eSoil: A low-power bioelectronic growth scaffold that enhances crop seedling growth.

Active hydroponic substrates that stimulate on demand the plant growth have not been demonstrated so far. Here, we developed the eSoil, a low-power bioelectronic growth scaffold that can provide electrical stimulation to the plants' root system and growth environment in hydroponics settings. eSoil's active material is an organic mixed ionic electronic conductor while its main structural component is cellulose, the most abundant biopolymer. We demonstrate that barley seedlings that are widely used for fodder grow within the eSoil with the root system integrated within its porous matrix. Simply by polarizing the eSoil, seedling growth is accelerated resulting in increase of dry weight on average by 50% after 15 d of growth. The effect is evident both on root and shoot development and occurs during the growth period after the stimulation. The stimulated plants reduce and assimilate NO3- more efficiently than controls, a finding that may have implications on minimizing fertilizer use. However, more studies are required to provide a mechanistic understanding of the physical and biological processes involved. eSoil opens the pathway for the development of active hydroponic scaffolds that may increase crop yield in a sustainable manner.

Development of a method for the simultaneous determination of ionic nutrients in hydroponic solutions using cation-/anion-exchange chromatography with a neutral eluent.

Nutrient availability in hydroponic solutions must be accurately monitored to maintain crop productivity; however, few cost-effective, accurate, real-time, and long-term monitoring technologies have been developed. In this study, we describe the development and application of cation-/anion-exchange chromatography with a neutral eluent (20-mmol/L sodium formate, pH 7.87) for the simultaneous separation (within 50 min) of ionic nutrients, including K+, NH4+, NO2-, NO3-, and phosphate ion, in a hydroponic fertilizer solution. Using the neutral eluent avoided degradation of the separation column during precipitation of metal ion species, such as hydroxides, with an alkaline eluent and oxidation of NO2- to NO3- with an acidic eluent. The suitability of the current method for monitoring ionic components in a hydroponic fertilizer solution was confirmed. Based on our data, we propose a controlled fertilizer strategy to optimize fertilizer consumption and reduce the chemical load of drained fertilizer solutions.

The effect of swimming activity and feed restriction of rainbow trout (Oncorhynchus mykiss) on water quality and fish-plant growth performance in aquaponics.

In this study, we investigated the effects of swimming activity and feed restriction on juvenile rainbow trout (Oncorhynchus mykiss) in decoupled aquaponic systems. Our focus was on assessing their impact on water quality parameters within the aquaponic setup and evaluating the growth performance of the fish, including final weight (FW), condition factor (K), coefficient of variation (c.v.) in weight, specific growth rate (SGR), total feed intake (g/fish), feed conversion rate (FCR), hepatosomatic index (HSI), and viscerosomatic index (VSI), as well as the growth of lettuce (Lactuca sativa L. var. elmaria). The study involved 108 juvenile rainbow trout with an average initial weight of 26.54 ± 0.36 g and 60 ten-day-old lettuce seedlings, over a period of 42 days. We designed four treatment groups, each with three fish tanks: static ad libitum (SA), where fish were in static water conditions and fed to satiation; static restriction (SR), with fish in static water and a 25% feed restriction; current ad libitum (CA), where fish experienced forced swimming at 1 BL s-1; and current restriction (CR), with swimming exercise at 1 BL s-1 and a 25% feed restriction. Using a flow rate of 1 BL s-1 in the tanks for rainbow trout yielded several benefits. Notably, the fish in the CA group exhibited increased feed intake (60 ± 1.78 g fish-1) and enhanced fish growth with an FW of 91.72 ± 0.91 g, compared to the SA group (55.88 ± 0.88 g fish-1 for feed intake and 89.26 ± 0.81 g for FW). In contrast, the CR group showed a reduced feed intake (39.02 ± 2.78 g fish-1) and a lower FW (67.85 ± 1.49 g) compared to the CA group. In addition, the CA group demonstrated positive contributions to fish development with a reduced HSI (1.26 ± 0.02) in comparison to the SA group (1.56 ± 0.14). Inadequate nutrient provisioning in the SR and CR groups negatively impacted fish growth and system efficiency. Our findings suggest that optimizing water flow and feed benefits fish and plants and enhances system sustainability.

ROS regulated reversible protein phase separation synchronizes plant flowering.

How aerobic organisms exploit inevitably generated but potentially dangerous reactive oxygen species (ROS) to benefit normal life is a fundamental biological question. Locally accumulated ROS have been reported to prime stem cell differentiation. However, the underlying molecular mechanism is unclear. Here, we reveal that developmentally produced H2O2 in plant shoot apical meristem (SAM) triggers reversible protein phase separation of TERMINATING FLOWER (TMF), a transcription factor that times flowering transition in the tomato by repressing pre-maturation of SAM. Cysteine residues within TMF sense cellular redox to form disulfide bonds that concatenate multiple TMF molecules and elevate the amount of intrinsically disordered regions to drive phase separation. Oxidation triggered phase separation enables TMF to bind and sequester the promoter of a floral identity gene ANANTHA to repress its expression. The reversible transcriptional condensation via redox-regulated phase separation endows aerobic organisms with the flexibility of gene control in dealing with developmental cues.

Comparative analysis of IoT-based controlled environment and uncontrolled environment plant growth monitoring system for hydroponic indoor vertical farm.

Agriculture is a very important economic sector that contributes to a nation's overall economic development. According to the UN Food and Agriculture Organization, with a rising population, the cereals demand will reach 3 billion tons by 2050. Also, the mission of Sustainable Development Goals (SDGs) is to provide zero hunger and sustainable agriculture by 2030. With a simultaneous decline in cultivable land and water scarcity, food production has to increase in order to achieve the above mission. Vertical farming is a current state of art agriculture technology to increase crop yield per unit area. This work focuses on designing and constructing an IoT-enabled smart vertical farming system with a controlled environment for plant growth. This system uses the hydroponic Deep Flow Technique (DFT), various sensors, and an auto pH and Total Dissolved Solids (TDS) balancing system. This paper provides a comparative analysis of IoT-based controlled environment vertical farming setup with the uncontrolled setup for Romaine lettuce in terms of plant growth parameters like plant height, maximum leaf length, maximum leaf width, and fresh and dry weight of the plant. The observed fresh weight of the aerial part for automated and unautomated setup is found to be 58.66 g and 48.81 g respectively. Also, the chemical analysis showed the plants possess the required optimum range of micro and macronutrients for both setups. The macronutrient results obtained for the controlled/automated (A setup), and uncontrolled/unautomated setup (U setup for Phosphorus (P), and Potassium (K) are (PA, PU) (5.91 g/Kg, 6.06 g/Kg), and (KA, KU) (67.03 g/Kg, 74.01 g/Kg) respectively.

Nutrient Solution Flowing Environment Affects Metabolite Synthesis Inducing Root Thigmomorphogenesis of Lettuce (Lactuca sativa L.) in Hydroponics.

The principal difference between hydroponics and other substrate cultivation methods is the flowing liquid hydroponic cultivation substrate. Our previous studies have revealed that a suitable flowing environment of nutrient solution promoted root development and plant growth, while an excess flow environment was unfavorable for plants. To explain the thigmomorphogenetic response of excess flow-induced metabolic changes, six groups of lettuce (Lactuca sativa L.), including two flow conditions and three time periods, were grown. Compared with the plants without flow, the plants with flow showed decreased root fresh weight, total root length, root surface area, and root volume but increased average root diameter and root density. The roots with flow had more upregulated metabolites than those without flow, suggesting that the flow may trigger metabolic synthesis and activity. Seventy-nine common differential metabolites among six groups were screened, and enrichment analysis showed the most significant enrichment in the arginine biosynthesis pathway. Arginine was present in all the groups and exhibited greater concentrations in roots with flow than without flow. It can be speculated from the results that a high-flowing environment of nutrient solution promotes arginine synthesis, resulting in changes in root morphology. The findings provide insights on root thigmomorphogenesis affected by its growing conditions and help understand how plants respond to environmental mechanical forces.

Urine and grey water based liquid fertilizer - Production and the response of plants.

Plant cultivation is a key aspect of future long-distance space missions, and the creation of an efficient food system will not be possible without it. The production of fertilizer in space is based on the recovery of water and nutrients from wastewater, such as urine and grey water. In this study, the fertilizer production process was conducted in an aerobic, activated sludge reactor, where nitrification and the process of carbon removal take place. Treated streams have three potential factors that could affect the plants growth in a hydroponic system (anionic surfactants, nutrients deficiencies, high salinity). The effect of these factors was examined for two hydroponic configurations. Their influence on lettuce yield, quality parameters and stress response were investigated and compared to the control cultivation. The results showed that the main cause of a decrease (up to 24%) in the yield productivity of plants grown on nitrified urine and grey water is oxidative stress originated from a deficiency of elements, not from used anionic surfactant. Enrichment with nutrients resulted in the restoration of proper protein synthesis and an increase in the activity of antioxidant enzymes, which was positively reflected in the qualitative and quantitative parameters of the enriched cultivation (fresh leaves mass equal to 103% of the control). Results also show that Sodium Methyl Cocoyl Taurate (SMCT) surfactant itself after biological treatment used in plant cultivation has no negative effects reflected in lettuce yield or quality.

Digital Twinning of Hydroponic Grow Beds in Intelligent Aquaponic Systems.

The use of automation, Internet-of-Things (IoT), and smart technologies is being rapidly introduced into the development of agriculture. Technologies such as sensing, remote monitoring, and predictive tools have been used with the purpose of enhancing agriculture processes, aquaponics among them, and improving the quality of the products. Digital twinning enables the testing and implementing of improvements in the physical component through the implementation of computational tools in a 'twin' virtual environment. This paper presents a framework for the development of a digital twin for an aquaponic system. This framework is validated by developing a digital twin for the grow beds of an aquaponics system for real-time monitoring parameters, namely pH, electroconductivity, water temperature, relative humidity, air temperature, and light intensity, and supports the use of artificial intelligent techniques to, for example, predict the growth rate and fresh weight of the growing crops. The digital twin presented is based on IoT technology, databases, a centralized control of the system, and a virtual interface that allows users to have feedback control of the system while visualizing the state of the aquaponic system in real time.

Phytochemicals and quality level of food plants grown in an aquaponics system.

BACKGROUND: Beyond nutrition, fruits and vegetables can be considered as natural sources of bioactive molecules, for which beneficial effects on human health are widely recognised. To improve food quality, soilless growing systems could represent a good strategy for promoting a sustainable food production chain, although the nutritional and nutraceutical properties of their products should be investigated in depth. The main quality traits and the volatile and non-volatile secondary metabolites of Solanum lycopersicum L., Petroselinum crispum (Mill.) Fuss and Ocimun basilicum L. grown in an aquaponics system and in organic farming were quantified and compared. RESULTS: On a fresh basis, soil-grown P. crispum and O. basilicum showed significantly higher total phenolics and antioxidant activity compared to aquaponic crops, whereas, on a dry basis, both plants showed opposite results. Soil-grown S. lycopersicum was significantly richer in total phenolics, whereas the aquaponic type showed a higher antioxidant activity. Aquaponics induced the accumulation of resveratrol in P. crispum, rosmarinic acid and myricetin in O. basilicum, and lycopene in S. lycopersicum. Among the volatile compounds, in O. basilicum, linalool was the main constituent in both treatments, whereas τ-cadinol represented the second constituent in aquaponic crops. The volatile profiles of P. crispum did not differ significantly between the two cultivation methods. CONCLUSION: The overall quality of organic and aquaponics cultures appeared to be comparable. The results showed that aquaponic farming method can be an innovative, rapid and sustainable way of producing quality food. © 2021 Society of Chemical Industry.

Enhancement of developmentally regulated daidzein secretion from soybean roots in field conditions as compared with hydroponic culture.

Analyses of metabolite secretions by field-grown plants remain scarce. We analyzed daidzein secretion by field-grown soybean. Daidzein secretion was higher during early vegetative stages than reproductive stages, a trend that was also seen for hydroponically grown soybean. Daidzein secretion was up to 10 000-fold higher under field conditions than hydroponic conditions, leading to a more accurate simulation of rhizosphere daidzein content.

Effects of Supplemental Lighting on Potassium Transport and Fruit Coloring of Tomatoes Grown in Hydroponics.

Supplemental blue/red lighting accelerated fruit coloring and promoted lycopene synthesis in tomato fruits. Potassium (K) is the most enriched cation in tomato fruits, and its fertigation improved tomato yield and fruit color. However, the effects of supplemental lighting on K uptake and transport by tomatoes and whether supplemental lighting accelerates fruit coloring through enhancing K uptake and transport are still unclear. We investigated the effects of supplemental light-emitting diode (LED) lighting (SL; 100% red, 100% blue; 75% red combined 25% blue) on K uptake in roots and transport in the fruits as well as the fruit coloring of tomatoes (Micro-Tom) grown in an experimental greenhouse in hydroponics. The use of red SL or red combined blue SL enhanced K uptake and K accumulation as well as carotenoid (phytoene, lycopene, γ-carotene, and ß-carotene) content in fruits by increasing photosynthesis, plant growth, and fruit weight. The genes related to ethylene signaling were upregulated by red SL. Quantitative real-time PCR (qRT-PCR) results showed that K transporter genes (SlHAKs) are differentially expressed during fruit development and ripening. The highest-expressed gene was SlHAK10 when fruit reached breaker and ripening. SlHAK3 and SlHAK19 were highly expressed at breaker, and SlHAK18 was highly expressed at ripening. These might be related to the formation of tomato fruit ripening and quality. SlHAK4, SlHAK6, SlHAK8, and SlHAK9 were significantly downregulated with fruit ripening and induced by low K. The expression level of SlHAK6, SlHAK10, SlHAK15, and SlHAK19 were significantly increased by blue SL or red combined blue SL during breaker and ripening. Blue SL or red combined blue SL increased content of phytoene, ß-carotene, α-carotene, and γ-carotene and accelerated fruit coloring by enhancing K uptake in roots and transport in fruits during fruit ripening. This was consistent with the expression level of SlHAK6, SlHAK10, SlHAK15, and SlHAK19 during fruit development and ripening. The key genes of photoreceptors, light signaling transcript factors as well as abscisic acid (ABA) transduction induced by blue SL or red combined blue SL were consistent with the upregulated genes of SlHAK6, SlHAK10, SlHAK15, and SlHAK19 under blue SL and red combined blue SL. The K transport in tomato fruits might be mediated by light signaling and ABA signaling transduction. These results provide valuable information for fruit quality control and the light regulating mechanism of K transport and fruit coloring in tomatoes.

Chemical Composition and Cytotoxic Activity of the Essential Oil and Oleoresins of In Vitro Micropropagated Ansellia africana Lindl: A Vulnerable Medicinal Orchid of Africa.

Orchids are rich treasure troves of various important phytomolecules. Among the various medicinal orchids, Ansellia africana stands out prominently in the preparing of various herbal medicines due to its high therapeutic importance. The nodal explants of A. africana were sampled from asymbiotically germinated seedlings on basal Murashige and Skoog (MS) medium and were micropropagated in MS medium supplemented with 3% sucrose and 10 µM meta topolin (mT) + 5 µM naphthalene acetic acid (NAA) +15 µM indole butyric acid (IBA) + 30 µM phloroglucinol (PG). In the present study, the essential oil was extracted by hydrodistillation and the oleoresins by the solvent extraction method from the micropropagated A. africana. The essential oil and the oleoresins were analysed by Gas Chromatography (GC) and GC/MS (Mass spectrometry). A total of 84 compounds were identified. The most predominant components among them were linoleic acid (18.42%), l-ascorbyl 2,6-dipalmitate (11.50%), linolenic acid (10.98%) and p-cresol (9.99%) in the essential oil; and eicosane (26.34%), n-butyl acetate (21.13%), heptadecane (16.48%) and 2-pentanone, 4-hydroxy-4-methyl (11.13%) were detected in the acetone extract; heptadecane (9.40%), heneicosane (9.45%), eicosane (6.40%), n-butyl acetate (14.34%) and styrene (22.20%) were identified and quantified in the ethyl acetate extract. The cytotoxic activity of essential oil and oleoresins of micropropagated A. africana was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide) assay on Vero cells compared to the standard drug doxorubicin chloride. The present research contains primary information about the therapeutic utility of the essential oil and oleoresins of A. africana with a promising future research potential of qualitative and quantitative improvement through synchronised use of biotechnological techniques.

Zinc Oxide Nanoparticles Alleviate Chilling Stress in Rice (Oryza Sativa L.) by Regulating Antioxidative System and Chilling Response Transcription Factors.

As one of the common abiotic stresses, chilling stress has negative effects on rice growth and development. Minimization of these adverse effects through various ways is vital for the productivity of rice. Nanoparticles (NPs) serve as one of the effective alleviation methods against abiotic stresses. In our research, zinc oxide (ZnO) NPs were utilized as foliar sprays on rice leaves to explore the mechanism underlying the effect of NPs against the negative impact of chilling stress on rice seedlings. We revealed that foliar application of ZnO NPs significantly alleviated chilling stress in hydroponically grown rice seedlings, including improved plant height, root length, and dry biomass. Besides, ZnO NPs also restored chlorophyll accumulation and significantly ameliorated chilling-induced oxidative stress with reduced levels of H2O2, MDA, proline, and increased activities of major antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). We further found that foliar application of ZnO NPs induced the chilling-induced gene expression of the antioxidative system (OsCu/ZnSOD1, OsCu/ZnSOD2, OsCu/ZnSOD3, OsPRX11, OsPRX65, OsPRX89, OsCATA, and OsCATB) and chilling response transcription factors (OsbZIP52, OsMYB4, OsMYB30, OsNAC5, OsWRKY76, and OsWRKY94) in leaves of chilling-treated seedlings. Taken together, our results suggest that foliar application of ZnO NPs could alleviate chilling stress in rice via the mediation of the antioxidative system and chilling response transcription factors.

Controlled root-zone temperature effect on baby leaf vegetables yield and quality in a floating system under mild and extreme weather conditions.

BACKGROUND: A floating system is a suitable low-cost hydroponic method for growing baby leaf vegetables. Among other, an important characteristic of the system is the use of large volume of nutrient solution which is characterized by high heat capacity. The aim of this study was to evaluate the effect of different root-zone temperatures on baby leaves of lettuce and rocket plants grown in a floating system under mild (spring) or extreme environmental conditions (summer and winter). RESULTS: Root-zone temperature was recorded in two tanks, one powered by a photovoltaic system and one where root-zone temperature was not controlled - this was used as a control tank. Photosynthetic parameters, yield, nutritional quality, and mineral composition were determined. In both baby leaf vegetables, during extreme weather conditions, yield was higher in the tanks with controlled root-zone temperature conditions than the control (+18.9% for rocket, and + 31.4% for baby lettuce), while quality parameters and chemical composition were not significantly affected. Stomatal conductance and net photosynthesis values were positively affected only during summer. On the other hand, control of root-zone temperature under mild weather conditions had no significant effect on baby lettuce and rocket. CONCLUSION: Control of the root-zone temperature could be a useful tool to improve productivity for baby lettuce and rocket crops cultivated in floating systems under extreme weather conditions. © 2020 Society of Chemical Industry.

Establishment of a cultivation method for leaf lettuce (Lactuca sativa var. crispa) and komatsuna (Brassica rapa var. perviridis) with high zinc content for patients with zinc deficiency and evaluation of its effectiveness.

BACKGROUND: Insufficient intake of zinc is associated with various diseases worldwide. To overcome this problem, we aimed to establish a method for cultivating leafy vegetables with high zinc content in hydroponics without inhibiting their growth. Furthermore, we evaluated the effectiveness of the cultivated leafy vegetables with high zinc content in zinc-deficient mice. RESULTS: By adjusting the zinc concentration in the hydroponic solution to 5 mg L-1 starting from 7 days before harvesting, the zinc content in leaf lettuce increased eight times of that in the control, without any inhibition of the growth. Furthermore, when oxidized glutathione (GSSG) was added simultaneously with zinc to the hydroponic solution, the zinc content further doubled (16 times of that in the control). Similar results were obtained with komatsuna and red leaf lettuce, although there was a difference in the effect of GSSG treatment. The effectiveness of leafy vegetables with high zinc content in ameliorating zinc deficiency was evaluated by feeding lettuce with high zinc content to zinc-deficient mice. High zinc content lettuce significantly increased the zinc content in the liver, kidneys, gastrocnemius, and tibia of these mice. CONCLUSION: We established a cultivation method for lettuce and komatsuna with high zinc content without inhibiting growth by adjusting the zinc concentration in the hydroponic solution at an appropriate concentration for an appropriate period. The result of feeding test indicates that the intake of leafy vegetables with high zinc content can ameliorate zinc deficiency and might be useful in protection from several diseases associated with this deficiency. © 2020 Society of Chemical Industry.

Isotopic composition and concentration of total nitrogen and nitrate in xylem sap under near steady-state hydroponics.

After root uptake, nitrate is effluxed back to the medium, assimilated locally, or translocated to shoots. Rooted black cottonwood (Populus trichocarpa) scions were supplied with a NO3- -based (0.5 mM) nutrient medium of known isotopic composition (δ15 N), and xylem sap was collected by pressure bombing. To establish a sampling protocol, sap was collected from lower and upper stem sections at 0.1-0.2 MPa above the balancing pressure, and after increasing the pressure by a further 0.5 MPa. Xylem sap from upper stem sections was partially diluted at higher pressure. Further analysis was restricted to sap obtained from intact shoots at low pressure. Total-, NO3- -N and, by difference, organic-N concentrations ranged from 6.1-11.0, 1.2-2.4, and 4.6-9.4 mM, while discrimination relative to the nutrient medium was -6.3 to 0.5‰, -23.3 to -11.5‰ and - 1.3 to 4.9‰, respectively. There was diurnal variation in δ15 N of total- and organic-N, but not NO3- . The difference in δ15 N between xylem NO3- and organic-N suggests that discrimination by nitrate reductase is near 25.1 ± 1.6‰. When this value was used in an isotope mass balance model, the predicted xylem sap NO3- -N to total-N ratio closely matched direct measurement.

Metal Homeostasis and Gas Exchange Dynamics in Pisum sativum L. Exposed to Cerium Oxide Nanoparticles.

Cerium dioxide nanoparticles are pollutants of emerging concern. They are rarely immobilized in the environment. This study extends our work on Pisum sativum L. as a model plant, cultivated worldwide, and is well suited for investigating additive interactions induced by nanoceria. Hydroponic cultivation, which prompts accurate plant growth control and three levels of CeO2 supplementation, were applied, namely, 100, 200, and 500 mg (Ce)/L. Phytotoxicity was estimated by fresh weights and photosynthesis parameters. Additionally, Ce, Cu, Zn, Mn, Fe, Ca, and Mg contents were analyzed by high-resolution continuum source atomic absorption and inductively coupled plasma optical emission techniques. Analysis of variance has proved that CeO2 nanoparticles affected metals uptake. In the roots, it decreased for Cu, Zn, Mn, Fe, and Mg, while a reversed process was observed for Ca. The latter is absorbed more intensively, but translocation to above-ground parts is hampered. At the same time, nanoparticulate CeO2 reduced Cu, Zn, Mn, Fe, and Ca accumulation in pea shoots. The lowest Ce concentration boosted the photosynthesis rate, while the remaining treatments did not induce significant changes. Plant growth stimulation was observed only for the 100 mg/L. To our knowledge, this is the first study that demonstrates the effect of nanoceria on photosynthesis-related parameters in peas.

Rapid Hormetic Responses of Photosystem II Photochemistry of Clary Sage to Cadmium Exposure.

Five-day exposure of clary sage (Salvia sclarea L.) to 100 µM cadmium (Cd) in hydroponics was sufficient to increase Cd concentrations significantly in roots and aboveground parts and affect negatively whole plant levels of calcium (Ca) and magnesium (Mg), since Cd competes for Ca channels, while reduced Mg concentrations are associated with increased Cd tolerance. Total zinc (Zn), copper (Cu), and iron (Fe) uptake increased but their translocation to the aboveground parts decreased. Despite the substantial levels of Cd in leaves, without any observed defects on chloroplast ultrastructure, an enhanced photosystem II (PSII) efficiency was observed, with a higher fraction of absorbed light energy to be directed to photochemistry (ΦPSΙΙ). The concomitant increase in the photoprotective mechanism of non-photochemical quenching of photosynthesis (NPQ) resulted in an important decrease in the dissipated non-regulated energy (ΦNO), modifying the homeostasis of reactive oxygen species (ROS), through a decreased singlet oxygen (1O2) formation. A basal ROS level was detected in control plant leaves for optimal growth, while a low increased level of ROS under 5 days Cd exposure seemed to be beneficial for triggering defense responses, and a high level of ROS out of the boundaries (8 days Cd exposure), was harmful to plants. Thus, when clary sage was exposed to Cd for a short period, tolerance mechanisms were triggered. However, exposure to a combination of Cd and high light or to Cd alone (8 days) resulted in an inhibition of PSII functionality, indicating Cd toxicity. Thus, the rapid activation of PSII functionality at short time exposure and the inhibition at longer duration suggests a hormetic response and describes these effects in terms of "adaptive response" and "toxicity", respectively.

Hormesis in Plants: The Role of Oxidative Stress, Auxins and Photosynthesis in Corn Treated with Cd or Pb.

Hormesis, which describes the stimulatory effect of low doses of toxic substances on growth, is a well-known phenomenon in the plant and animal kingdoms. However, the mechanisms that are involved in this phenomenon are still poorly understood. We performed preliminary studies on corn coleoptile sections, which showed a positive correlation between the stimulation of growth by Cd or Pb and an increase in the auxin and H2O2 content in the coleoptile sections. Subsequently, we grew corn seedlings in hydroponic culture and tested a wide range of Cd or Pb concentrations in order to determine hormetic growth stimulation. In these seedlings the gas exchange and the chlorophyll a fluorescence, as well as the content of chlorophyll, flavonol, auxin and hydrogen peroxide, were measured. We found that during the hormetic stimulation of growth, the response of the photosynthetic apparatus to Cd and Pb differed significantly. While the application of Cd mostly caused a decrease in various photosynthetic parameters, the application of Pb stimulated some of them. Nevertheless, we discovered that the common features of the hormetic stimulation of shoot growth by heavy metals are an increase in the auxin and flavonol content and the maintenance of hydrogen peroxide at the same level as the control plants.

Enhancement of Macarpine Production in Eschscholzia Californica Suspension Cultures under Salicylic Acid Elicitation and Precursor Supplementation.

Macarpine is a minor benzophenanthridine alkaloid with interesting biological activities, which is produced in only a few species of the Papaveraceae family, including Eschscholzia californica. Our present study was focused on the enhancement of macarpine production in E. californica suspension cultures using three elicitation models: salicylic acid (SA) (4; 6; 8 mg/L) elicitation, and simultaneous or sequential combinations of SA and L-tyrosine (1 mmol/L). Sanguinarine production was assessed along with macarpine formation in elicited suspension cultures. Alkaloid production was evaluated after 24, 48 and 72 h of elicitation. Among the tested elicitation models, the SA (4 mg/L), supported by L-tyrosine, stimulated sanguinarine and macarpine production the most efficiently. While sequential treatment led to a peak accumulation of sanguinarine at 24 h and macarpine at 48 h, simultaneous treatment resulted in maximum sanguinarine accumulation at 48 h and macarpine at 72 h. The effect of SA elicitation and precursor supplementation was evaluated also based on the gene expression of 4'-OMT, CYP719A2, and CYP719A3. The gene expression of investigated enzymes was increased at all used elicitation models and their changes correlated with sanguinarine but not macarpine accumulation.