RESUMEN
A novel furanosteroid named 9-epi-viridiol (1), along with viridiol (2) and mevalonic acid (3), was isolated from Trichoderma virens. The structure of 1 was verified by combined spectroscopic data (COSY, HSQC, HMBC and NOESY) to be a C-9 epimer of viridiol. 9-epi-Viridiol exhibited cytotoxicity towards HeLa and KB cells with IC50 values of 19 and 50 microg mL(-1), respectively.
Asunto(s)
Androstenodioles/química , Antineoplásicos/química , Trichoderma/química , Androstenodioles/aislamiento & purificación , Androstenodioles/farmacología , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Ensayos de Selección de Medicamentos Antitumorales , Formazáns , Células HeLa , Humanos , Concentración 50 Inhibidora , Células KB , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Rotación Óptica , Espectrofotometría Ultravioleta , Estereoisomerismo , Espectrometría de Masas en Tándem , Sales de Tetrazolio , TailandiaRESUMEN
An improved method for the separation of epimeric C19O2 steroids and their related allylic alcohols is described. In this method, the steroids are first separated by over-run thin-layer chromatography, and the unresolved groups are further analysed as free or as trimethylsilyl ether derivatives by gas-liquid chromatography. The behaviour of twenty-one C19O2 steroids was investigated by thin-layer chromatography in four systems and by gas-liquid chromatography in four liquid phases. All steroid pairs of similar polarity were resolved by the combination of these two fractionation procedures.