No evidence that modification of soil microbiota by woody invader facilitates subsequent invasion by herbaceous species.

Many terrestrial ecosystems are co-invaded by multiple exotic species. The "invasional meltdown" hypothesis predicts that an initial invasive species will facilitate secondary invasions. In the plant kingdom, the potential underlying mechanisms of this hypothesis may be that modification of the soil properties by the initial invaders benefits for the subsequent exotic species invasion. In this study, we analyzed the composition of soil microbial communities and soil chemical properties from sites invaded by woody Rhus typhina, as well as uninvaded sites, to assess the impact of R. typhina invasion. Furthermore, we conducted a greenhouse experiment with multiple native-invasive pairs of herbaceous species to test whether R. typhina invasion facilitates subsequent exotic herb invasion. Our results showed that R. typhina invasion significantly altered the composition of soil fungal communities, especially pathogenic, endophytic, and arbuscular mycorrhizal fungi. However, this change in microbial composition led to neither direction nor magnitude changes in negative plant-soil feedback effects on both native and invasive species. This indicates that initial R. typhina invasion does not facilitate subsequent herb invasion, which does not support the "invasional meltdown" hypothesis. Additionally, R. typhina invasion significantly decreased soil total nitrogen and organic carbon contents, which may explain the significantly lower biomass of herbaceous roots grown in invaded soils compared with uninvaded soils. Alternately, although invasive herb growth was significantly more inhibited by soil microbiota compared with native herb growth, such inhibition cannot completely eliminate the risk of exotic herb invasion because of their innate growth advantages. Therefore, microbial biocontrol agents for plant invasion management should be combined with another approach to suppress the innate growth advantages of exotic species.

Mapping the meltdown behavior of frozen dairy desserts.

The meltdown test is an efficient tool widely and commonly used to characterize structural changes in frozen desserts resulting from different ingredients and processing conditions. The meltdown is commonly determined by a gravimetric test, and it is used to obtain the onset (Mon), rate (Mrate), and maximum (MMax) meltdown. However, these parameters are calculated ambiguously due to inconsistencies in the methodology. This work aims to model the meltdown curves (weight vs. time) of different commercial frozen dessert samples (36 commercial samples). Samples of commercial frozen desserts (40-60 g) was placed on a 304 stainless wire cloth (1.50 mm opening size and 52% open area) suspended ∼15 cm above an analytical balance, and the dripped portion of the melted sample was continuously recorded throughout the duration of the test. The meltdown test was conducted at room temperature. Each meltdown test generated between 3,000 to 4,000 data points and was modeled using 4 equations: the logistic model, the Gompertz model, the Richard model, and the Hill model. All the meltdown curves were sigmoidal in shape, regardless of the type of frozen dessert. The experimental meltdown curves were adequately represented by the logistic model, judging by several criteria (R2 = 0.999, RAdj2 = 0.999, Akaike information criterion = 6,582, and F-value = 1.88 × 106). Thus, the logistic model was shown to be an effective tool for predicting the meltdown curves of frozen desserts, and it can be used to unambiguously define Mon, Mrate, and MMax. Moreover, a dimensionless response (meltdown behavior, MBe) that combines Mon, Mrate, and MMax was developed and used for mapping the meltdown of different commercial frozen desserts.

Imaging the 3D microstructural changes of ice cream during meltdown.

Ice cream is a multiphase frozen dessert that often melts during distribution and upon consumption. The meltdown phenomenon is one of the concerns in the quality preservation of ice cream for consumer convenience in the frozen food industry. In this context, X-ray tomography was used to visualise and quantify 3D ice crystal and air bubble evolution during the meltdown of ice cream. Two ice cream products, namely I and II, with varying air volume fractions, were evaluated for this study. The results indicated a small mean diameter of 66.43 ± 2.07 µm at 0 min and decreased to 45.74 ± 3.92 µm during 10 min of the meltdown of ice cream II. A large mean diameter of ice crystals of 75.02 ± 3.14 µm was found in ice cream I, at 0 min that decreased significantly (p < 0.05) to 54.30 ± 2.63 µm during 10 min of the meltdown. The air bubbles were also observed to decrease in mean diameter. The 3D datasets on the ice crystals and air bubbles described in this work provide more insight into the 3D microstructural evolution during the meltdown and are useful in controlling the sensory quality attributes of ice cream desserts.

Mitonuclear Compensatory Coevolution.

In bilaterian animals, the mitochondrial genome is small, haploid, does not typically recombine, and is subject to accumulation of deleterious alleles via Muller's ratchet. These basic features of the genomic architecture present a paradox: mutational erosion of these genomes should lead to decline in mitochondrial function over time, yet no such decline is observed. Compensatory coevolution, whereby the nuclear genome evolves to compensate for the deleterious alleles in the mitochondrial genome, presents a potential solution to the paradox of Muller's ratchet without loss of function. Here, I review different proposed forms of mitonuclear compensatory coevolution. Empirical evidence from diverse eukaryotic taxa supports the mitonuclear compensatory coevolution hypothesis, but the ubiquity and importance of such compensatory coevolution remains a topic of debate.

Partial Clonality Expands the Opportunity for Spatial Adaptation.

AbstractReproductive mode may strongly impact adaptation in spatially varying populations linked by dispersal, especially when sexual and clonal offspring differ in dispersal. We determined how spatial structure affects adaptation in populations with mixed clonal and sexual reproduction. In a source-sink quantitative genetic deterministic model (with stabilizing selection around different optima), greater clonal reproduction or parent-offspring association (a measure of the part of the parent's phenotype other than the additive genetic component inherited by clonal offspring) increased the selective difference (difference between phenotypic optima) allowing sink populations to adapt. Given dispersal differences between clonally and sexually produced juveniles, adaptation increased with an increasing fraction of clonal dispersers. When considering migrational meltdown, partially clonal reproduction reduced cases where dispersal caused habitat loss. Stochastic individual-based simulations support these results, although the effect of differential dispersal was reversed, with decreased clonal dispersal allowing greater adaptation. These results parallel earlier findings that for an instantaneous shift in phenotypic optimum, increasing clonality allowed population persistence for a greater shift; here, selective change is spatial rather than temporal. These results may help explain the success of many partially clonal organisms in invading new habitats, complementing traditional explanations based on avoiding Allee effects.

Mutational meltdown in asexual populations doomed to extinction.

Asexual populations are expected to accumulate deleterious mutations through a process known as Muller's ratchet. Lynch and colleagues proposed that the ratchet eventually results in a vicious cycle of mutation accumulation and population decline that drives populations to extinction. They called this phenomenon mutational meltdown. Here, we analyze mutational meltdown using a multi-type branching process model where, in the presence of mutation, populations are doomed to extinction. We analyse the change in size and composition of the population and the time of extinction under this model.

Partition of milk phospholipids during ice cream manufacturing.

The distribution of phospholipids (PL) within the fat and serum phase of ice cream manufacturing was evaluated through partition coefficients (KPL) after mixing, pasteurization, freezing, and hardening. Ice creams containing about 40.41 ± 3.45 (± standard deviation; control formulation) and 112.29 ± 9.06 (enriched PL formulation) mg of PL per g of fat were formulated with nonfat dry milk and ß-serum, respectively. Overall, the KPL were lower than 1, indicating that the PL were predominantly found in the fat phase, and only a small amount was left in the serum and sediment. Confocal micrographs visually confirmed this generalization. The addition of PL significantly increased the viscosity of the mixes between 4- and 9-fold, depending on the shear rate. Additionally, mixes containing high PL exhibited higher yield stress than those formulated with low PL (0.15 ± 0.09 and 0.016 ± 0.08 Pa, respectively). Ice creams with high PL delayed the onset of meltdown and exhibited a slower rate of a meltdown than low-PL ice creams (18.53 ± 0.57 and 14.83 ± 0.85 min, and 1.01 ± 0.05 and 0.71 ± 0.04% min-1, respectively). This study provides useful guidelines for manufacturing ice cream enriched in milk PL. Additionally, the use of ß-serum, a byproduct stream, as a source of PL is illustrated. The development will require studying the sensorial description of the product as well as consumer acceptance.

Resource limitation determines realized thermal performance of consumers in trophodynamic models.

Recent work has demonstrated that changes in resource availability can alter a consumer's thermal performance curve (TPC). When resources decline, the optimal temperature and breadth of thermal performance also decline, leading to a greater risk of warming than predicted by static TPCs. We investigate the effect of temperature on coupled consumer-resource dynamics, focusing on the potential for changes in the consumer TPC to alter extinction risk. Coupling consumer and resource dynamics generally reduces the potential for resource decline to exacerbate the effects of warming via changes to the TPC due to a reduction in top-down control when consumers near the limits of their thermal performance curve. However, if resources are more sensitive to warming, consumer TPCs can be reshaped by declining resources, leading to increased extinction risk. Our work elucidates the role of top-down and bottom-up regulation in determining the extent to which changes in resource density alter consumer TPCs.

Positive interactions of native species melt invasional meltdown over long-term plant succession.

Positive interactions have been hypothesised to influence plant community dynamics and species invasions. However, their prevalence and importance relative to negative interactions remain unclear to understand community change and invasibility. We examined pairwise biotic interactions using over 50 years of successional data to assess the prevalence of positive interactions and their effects on each focal species (either native or exotic). We found that positive interactions were widespread and the relative frequency of positive and negative interactions varied with establishment stage and between native and exotic species. Specifically, positive interactions were more frequent during early establishment and less frequent at later stages. Positive interactions involving native species were more frequent and stronger than those between exotic species, reducing the importance of invasional meltdown on succession. Our study highlights the role of positive native interactions in shielding communities from biological invasion and enhancing the potential for long-term resilience.

Opposite effects of nutrient enrichment and an invasive snail on the growth of invasive and native macrophytes.

Many ecosystems are now co-invaded by alien plant and herbivore species. The evolutionary naivety of native plants to alien herbivores can make the plants more susceptible to the detrimental effects of herbivory than co-occurring invasive plants, in accordance with the apparent competition hypothesis. Moreover, the invasional meltdown hypothesis predicts that in multiply invaded ecosystems, invasive species can facilitate each other's impacts on native communities. Although there is growing empirical support for these hypotheses, facilitative interactions between invasive plants and herbivores remain underexplored in aquatic ecosystems. Many freshwater ecosystems are co-invaded by aquatic macrophytes and mollusks and simultaneously experience nutrient enrichment. However, the interactive effects of these ecological processes on native macrophyte communities remain an underexplored area. To test these effects, we performed a freshwater mesocosm experiment in which we grew a synthetic native community of three macrophyte species under two levels of invasion by an alien macrophyte Myriophyllum aquaticum (invasion vs. no invasion) and fully crossed with two levels of nutrient enrichment (enrichment vs. no enrichment) and herbivory by an invasive snail Pomacea canaliculata (herbivory vs. no herbivory). In line with the invasional meltdown and apparent competition hypotheses, we found that the proportional aboveground biomass yield of the invasive macrophyte, relative to that of the native macrophyte community, was significantly greater in the presence of the invasive herbivore. Evidence of a reciprocal facilitative effect of the invasive macrophyte on the invasive herbivore is provided by results showing that the herbivore produced greater egg biomass in the presence versus in the absence of M. aquaticum. However, nutrient enrichment reduced the mean proportional aboveground biomass yield of the invasive macrophyte. Our results suggested that herbivory by invader P. canaliculata may enhance the invasiveness of M. aquaticum. However, nutrient enrichment of habitats that already harbor M. aquaticum may slow down the invasive spread of the macrophyte. Broadly, our study underscores the significance of considering several factors and their interactions when assessing the impact of invasive species, especially considering that many habitats experience co-invasion by plants and herbivores and simultaneously undergo various other disturbances, including nutrient enrichment.

Vulnerability to Fishing and Life History Traits Correlate with the Load of Deleterious Mutations in Teleosts.

Understanding why some species accumulate more deleterious substitutions than others is an important question relevant in evolutionary biology and conservation sciences. Previous studies conducted in terrestrial taxa suggest that life history traits correlate with the efficiency of purifying selection and accumulation of deleterious mutations. Using a large genome data set of 76 species of teleostean fishes, we show that species with life history traits associated with vulnerability to fishing have an increased rate of deleterious mutation accumulation (measured via dN/dS, i.e., nonsynonymous over synonymous substitution rate). Our results, focusing on a large clade of aquatic species, generalize previous patterns found so far in few clades of terrestrial vertebrates. These results also show that vulnerable species to fishing inherently accumulate more deleterious substitutions than nonthreatened ones, which illustrates the potential links among population genetics, ecology, and fishing policies to prevent species extinction.

Native and alien species suffer from late arrival, while negative effects of multiple alien species on natives vary.

Ongoing globalisation and climate change are causing plant species to invade new habitats and thereby alter biodiversity and ecosystem functioning. Since numbers of plant invasions continue to increase globally, it is crucial to investigate the effects of multiple co-occurring alien species on native communities. Furthermore, priority effects due to the earlier emergence of certain species affecting fitness of later arriving species can shape community structure and affect native species performance. We investigate in a common garden pot experiment the interactions among five alien-native species pairs. First we focus on the effect of growing with either one or two alien neighbour species on a native plant, second we alter the arrival time of the alien or native neighbour by 3 weeks. Generally, native species performance decreased when surrounded by two alien species compared to only one, although the magnitude of this effect varied depending on species, with one species even performing better with alien neighbours than in monoculture. Species performance greatly decreased when arriving second in the pot, for both native and alien species. In contrast, alien species tended to benefit more from arriving early. Given that we studied annual ruderal species, their potentially lower competitive ability might explain why we detected negative effects of late arrival. We highlight the need to further elucidate underlying mechanisms of small-scale invasion dynamics to achieve generalisations concerning the response of multiple alien and native plants given their species-specific differences in response to neighbour species and arrival time.

Disruption of cross-feeding interactions by invading taxa can cause invasional meltdown in microbial communities.

The strength of biotic interactions within an ecological community affects the susceptibility of the community to invasion by introduced taxa. In microbial communities, cross-feeding is a widespread type of biotic interaction that has the potential to affect community assembly and stability. Yet, there is little understanding of how the presence of cross-feeding within a community affects invasion risk. Here, I develop a metabolite-explicit model where native microbial taxa interact through both cross-feeding and competition for metabolites. I use this model to study how the strength of biotic interactions, especially cross-feeding, influence whether an introduced taxon can join the community. I found that stronger cross-feeding and competition led to much lower invasion risk, as both types of biotic interactions lead to greater metabolite scarcity for the invader. I also evaluated the impact of a successful invader on community composition and structure. The effect of invaders on the native community was greatest at intermediate levels of cross-feeding; at this 'critical' level of cross-feeding, successful invaders generally cause decreased diversity, decreased productivity, greater metabolite availability, and decreased quantities of metabolites exchanged among taxa. Furthermore, these changes resulting from a successful primary invader made communities further susceptible to future invaders. The increase in invasion risk was greatest when the network of metabolite exchange between taxa was minimally redundant. Thus, this model demonstrates a case of invasional meltdown that is mediated by initial invaders disrupting the metabolite exchange networks of the native community.

Phylogenetically diverse native systems are more resistant to invasive plant species on Robben Island, South Africa 1.

Alien invasive species are problematic both economically and ecologically, particularly on islands. As such, understanding how they interact with their environment is necessary to inform invasive species management. Here, we ask the following questions: What are the main functional traits that correlate with invasion success of alien plants on Robben Island? How does phylogenetic structure shape biotic interactions on the island? Using multiple approaches to explore these questions, we found that alien invasive species flower later during the year and for longer period, although flowering phenology was sensitive to alternative starting date. Additionally, we observed that alien invasive species are mostly abiotically pollinated and are generally hermaphroditic whilst their native counterparts rely on biotic pollinators, flower earlier, and are generally dioecious, suggesting that alien invasive and native species use different ecological niches. Furthermore, we found a facilitative interaction between an alien invasive legume and other invasive plants as predicted by the invasional meltdown hypothesis, but this does not influence the phylogenetic structure of plant communities. Finally, phylogenetically diverse set of native species are less receptive to alien invasive species. Collectively, our findings reveal how biotic interactions and phylogenetic relatedness structure alien invasive - native co-existence.

Stochastic Modeling and Simulation of Viral Evolution.

RNA viruses comprise vast populations of closely related, but highly genetically diverse, entities known as quasispecies. Understanding the mechanisms by which this extreme diversity is generated and maintained is fundamental when approaching viral persistence and pathobiology in infected hosts. In this paper, we access quasispecies theory through a mathematical model based on the theory of multitype branching processes, to better understand the roles of mechanisms resulting in viral diversity, persistence and extinction. We accomplish this understanding by a combination of computational simulations and the theoretical analysis of the model. In order to perform the simulations, we have implemented the mathematical model into a computational platform capable of running simulations and presenting the results in a graphical format in real time. Among other things, we show that the establishment of virus populations may display four distinct regimes from its introduction into new hosts until achieving equilibrium or undergoing extinction. Also, we were able to simulate different fitness distributions representing distinct environments within a host which could either be favorable or hostile to the viral success. We addressed the most used mechanisms for explaining the extinction of RNA virus populations called lethal mutagenesis and mutational meltdown. We were able to demonstrate a correspondence between these two mechanisms implying the existence of a unifying principle leading to the extinction of RNA viruses.

The fundamental theorem of natural selection with mutations.

The mutation-selection process is the most fundamental mechanism of evolution. In 1935, R. A. Fisher proved his fundamental theorem of natural selection, providing a model in which the rate of change of mean fitness is equal to the genetic variance of a species. Fisher did not include mutations in his model, but believed that mutations would provide a continual supply of variance resulting in perpetual increase in mean fitness, thus providing a foundation for neo-Darwinian theory. In this paper we re-examine Fisher's Theorem, showing that because it disregards mutations, and because it is invalid beyond one instant in time, it has limited biological relevance. We build a differential equations model from Fisher's first principles with mutations added, and prove a revised theorem showing the rate of change in mean fitness is equal to genetic variance plus a mutational effects term. We refer to our revised theorem as the fundamental theorem of natural selection with mutations. Our expanded theorem, and our associated analyses (analytic computation, numerical simulation, and visualization), provide a clearer understanding of the mutation-selection process, and allow application of biologically realistic parameters such as mutational effects. The expanded theorem has biological implications significantly different from what Fisher had envisioned.

Strong indirect herbicide effects on mycorrhizal associations through plant community shifts and secondary invasions.

Million of acres of U.S. wildlands are sprayed with herbicides to control invasive species, but relatively little is known about non-target effects of herbicide use. We combined greenhouse, field, and laboratory experiments involving the invasive forb spotted knapweed (Centaurea stoebe) and native bunchgrasses to assess direct and indirect effects of the forb-specific herbicide picloram on arbuscular mycorrhizal fungi (AMF), which are beneficial soil fungi that colonize most plants. Picloram had no effect on bunchgrass viability and their associated AMF in the greenhouse, but killed spotted knapweed and reduced AMF colonization of a subsequent host grown. Results were similar in the field where AMF abundance in bunchgrass-dominated plots was unaffected by herbicides one year after spraying based on 16:1ω5 phospholipid fatty acid (PLFA) and neutral lipid fatty acid (NLFA) concentrations. In spotted-knapweed-dominated plots, however, picloram application shifted dominance from spotted knapweed, a good AMF host, to bulbous bluegrass (Poa bulbosa), a poor AMF host. This coincided with a 63% reduction in soil 16:1ω5 NLFA concentrations but no reduction of 16:1ω5 PLFA. Because 16:1ω5 NLFA quantifies AMF storage lipids and 16:1ω5 PLFA occurs in AMF membrane lipids, we speculate that the herbicide-mediated reduction in host quality reduced fungal carbon storage, but not necessarily fungal abundance after one year in the field. Overall, in greenhouse and field experiments, AMF were only affected when picloram altered host quantity and quality. This apparent lack of direct effect was supported by our in-vitro trial where picloram applied to AMF mycelia did not reduce fungal biomass and viability. We show that the herbicide picloram can have profound, indirect effects on AMF within one year. Depending on herbicide-mediated shifts in host quality, rapid interventions may be necessary post herbicide applications to prevent loss of AMF abundance. Future research should assess consequences of these potential shifts for the restoration of native plants that differ in mycorrhizal dependency.

Habitat augmentation drives secondary invasion: an experimental approach to determine the mechanism of invasion success.

The entry of secondary invaders into, or their expansion within, native communities is contingent on the changes wrought by other (primary) invaders. When primary invaders have altered more than one property of the recipient community, standard descriptive and modeling approaches only provide a best guess of the mechanism permitting the secondary invasion. In rainforest on Christmas Island, we conducted a manipulative field experiment to determine the mechanism of invasion success for a community of land snails dominated by non-native species. The invasion of rainforest by the yellow crazy ant (Anoplolepis gracilipes) has facilitated these land snails, either by creating enemy-free space and/or increased habitat and resources (in the form of leaf litter) through the removal of the native omnivorous-detritivorous red land crab (Gecarcoidea natalis). We manipulated predator densities (high and low) and leaf litter (high and low) in replicated blocks of four treatment combinations at two sites. Over the course of one wet season (five months), we found that plots with high leaf litter biomass contained significantly more snails than those with low biomass, regardless of whether those plots had high or low predation pressure, at both the site where land crabs have always been abundant, and at the site where they have been absent for many years prior to the experiment. Each site was dominated by small snail species (<2 mm length), and through handling size and predation experiments we demonstrated that red crabs tend not to handle and eat snails of that size. These results suggest that secondary invasion by this community of non-native land snails is facilitated most strongly by habitat and resource augmentation, an indirect consequence of red land crab removal, and that the creation of enemy-free space is not important. By using a full-factorial experimental approach, we have confidently determined-rather than inferred-the mechanism by which primary invaders indirectly facilitate a community of secondary invaders.

Cross-Sectional Data Within 1 Year of the Fukushima Meltdown: Effect-Size of Predictors for Depression.

This cross-sectional study investigates effect sizes of depression predictors in a community close to the Fukushima, Japan nuclear reactor damaged by the 11 March, 2011 earthquake and tsunami. Subjects volunteered for assessment between December, 2011 and March, 2012. Of 466 individuals (351 female, mean age 60.4 year, SD = 14.0), 23 % of the female participants and 17 % of the male participants could be diagnosed with depression. The strongest predictors were house damage, age, income reduction, home water incursion, and casualty acquaintance. Education level, location during disaster, and workplace damage proved non-significant. The high number of retired/unemployed in the sample may have influenced outcome. Results suggest sampling influences the applicability of Conservation of Resources model to a disaster event.

Mutualism between co-introduced species facilitates invasion and alters plant community structure.

Generalized mutualisms are often predicted to be resilient to changes in partner identity. Variation in mutualism-related traits between native and invasive species however, can exacerbate the spread of invasive species ('invasional meltdown') if invasive partners strongly interact. Here we show how invasion by a seed-dispersing ant (Myrmica rubra) promotes recruitment of a co-introduced invasive over native ant-dispersed (myrmecochorous) plants. We created experimental communities of invasive (M. rubra) or native ants (Aphaenogaster rudis) and invasive and native plants and measured seed dispersal and plant recruitment. In our mesocosms, and in laboratory and field trials, M. rubra acted as a superior seed disperser relative to the native ant. By contrast, previous studies have found that invasive ants are often poor seed dispersers compared with native ants. Despite belonging to the same behavioural guild, seed-dispersing ants were not functionally redundant. Instead, native and invasive ants had strongly divergent effects on plant communities: the invasive plant dominated in the presence of the invasive ant and the native plants dominated in the presence of the native ant. Community changes were not due to preferences for coevolved partners: variation in functional traits of linked partners drove differences. Here, we show that strongly interacting introduced mutualists can be major drivers of ecological change.