kinetics following exercise, measured by near-infrared spectroscopy, has been used as a functional evaluation of muscle oxidative metabolism. This study aimed to determine the mV˙O2 off-kinetics and verify the relationship of the recovery rate of mV˙O2 (k) with time-trial performance and different aerobic parameters in trained rowers. Methods: Eleven male rowers (age: 20 ± 3 years; V˙O2max: 4.28 ± 0.35 L·min-1) used a rowing ergometer to perform (I) an incremental test to determine the maximal oxygen uptake (V˙O2max) and peak power output (Ppeak); (II) several visits to determine maximal lactate steady state (MLSS); and (III) a 2000-m rowing ergometer performance test. Also, one test to determine mV˙O2 off-kinetics of the vastus lateralis muscle using a repeated arterial occlusions protocol. Results: The mV˙O2 generated a good monoexponential fit (R2 = 0.960 ± 0.030; SEE = 0.041 ± 0.018%.s-1). The k of mV˙O2 (2.06 ± 0.58 min-1) was associated with relative V˙O2max (r = 0.79), power output at MLSS (r = 0.76), and Ppeak (r = 0.83); however, it was not related with 2000-m rowing performance (r = -0.38 to 0.52; p > .152). Conclusion: These findings suggest that although not associated with rowing performance, the mV˙O2 off-kinetics determined after a submaximal isometric knee extension may be a practical and less-exhaustive approach than invasive responses and incremental tests to assess the muscle oxidative metabolism during a training program.

1) The present study showed that the NIRS-derived mV˙O2 off-kinetics from the vastus lateralis can be used as a functional tool of skeletal muscle oxidative metabolism in rowers.2) The results demonstrated that the recovery rate of mV˙O2 was well correlated with important aerobic parameters such as V˙O2max, maximal lactate steady state, and peak power output during an incremental test.

Combining Chronic Ischemic Preconditioning and Inspiratory Muscle Warm-Up to Enhance On-Ice Time-Trial Performance in Elite Speed Skaters.

Elite athletes in varied sports typically combine ergogenic strategies in the hope of enhancing physiological responses and competitive performance, but the scientific evidence for such practices is very scarce. The peculiar characteristics of speed skating contribute to impede blood flow and exacerbate deoxygenation in the lower limbs (especially the right leg). We investigated whether combining preconditioning strategies could modify muscular oxygenation and improve performance in that sport. Using a randomized, single-blind, placebo-controlled, crossover design, seven male elite long-track speed skaters performed on-ice 600-m time trials, preceded by either a combination of preconditioning strategies (COMBO) or a placebo condition (SHAM). COMBO involved performing remote ischemic preconditioning (RIPC) of the upper limbs (3 × 5-min compression at 180 mmHg and 5-min reperfusion) over 3 days (including an acute treatment before trials), with the addition of an inspiratory muscle warm-up [IMW: 2 × 30 inspirations at 40% maximal inspiratory pressure (MIP)] on the day of testing. SHAM followed the same protocol with lower intensities (10 mmHg for RIPC and 15% MIP). Changes in tissue saturation index (TSI), oxyhemoglobin-oxymyoglobin ([O2HbMb]), deoxyhemoglobin-deoxymyoglobin ([HHbMb]), and total hemoglobin-myoglobin ([THbMb]) in the right vastus lateralis muscle were monitored by near-infrared spectroscopy (NIRS). Differences between COMBO and SHAM were analyzed using Cohen's effect size (ES) and magnitude-based inferences. Compared with SHAM, COMBO had no worthwhile effect on performance time while mean Δ[HHbMb] (2.7%, ES 0.48; -0.07, 1.03) and peak Δ[HHbMb] (1.8%, ES 0.23; -0.10, 0.57) were respectively likely and possibly higher in the last section of the race. These results indicate that combining ischemic preconditioning and IMW has no practical ergogenic impact on 600-m speed-skating performance in elite skaters. The low-sitting position in this sport might render difficult enhancing these physiological responses.