Simultaneous Biogas Upgrading and Valuable Chemical Production Using Homoacetogens in a Membrane Biofilm Reactor.

Biogas produced from anaerobic digestion usually contains impurities, particularly with a high content of CO2 (15-60%), thus decreasing its caloric value and limiting its application as an energy source. H2-driven biogas upgrading using homoacetogens is a promising approach for upgrading biogas to biomethane and converting CO2 to acetate simultaneously. Herein, we developed a novel membrane biofilm reactor (MBfR) with H2 and biogas separately supplied via bubbleless hollow fiber membranes. The gas-permeable hollow fibers of the MBfR enabled high H2 and CO2 utilization efficiencies (∼98% and ∼97%, respectively) and achieved concurrent biomethane (∼94%) and acetate (∼450 mg/L/d) production. High-throughput 16S rRNA gene amplicon sequencing suggested that enriched microbial communities were dominated by Acetobacterium (38-48% relative abundance). In addition, reverse transcription quantitative PCR of the functional marker gene formyltetrahydrofolate synthetase showed that its expression level increased with increasing H2 and CO2 utilization efficiencies. These results indicate that Acetobacterium plays a key role in CO2 to acetate conversion. These findings are expected to facilitate energy-positive wastewater treatment and contribute to the development of a new solution to biogas upgrading.

Co-Removal of Perfluorooctanoic Acid and Nitrate from Water by Coupling Pd Catalysis with Enzymatic Biotransformation.

PFAS (poly- and per-fluorinated alkyl substances) represent a large family of recalcitrant organic compounds that are widely used and pose serious threats to human and ecosystem health. Here, palladium (Pd0)-catalyzed defluorination and microbiological mineralization were combined in a denitrifying H2-based membrane biofilm reactor to remove co-occurring perfluorooctanoic acid (PFOA) and nitrate. The combined process, i.e., Pd-biofilm, enabled continuous removal of ∼4 mmol/L nitrate and ∼1 mg/L PFOA, with 81% defluorination of PFOA. Metagenome analysis identified bacteria likely responsible for biodegradation of partially defluorinated PFOA: Dechloromonas sp. CZR5, Kaistella koreensis, Ochrobacterum anthropic, and Azospira sp. I13. High-performance liquid chromatography-quadrupole time-of-flight mass spectrometry and metagenome analyses revealed that the presence of nitrate promoted microbiological oxidation of partially defluorinated PFOA. Taken together, the results point to PFOA-oxidation pathways that began with PFOA adsorption to Pd0, which enabled catalytic generation of partially or fully defluorinated fatty acids and stepwise oxidation and defluorination by the bacteria. This study documents how combining catalysis and microbiological transformation enables the simultaneous removal of PFOA and nitrate.

Optimizing Autotrophic Sulfide Oxidation in the Oxygen-Based Membrane Biofilm Reactor to Recover Elemental Sulfur.

Biological sulfide oxidation is an efficient means to recover elemental sulfur (S0) as a valuable resource from sulfide-bearing wastewater. This work evaluated the autotrophic sulfide oxidation to S0 in the O2-based membrane biofilm reactor (O2-MBfR). High recovery of S0 (80-90% of influent S) and high sulfide oxidation (∼100%) were simultaneously achieved when the ratio of O2-delivery capacity to sulfide-to S0 surface loading (SL) (O2/S2- → S0 ratio) was around 1.5 (g O2/m2-day/g O2/m2-day). On average, most of the produced S0 was recovered in the MBfR effluent, although the biofilm could be a source or sink for S0. Shallow metagenomic analysis of the biofilm showed that the top sulfide-oxidizing genera present in all stages were Thauera, Thiomonas, Thauera_A, and Pseudomonas. Thiomonas or Pseudomonas was the most important genus in stages that produced almost only S0 (i.e., the O2/S2- → S0 ratio around 1.5 g of the O2/m2-day/g O2/m2-day). With a lower sulfide SL, the S0-producing genes were sqr and fccAB in Thiomonas. With a higher sulfide SL, the S0-producing genes were in the soxABDXYZ system in Pseudomonas. Thus, the biofilm community of the O2-MBfR adapted to different sulfide-to-S0 SLs and corresponding O2-delivery capacities. The results illustrate the potential for S0 recovery using the O2-MBfR.

Microbial Stratification Affects Conversions of Nitrogen and Methane in Biofilms Coupling Anammox and n-DAMO Processes.

A methane-based membrane biofilm reactor (MBfR) has a suitable configuration to incorporate anammox and nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO) processes because of its high gas-transfer efficiency and efficient biomass retention. In this study, the spatial distribution of microorganisms along with the biofilm depth in methane-based MBfRs was experimentally revealed, showing the dominance of anammox bacteria, n-DAMO bacteria, and n-DAMO archaea in the outer layer, middle layer, and inner layer of biofilms, respectively. The long-term and short-term experimental investigations in conjunction with mathematical modeling collectively revealed that microorganisms living in the outer layer of biofilms tend to use substrates from wastewater, while microorganisms inhabiting the inner layer of biofilms tend to use substrates originating from biofilm substratum. Specifically, anammox bacteria dominating the biofilm surface preferentially removed the nitrite provided from wastewater, while n-DAMO bacteria mostly utilized the nitrite generated from n-DAMO archaea as these two methane-related populations spatially clustered together inside the biofilm. Likewise, the methane supplied from the membrane was mostly consumed by n-DAMO archaea, while the dissolved methane in wastewater would be primarily utilized by n-DAMO bacteria. This study offers novel insights into the impacts of microbial stratification in biofilm systems, not only expanding the fundamental understanding of biofilms and microbial interactions therein but also providing a rationale for the potential applications of methane-based MBfRs in sewage treatment.

pH-dependent microbial niches succession and antibiotic resistance genes distribution in an oxygen-based membrane biofilm reactor treating greywater.

System pH is found to crucially affect biofilm growth and microorganisms' activity in the biofilm-based wastewater treatment system. This study investigated the pH-dependent pollutants removal, microbial niches succession and antibiotic resistance genes (ARGs) accumulation in an oxygen-based membrane biofilm reactor treating greywater. Results indicated that neutral conditions achieved the highest biofilm concentration and living cells, which enabled the highest pollutants removal rates; multifarious functional groups in biofilm enabled pollutants adsorption, which favored its continuous bio-removal. Microbial communities under acidic condition (pH = 5.0) were significantly different with that under other conditions (p < 0.05). The neutral and alkaline niches (pH = 7.0 and 9.0) were predominant by organics biodegradation and nitrogen reduction bacteria (e.g. Sphingobacteriales, Pseudomonas, Flavobacterium and Phenylobacterium), but which were significantly dropped under acidic conditions, leading to the declined reactor performance. ARGs in biofilm (predominant by korB, intI-1, sul1 and sul2) were much higher than that in the cell-free liquid and the target ARGs accumulation (korB, intI-1, blaCTX-M, qnrS) had nearly linear positive relationships (R2 > 0.95, P < 0.01) with biofilm-attached linear alkylbenzene sulfonate (LAS). LAS stimulate ARGs proliferation in functional microorganisms (korB, sul-1 and intI-1 were significantly associated with related microbial genus) and biofilm played a key role in ARGs dissemination. The relatively low ARGs in both biofilm and effluent under neutral conditions suggested that pH controlling can be an effective strategy to inhibit ARGs dissemination and proliferation in the system.

Surface properties of membrane materials and their role in cell adhesion and biofilm formation of microalgae.

In this study, the effects of surface properties of membrane materials on microalgae cell adhesion and biofilm formation were investigated using Chlorella vulgaris and five different types of membrane materials under hydrodynamic conditions. The results suggest that the contact angle (hydrophobicity), surface free energy, and free energy of cohesion of membrane materials alone could not sufficiently elucidate the selectivity of microalgae cell adhesion and biofilm formation on membrane materials surfaces, and membrane surface roughness played a dominant role in controlling biofilm formation rate, under tested hydrodynamic conditions. A lower level of biofilm EPS production was generally associated with a larger amount of biofilm formation. The zeta potential of membrane materials could enhance initial microalgae cell adhesion and biofilm formation through salt bridging or charge neutralization mechanisms.

Optimization of the chemolithotrophic denitrification of ion exchange concentrate using hydrogen-based membrane biofilm reactors.

A H2-based membrane biofilm reactor (MBfR) was used to remove nitrate from a synthetic ion-exchange brine made up of 23.8 g L-1 NaCl. To aid the selection of the best nitrate management strategy, our research was based on the integrated analysis of ionic exchange and MBfR processes, including a detailed cost analysis. The nitrate removal flux was not affected if key nutrients were present in the feed solution including potassium and sodium bicarbonate. Operating pH was maintained between 7 and 8. By using a H2 pressure of 15 psi, a hydraulic retention time (HRT) of 4 h, and a surface loading rate of 13.6 ± 0.2 g N m-2 d-1, the average nitrate removal flux was 3.3 ± 0.6 g N m-2 d-1. At HRTs of up to 24 h, the system was able to maintain a removal flux of 1.6 ± 0.2 g N m-2 d-1. Microbial diversity analysis showed that the consortium was dominated by the genera Sulfurimonas and Marinobacter. The estimated cost for a 200 m3/h capacity, coupled ion exchange (IX) + MBfR treatment plant is 0.43 USD/m3. This is a sustainable and competitive alternative to an IX-only plant for the same flowrate. The proposed treatment option allows for brine recycling and reduces costs by 55% by avoiding brine disposal expenses.

Dissimilatory and Cytoplasmic Antimonate Reductions in a Hydrogen-Based Membrane Biofilm Reactor.

A hydrogen-based membrane biofilm reactor (H2-MBfR) was operated to investigate the bioreduction of antimonate [Sb(V)] in terms of Sb(V) removal, the fate of Sb, and the pathways of reduction metabolism. The MBfR achieved up to 80% Sb(V) removal and an Sb(V) removal flux of 0.55 g/m2·day. Sb(V) was reduced to Sb(III), which mainly formed Sb2O3 precipitates in the biofilm matrix, although some Sb(III) was retained intracellularly. High Sb(V) loading caused stress that deteriorated performance that was not recovered when the high Sb(V) loading was removed. The biofilm community consisted of DSbRB (dissimilatory Sb-reduction bacteria), SbRB (Sb-resistant bacteria), and DIRB (dissimilatory iron-reducing bacteria). Dissimilatory antimonate reduction, mediated by the respiratory arsenate reductase ArrAB, was the main reduction route, but respiratory reduction coexisted with cytoplasmic Sb(V)-reduction mediated by arsenate reductase ArsC. Increasing Sb(V) loading caused stress that led to increases in the expression of arsC gene and intracellular accumulation of Sb(III). By illuminating the roles of the dissimilatory and cytoplasmic Sb(V) reduction mechanism in the biofilms of the H2-MBfR, this study reveals that the Sb(V) loading should be controlled to avoid stress that deteriorates Sb(V) reduction.

Carboxylates and alcohols production in an autotrophic hydrogen-based membrane biofilm reactor.

Microbiological conversion of CO2 into biofuels and/or organic industrial feedstock is an excellent carbon-cycling strategy. Here, autotrophic anaerobic bacteria in the membrane biofilm reactor (MBfR) transferred electrons from hydrogen gas (H2 ) to inorganic carbon (IC) and produced organic acids and alcohols. We systematically varied the H2 -delivery, the IC concentration, and the hydraulic retention time in the MBfR. The relative availability of H2 versus IC was the determining factor for enabling microbial chain elongation (MCE). When the H2 :IC mole ratio was high (>2.0 mol H2 /mol C), MCE was an important process, generating medium-chain carboxylates up to octanoate (C8, 9.1 ± 1.3 mM C and 28.1 ± 4.1 mmol C m-2 d-1 ). Conversely, products with two carbons were the only ones present when the H2 :IC ratio was low (<2.0 mol H2 /mol C), so that H2 was the limiting factor. The biofilm microbial community was enriched in phylotypes most similar to the well-known acetogen Acetobacterium for all conditions tested, but phylotypes closely related with families capable of MCE (e.g., Bacteroidales, Rhodocyclaceae, Alcaligenaceae, Thermoanaerobacteriales, and Erysipelotrichaceae) became important when the H2 :IC ratio was high. Thus, proper management of IC availability and H2 supply allowed control over community structure and function, reflected by the chain length of the carboxylates and alcohols produced in the MBfR.

Three-dimension oxygen gradient induced low energy input for grey water treatment in an oxygen-based membrane biofilm reactor.

Grey water (GW) containing high levels of linear alkylbenzene sulfonates (LAS) can be a threat to human health and organisms in the environment if not treated properly. Although aerobic treatment could achieve high organics removal efficiency, conventional aeration can lead to serious foaming and energy waste. Here, we systematically evaluated an oxygen based membrane biofilm reactor (O2-MBfR) for its capacity to simultaneously remove organics and nitrogen from GW. The dissolved oxygen (DO) concentration inside the reactor was maintained at 0.4 mg/L by gradually controlling the lumen air pressure. Results showed that the O2-MBfR achieved high removal efficiency of total chemical oxygen demand (TCOD), total linear alkylbenzene sulfonates (LAS) and total nitrogen (TN) of 89.7%, 99.1% and 78.1%, respectively, with a hydraulic retention time (HRT) of 7.5 h. Lower HRT (7.0 h) led to the accumulation of LAS in the biofilm, which caused cell lysis and damaged the O2-MBfR system, leading to a discernible and continuous decline of the reactor performance. The O2-MBfR design completely eliminated foaming formation and the three-dimension oxygen gradient design led to low air pressure inside the membrane fiber, which enabled the high removal efficiency for both organics and nitrogen with low energy input and GW treatment cost, providing the fundamental knowledge for practical application of O2-MBfR in wastewater treatment.

Enhancing mainstream nitrogen removal by employing nitrate/nitrite-dependent anaerobic methane oxidation processes.

Due to serious eutrophication in water bodies, nitrogen removal has become a critical stage for wastewater treatment plants (WWTPs) over past decades. Conventional biological nitrogen removal processes are based on nitrification and denitrification (N/DN), and are suffering from several major drawbacks, including substantial aeration consumption, high fugitive greenhouse gas emissions, a requirement for external carbon sources, excessive sludge production and low energy recovery efficiency, and thus unable to satisfy the escalating public needs. Recently, the discovery of anaerobic ammonium oxidation (anammox) bacteria has promoted an update of conventional N/DN-based processes to autotrophic nitrogen removal. However, the application of anammox to treat domestic wastewater has been hindered mainly by unsatisfactory effluent quality with nitrogen removal efficiency below 80%. The discovery of nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) during the last decade has provided new opportunities to remove this barrier and to achieve a robust system with high-level nitrogen removal from municipal wastewater, by utilizing methane as an alternative carbon source. In the present review, opportunities and challenges for nitrate/nitrite-dependent anaerobic methane oxidation are discussed. Particularly, the prospective technologies driven by the cooperation of anammox and n-DAMO microorganisms are put forward based on previous experimental and modeling studies. Finally, a novel WWTP system acting as an energy exporter is delineated.

Kinetics of anaerobic methane oxidation coupled to denitrification in the membrane biofilm reactor.

Anaerobic oxidation of methane coupled to denitrification (AOM-D) in a membrane biofilm reactor (MBfR), a platform used for efficiently coupling gas delivery and biofilm development, has attracted attention in recent years due to the low cost and high availability of methane. However, experimental studies have shown that the nitrate-removal flux in the CH4 -based MBfR (<1.0 g N/m2 -day) is about one order of magnitude smaller than that in the H2 -based MBfR (1.1-6.7 g N/m2 -day). A one-dimensional multispecies biofilm model predicts that the nitrate-removal flux in the CH4 -based MBfR is limited to <1.7 g N/m2 -day, consistent with the experimental studies reported in the literature. The model also determines the two major limiting factors for the nitrate-removal flux: The methane half-maximum-rate concentration (K2 ) and the specific maximum methane utilization rate of the AOM-D syntrophic consortium (kmax2 ), with kmax2 being more important. Model simulations show that increasing kmax2 to >3 g chemical oxygen demand (COD)/g cell-day (from its current 1.8 g COD/g cell-day) and developing a new membrane with doubled methane-delivery capacity (Dm ) could bring the nitrate-removal flux to ≥4.0 g N/m2 -day, which is close to the nitrate-removal flux for the H2 -based MBfR. Further increase of the maximum nitrate-removal flux can be achieved when Dm and kmax2 increase together.

Insights into selenate removal mechanism of hydrogen-based membrane biofilm reactor for nitrate-polluted groundwater treatment based on anaerobic biofilm analysis.

The selenate removal mechanism of hydrogen-based membrane biofilm reactor (MBfR) for nitrate-polluted groundwater treatment was studied based on anaerobic biofilm analysis. A laboratory-scale MBfR was operated for over 60 days with electron balance, structural analysis, and bacterial community identification. Results showed that anaerobic biofilm had an excellent removal of both selenate (95%) and nitrate (100%). Reduction of Selenate → Selenite → Se0 with hydrogen was the main pathway of anaerobic biofilm for selenate removal with amorphous Se0 precipitate accumulating in the biofilm. The element selenium was observed to be evenly distributed along the cross-sectional thin biofilm. A part of selenate (3%) was also reduced into methyl-selenide by heterotrophic bacteria. Additionally, Hydrogenophaga bacteria of ß-Proteobacteria, capable of both nitrate and selenate removal, worked as the dominant species (over 85%) in the biofilm and contributed to the stable removal of both nitrate and selenate. With the selenate input, bacteria with a capacity for both selenate and nitrate removal were also developed in the anaerobic biofilm community.

Simultaneous removal of elemental mercury and NO by mercury induced thermophilic community in membrane biofilm reactor.

Thermophilic membrane biofilm reactor (TMBR) for elemental mercury (Hg0) and NO removal in simulated flue gas was investigated at oxygen content of 6% and 60 °C. The performance, the microbial community structures, gene function and the mechanism for Hg0 and NO removal in the TMBR were evaluated. TMBR achieved effective simultaneous Hg0 and NO removal in 210 days of operation, Hg0 and NO removal efficiency were up to 88.9% and 85.3%, respectively. Mercury induced thermophilic community had been formed significantly. Comamonas, Pseudomonas, Desulfomicrobium, Burkholderia and Halomonas were thermophilic mercury resistant bacteria. Brucella, Paracoccus, Tepidiphilus, Proteobacteria, Pseudomonas and Symbiobacterium were nitrifying/denitrifying genera, and had functional genes of mercury and nitrogen metabolism, as shown by16S rDNA and metagenomic sequencing. The biofilm in TMBR was characterized by XPS, HPLC. XPS and HPLC spectra indicate the formation of a mercuric species (Hg2+) from mercury oxidation. TMBR used oxygen as electron acceptor, NO and Hg0 as electron donor in nitrification; O2, NO and NO3- could be used as electron acceptor and Hg0 as electron donor in denitrification.

Simultaneous enrichment of denitrifying anaerobic methane-oxidizing microorganisms and anammox bacteria in a hollow-fiber membrane biofilm reactor.

In this study, the coculture system of denitrifying anaerobic methane oxidation (DAMO) microbes and anaerobic ammonium oxidation (anammox) bacteria was successfully enriched in a hollow-fiber membrane biofilm reactor (HfMBR) using freshwater sediment as the inoculum. The maximal removal rates of nitrate and ammonium were 78 mg N/L/day (131 mg N/m2/day) and 26 mg N/L/day (43 mg N/m2/day), respectively. Due to the high rate of methane mass transfer in HfMBR, the activity of DAMO archaea continued to increase during the enrichment period, indicating that HfMBR could be a powerful tool to enrich DAMO microorganisms. Effects of partial methane pressure, temperature, and pH on the cocultures were obvious. However, the microbial activity in HfMBR could be recovered quickly after the shock change of environmental factors. Furthermore, the result also found that DAMO bacteria likely had a stronger competitive advantage than anammox bacteria under the operating conditions in this study. High-throughput sequencing 16S rRNA genes illustrated that the dominant microbes were NC10, Euryarchaeota, Proteobacteria, Planctomycetes, and Chlorobi with relative abundance of 38.8, 26.2, 13.78, 6.2, and 3.6 %, respectively.

Enhancement of acetate productivity in a thermophilic (55 °C) hollow-fiber membrane biofilm reactor with mixed culture syngas (H2/CO2) fermentation.

Conversion of organic wastes to syngas is an attractive way to utilize wastes. The produced syngas can be further used to produce a variety of chemicals. In this study, a hollow-fiber membrane biofilm reactor with mix cultures was operated at 55 °C to convert syngas (H2/CO2) into acetate. A high concentration of acetate (42.4 g/L) was reached in batch experiment while a maximum acetate production rate of 10.5 g/L/day was achieved in the continuous-flow mode at hydraulic retention time (HRT) of 1 day. Acetate was the main product in both batch and continuous-flow experiments. n-Butyrate was the other byproduct in the reactor. Acetate accounted for more than 98.5 and 99.1% of total volatile fatty acids in batch and continuous modes, respectively. Illumina Miseq high-throughput sequencing results showed that microorganisms were highly purified and enriched in the reactor. The main genus was Thermoanaerobacterium (66% of relative abundance), which was usually considered as H2 producer in the literature, however, likely played a role as a H2 consumer in this study. This study provides a new method to generate the high producing rate and purity of acetate from syngas.

Metagenomics coupled with thermodynamic analysis revealed a potential way to improve the nitrogen removal efficiency of the aerobic methane oxidation coupled to denitrification process under the hypoxic condition.

Aerobic methane (CH4) oxidation coupled to denitrification (AME-D) is a promising wastewater treatment process for CH4 utilization and nitrogen removal. However, it is unclear which CH4-derived carbons are suitable for the AME-D process and how these organics are metabolized. In this study, metagenomics coupled with a thermodynamic model were used to explore the microorganisms and their metabolic mechanisms in an AME-D membrane biofilm reactor (MBfR) with high nitrogen removal efficiency. Results revealed that the aerobic methanotrophs of Methylomonas with the CH4-based fermentation potential were highly enriched and played an important role in CH4 conversion in the MBfR. Bacteria of Xanthomonadaceae, Methylophilaceae, Bacteroidetes, Rhodocyclaceae, Hyphomicrobium were the main denitrifiers. C1 compounds (methanol, formaldehyde and formate) and CH4-based fermentation products are promising cross-feeding intermediates of the AME-D. Specially, by means of integrating the CH4-based fermentation with denitrification, the minimum amount of CH4 required to remove per mole of nitrate can be further reduced to 1.25 mol-CH4 mol-1-NO3-, even lower than that of methanol. Compared to the choice to secrete methanol, type I aerobic methanotrophs require a 15 % reduction in the amount of oxygen required to secrete fermentation metabolites, but a 72 % increase in the amount of CH4-C released. Based on this trade-off, optimizing oxygen supply strategies will help to construct engineered microbiomes focused on aerobic methanotrophs with CH4-based fermentation potential. This study gives an insight into C and N conversions in the AME-D process and highlights the role of CH4-based fermentation in improving the nitrogen removal efficiency of the AME-D process.

Reduction and precipitation of chromium(VI) using a palladized membrane biofilm reactor.

H2-driven reduction of hexavalent chromium (Cr(VI)) using precious-metal catalysts is promising, but its implementation in water treatment has been restricted by poor H2-transfer efficiency and high catalyst loss. We investigated the reduction of Cr(VI) through hydrogenation catalyzed by elemental-palladium nanoparticles (PdNPs) generated in-situ within biofilm of a membrane biofilm reactor (MBfR), creating a Pd-MBfR. Experiments were conducted using a Pd-MBfR and a non-Pd MBfR. The Pd-MBfR achieved Cr(VI) (1000 µg L-1) reduction of >99 % and reduced the concentration of total Cr to below 50 µg L-1, much lower than the total Cr concentration in the non-Pd MBfR effluent (290 µg L-1). The Pd-MBfR also had a lower concentration of dissolved organic compounds compared to the non-Pd MBfR, which minimized the formation of soluble organo-Cr(III) complexes and promoted precipitation of Cr(OH)3. Solid-state characterizations documented deposition of Cr(OH)3 as the product of Cr(VI) reduction in the Pd-MBfR. Metagenomic analyses revealed that the addition and reduction of Cr(VI) had minimal impact on the microbial community (dominated by Dechloromonas) and functional genes in the biofilm of the Pd-MBfR, since the PdNP-catalyzed reduction process was rapid. This study documented efficient Cr(VI) reduction and precipitation of Cr(OH)3 by the Pd-MBfR technology.

Treatment of volatile organic compounds and other waste gases using membrane biofilm reactors: A review on recent advancements and challenges.

This article provides a comprehensive review of membrane biofilm reactors for waste gas (MBRWG) treatment, focusing on studies conducted since 2000. The first section discusses the membrane materials, structure, and mass transfer mechanism employed in MBRWG. The concept of a partial counter-diffusion biofilm in MBRWG is introduced, with identification of the most metabolically active region. Subsequently, the effectiveness of these biofilm reactors in treating single and mixed pollutants is examined. The phenomenon of membrane fouling in MBRWG is characterized, alongside an analysis of contributory factors. Furthermore, a comparison is made between membrane biofilm reactors and conventional biological treatment technologies, highlighting their respective advantages and disadvantages. It is evident that the treatment of hydrophobic gases and their resistance to volatility warrant further investigation. In addition, the emergence of the smart industry and its integration with other processes have opened up new opportunities for the utilization of MBRWG. Overcoming membrane fouling and developing stable and cost-effective membrane materials are essential factors for successful engineering applications of MBRWG. Moreover, it is worth exploring the mechanisms of co-metabolism in MBRWG and the potential for altering biofilm community structures.

Cooperation and competition between denitrification and chromate reduction in a hydrogen-based membrane biofilm reactor.

Competition and cooperation between denitrification and Cr(VI) reduction in a H2-based membrane biofilm reactor (H2-MBfR) were documented over 55 days of continuous operation. When nitrate (5 mg N/L) and chromate (0.5 mg Cr/L) were fed together, the H2-MBfR maintained approximately 100 % nitrate removal and 60 % chromate Cr(VI) removal, which means that nitrate outcompeted Cr(VI) for electrons from H2 oxidation. Removing nitrate from the influent led to an immediate increase in Cr(VI) removal (to 92 %), but Cr(VI) removal gradually deteriorated, with the removal ratio dropping to 14 % after five days. Cr(VI) removal resumed once nitrate was again added to the influent. 16S rDNA analyses showed that bacteria able to carry out H2-based denitrification and Cr(VI) reduction were in similar abundances throughout the experiment, but gene expression for Cr(VI)-reduction and export shifted. Functional genes encoding for energy-consuming chromate export (encoded by ChrA) as a means of bacterial resistance to toxicity were more abundant than genes encoding for the energy producing Cr(VI) respiration via the chromate reductase ChrR-NdFr. Thus, Cr(VI) transport and resistance to Cr(VI) toxicity depended on H2-based denitrification to supply energy. With Cr(VI) being exported from the cells, Cr(VI) reduction to Cr(III) was sustained. Thus, cooperation among H2-based denitrification, Cr(VI) export, and Cr(VI) reduction led to sustained Cr(VI) removal in the presence of nitrate, even though Cr(VI) reduction was at a competitive disadvantage for utilizing electrons from H2 oxidation.