RESUMEN
RIG-I has a remarkable ability to specifically select viral 5'ppp dsRNAs for activation from a pool of cytosolic self-RNAs. The ATPase activity of RIG-I plays a role in RNA discrimination and activation, but the underlying mechanism was unclear. Using transient-state kinetics, we elucidated the ATPase-driven "kinetic proofreading" mechanism of RIG-I activation and RNA discrimination, akin to DNA polymerases, ribosomes, and T cell receptors. Even in the autoinhibited state of RIG-I, the C-terminal domain kinetically discriminates against self-RNAs by fast off rates. ATP binding facilitates dsRNA engagement but, interestingly, makes RIG-I promiscuous, explaining the constitutive signaling by Singleton-Merten syndrome-linked mutants that bind ATP without hydrolysis. ATP hydrolysis dissociates self-RNAs faster than 5'ppp dsRNA but, more importantly, drives RIG-I oligomerization through translocation, which we show to be regulated by helicase motif IVa. RIG-I translocates directionally from the dsRNA end into the stem region, and the 5'ppp end "throttles" translocation to provide a mechanism for threading and building a signaling-active oligomeric complex.
Asunto(s)
Adenosina Trifosfatasas/metabolismo , Proteína 58 DEAD Box/metabolismo , ARN/metabolismo , Adenosina Trifosfato/metabolismo , Enfermedades de la Aorta/metabolismo , Línea Celular , ARN Helicasas DEAD-box/metabolismo , Hipoplasia del Esmalte Dental/metabolismo , Femenino , Células HEK293 , Humanos , Hidrólisis , Cinética , Metacarpo/anomalías , Metacarpo/metabolismo , Enfermedades Musculares/metabolismo , Odontodisplasia/metabolismo , Osteoporosis/metabolismo , Unión Proteica/fisiología , ARN Bicatenario/metabolismo , Receptores de Antígenos de Linfocitos T/metabolismo , Receptores Inmunológicos , Ribosomas/metabolismo , Transducción de Señal/fisiología , Calcificación Vascular/metabolismoRESUMEN
Singleton-Merten syndrome (SMS) is a rare immunogenetic disorder affecting multiple systems, characterized by dental dysplasia, aortic calcification, glaucoma, skeletal abnormalities, and psoriasis. Glaucoma, a key feature of both classical and atypical SMS, remains poorly understood in terms of its molecular mechanism caused by DDX58 mutation. This study presented a novel DDX58 variant (c.1649A>C [p.Asp550Ala]) in a family with childhood glaucoma. Functional analysis showed that DDX58 variant caused an increase in IFN-stimulated gene expression and high IFN-ß-based type-I IFN. As the trabecular meshwork (TM) is responsible for controlling intraocular pressure (IOP), we examine the effect of IFN-ß on TM cells. Our study is the first to demonstrate that IFN-ß significantly reduced TM cell viability and function by activating autophagy. In addition, anterior chamber injection of IFN-ß remarkably increased IOP level in mice, which can be attenuated by treatments with autophagy inhibitor chloroquine. To uncover the specific mechanism underlying IFN-ß-induced autophagy in TM cells, we performed microarray analysis in IFN-ß-treated and DDX58 p.Asp550Ala TM cells. It showed that RSAD2 is necessary for IFN-ß-induced autophagy. Knockdown of RSAD2 by siRNA significantly decreased autophagy flux induced by IFN-ß. Our findings suggest that DDX58 mutation leads to the overproduction of IFN-ß, which elevates IOP by modulating autophagy through RSAD2 in TM cells.
Asunto(s)
Autofagia , Proteína 58 DEAD Box , Glaucoma , Presión Intraocular , Malla Trabecular , Animales , Femenino , Humanos , Masculino , Ratones , Enfermedades de la Aorta , Autofagia/efectos de los fármacos , Proteína 58 DEAD Box/metabolismo , Proteína 58 DEAD Box/genética , Hipoplasia del Esmalte Dental , Glaucoma/patología , Glaucoma/metabolismo , Glaucoma/genética , Pérdida Auditiva Sensorineural/genética , Pérdida Auditiva Sensorineural/patología , Pérdida Auditiva Sensorineural/metabolismo , Interferón beta/metabolismo , Presión Intraocular/genética , Metacarpo/anomalías , Ratones Endogámicos C57BL , Enfermedades Musculares , Mutación , Odontodisplasia , Atrofia Óptica/genética , Atrofia Óptica/metabolismo , Atrofia Óptica/patología , Osteoporosis , Linaje , Receptores Inmunológicos , Malla Trabecular/metabolismo , Malla Trabecular/efectos de los fármacos , Calcificación VascularRESUMEN
DDX58 encodes RIG-I, a cytosolic RNA sensor that ensures immune surveillance of nonself RNAs. Individuals with RIG-IE510V and RIG-IQ517H mutations have increased susceptibility to Singleton-Merten syndrome (SMS) defects, resulting in tissue-specific (mild) and classic (severe) phenotypes. The coupling between RNA recognition and conformational changes is central to RIG-I RNA proofreading, but the molecular determinants leading to dissociated disease phenotypes remain unknown. Herein, we employed hydrogen/deuterium exchange mass spectrometry (HDX-MS) and single molecule magnetic tweezers (MT) to precisely examine how subtle conformational changes in the helicase insertion domain (HEL2i) promote impaired ATPase and erroneous RNA proofreading activities. We showed that the mutations cause a loosened latch-gate engagement in apo RIG-I, which in turn gradually dampens its self RNA (Cap2 moiety:m7G cap and N1-2-2'-O-methylation RNA) proofreading ability, leading to increased immunopathy. These results reveal HEL2i as a unique checkpoint directing two specialized functions, i.e. stabilizing the CARD2-HEL2i interface and gating the helicase from incoming self RNAs; thus, these findings add new insights into the role of HEL2i in the control of antiviral innate immunity and autoimmunity diseases.
Asunto(s)
Enfermedades Autoinmunes , Odontodisplasia , Enfermedades Autoinmunes/genética , Proteína 58 DEAD Box/química , Proteína 58 DEAD Box/genética , ARN Helicasas DEAD-box/química , ARN Helicasas DEAD-box/genética , Humanos , Inmunidad Innata/genética , Metacarpo , ARN/químicaRESUMEN
Segmental odontomaxillary dysplasia (SOD) is a rare and unusual nonhereditary developmental disorder that affects one side of the maxilla, impacting the hard tissue, soft tissue, and dentition in the affected area. It most frequently presents with enlargement of the gingival and osseous tissue of the affected side and hypodontia of the involved quadrant. Cutaneous irregularities of the impacted area are also common. We report a case of SOD arising in the right maxilla of a three-year-old female. Our report and review of the literature highlight the clinical, radiographic, and histopathologic characteristics of SOD, as well as the management of patients and the proposed etiologies of its pathogenesis.
Asunto(s)
Odontodisplasia , Humanos , Femenino , Preescolar , Odontodisplasia/patología , Odontodisplasia/diagnóstico por imagen , Odontodisplasia/diagnóstico , Maxilar/anomalíasRESUMEN
BACKGROUND: Singleton-Merten syndrome (SGMRT) is a rare immunogenetic disorder that variably features juvenile open-angle glaucoma (JOAG), psoriasiform skin rash, aortic calcifications and skeletal and dental dysplasia. Few families have been described and the genotypic and phenotypic spectrum is poorly defined, with variants in DDX58 (DExD/H-box helicase 58) being one of two identified causes, classified as SGMRT2. METHODS: Families underwent deep systemic phenotyping and exome sequencing. Functional characterisation with in vitro luciferase assays and in vivo interferon signature using bulk and single cell RNA sequencing was performed. RESULTS: We have identified a novel DDX58 variant c.1529A>T p.(Glu510Val) that segregates with disease in two families with SGMRT2. Patients in these families have widely variable phenotypic features and different ethnic background, with some being severely affected by systemic features and others solely with glaucoma. JOAG was present in all individuals affected with the syndrome. Furthermore, detailed evaluation of skin rash in one patient revealed sparse inflammatory infiltrates in a unique distribution. Functional analysis showed that the DDX58 variant is a dominant gain-of-function activator of interferon pathways in the absence of exogenous RNA ligands. Single cell RNA sequencing of patient lesional skin revealed a cellular activation of interferon-stimulated gene expression in keratinocytes and fibroblasts but not in neighbouring healthy skin. CONCLUSIONS: These results expand the genotypic spectrum of DDX58-associated disease, provide the first detailed description of ocular and dermatological phenotypes, expand our understanding of the molecular pathogenesis of this condition and provide a platform for testing response to therapy.
Asunto(s)
Exantema , Glaucoma de Ángulo Abierto , Odontodisplasia , Proteína 58 DEAD Box/genética , Exantema/patología , Glaucoma de Ángulo Abierto/patología , Humanos , Interferones/genética , Metacarpo/patología , Odontodisplasia/genética , Odontodisplasia/patología , Receptores InmunológicosRESUMEN
OBJECTIVE: This review aimed to summarize recent progress on syndromic dentin defects, promoting a better understanding of systemic diseases with dentin malformations, the molecules involved, and related mechanisms. SUBJECTS AND METHODS: References on genetic diseases with dentin malformations were obtained from various sources, including PubMed, OMIM, NCBI, and other websites. The clinical phenotypes and genetic backgrounds of these diseases were then summarized, analyzed, and compared. RESULTS: Over 10 systemic diseases, including osteogenesis imperfecta, hypophosphatemic rickets, vitamin D-dependent rickets, familial tumoral calcinosis, Ehlers-Danlos syndrome, Schimke immuno-osseous dysplasia, hypophosphatasia, Elsahy-Waters syndrome, Singleton-Merten syndrome, odontochondrodysplasia, and microcephalic osteodysplastic primordial dwarfism type II were examined. Most of these are bone disorders, and their pathogenic genes may regulate both dentin and bone development, involving extracellular matrix, cell differentiation, and metabolism of calcium, phosphorus, and vitamin D. The phenotypes of these syndromic dentin defects various with the involved genes, part of them are similar to dentinogenesis imperfecta or dentin dysplasia, while others only present one or two types of dentin abnormalities such as discoloration, irregular enlarged or obliterated pulp and canal, or root malformation. CONCLUSION: Some specific dentin defects associated with systemic diseases may serve as important phenotypes for dentists to diagnose. Furthermore, mechanistic studies on syndromic dentin defects may provide valuable insights into isolated dentin defects and general dentin development or mineralization.
Asunto(s)
Dentinogénesis Imperfecta , Odontodisplasia , Osteogénesis Imperfecta , Humanos , Dentinogénesis Imperfecta/genética , Odontodisplasia/patología , Osteogénesis Imperfecta/patología , Dentina , Vitamina DRESUMEN
Regional odontodysplasia (RO) is a rare non-hereditary dental anomaly associated with dysplasia. Its etiology remains unclear but is known to affect both the mesodermal and ectodermal dental components, as well as deciduous and permanent dentitions. Its young age of onset and complexity has great physical and psychological impact on the affected patients. However, the clinical management of RO remains unified without standardized treatment guidelines. Thus, this study aimed to report an RO case, the first from Jiangxi Province, China, and discuss its clinical diagnosis and treatment to provide a reference to treat similar cases more effectively in the future.
Asunto(s)
Odontodisplasia , Humanos , Odontodisplasia/diagnóstico , Odontodisplasia/terapia , Odontodisplasia/complicaciones , Dentición PermanenteRESUMEN
Regional odontodysplasia (RO) is a rare developmental abnormality of epithelial and mesenchymal dental tissues. Due to its poorly understood etiology, assessing and discussing related clinical cases of this dental anomaly is crucial to guide professionals in improving its treatment and outcomes. This article aimed to report the case of a 9-year-old male patient who presented to our department with the main complaint of absent eruption of permanent left mandibular quadrant teeth. This is the first case reported in China from a patient with multiple cutaneous nevi on the face and neck, and based on the retrieved clinical and radiographic features, we described and discussed the treatment and etiology of RO.
Asunto(s)
Odontodisplasia , Masculino , Humanos , Niño , Odontodisplasia/diagnóstico por imagen , Cuello , Mandíbula , Erupción DentalRESUMEN
Mutations in DDX58 (DExD/H-box helicase 58), which encodes the cytoplasmic RNA sensor retinoic acid-inducible gene I (RIG-I), were recently identified in the rare autoimmune disease Singleton-Merten syndrome (SMS). We report the spontaneous development of psoriasis-like skin lesions as an SMS-like symptom in transgenic mice harboring one of the RIG-I SMS variants, E373A. Histological analysis revealed typical characteristics of psoriasis, including the abnormal proliferation and differentiation of keratinocytes leading to epidermal hyperplasia, and infiltrates consisting of neutrophils, dendritic cells and T cells. Levels of the IL-23/IL-17 immune axis cytokines were high in the skin lesions. Rag2-/- transgenic mice showed partial amelioration of the phenotype, with down-regulation of inflammatory cytokines, including IL-17A, suggesting the importance of lymphocytes for the pathogenesis similar to that of human psoriasis. Of note, IL-17A deficiency abolished the skin phenotype, and treatment using the JAK inhibitor tofacitinib not only prevented onset, but also improved the skin manifestations even after onset. Our study provides further evidence for the involvement of RIG-I activation in the onset and progression of psoriasis via type I interferon signaling and the IL-23/IL-17 axis.
Asunto(s)
Proteína 58 DEAD Box/genética , Proteína 58 DEAD Box/metabolismo , Interferón Tipo I/inmunología , Interleucina-17/metabolismo , Subunidad p19 de la Interleucina-23/metabolismo , Psoriasis/patología , Animales , Enfermedades de la Aorta/genética , Proteínas de Unión al ADN/genética , Células Dendríticas/inmunología , Hipoplasia del Esmalte Dental/genética , Epidermis/patología , Hiperplasia/genética , Hiperplasia/patología , Inhibidores de las Cinasas Janus/farmacología , Quinasas Janus/antagonistas & inhibidores , Queratinocitos/citología , Queratinocitos/patología , Metacarpo/anomalías , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Enfermedades Musculares/genética , Neutrófilos/inmunología , Odontodisplasia/genética , Osteoporosis/genética , Piperidinas/farmacología , Psoriasis/genética , Pirimidinas/farmacología , Linfocitos T/inmunología , Calcificación Vascular/genéticaRESUMEN
Pathogenic-activating variants of interferon induced with Helicase C domain 1 (IFIH1) cause Singleton-Merten (S-M) syndrome, which accompanies acro-osteolysis, loss of permanent teeth, and aortic calcification, as well as causing Aicardi-Goutières (A-G) syndrome, which shows progressive encephalopathy, spastic paraplegia, and calcification of basal ganglia. Recently, patients with overlapping syndromes presenting with features of S-M syndrome and A-G syndrome were reported. However, progression of clinical features of this condition has not been fully understood. We report a Japanese boy with a novel pathogenic IFIH1 variant who presented with clinical features of S-M syndrome and A-G syndrome.
Asunto(s)
Enfermedades Autoinmunes del Sistema Nervioso , Interferones , Enfermedades de la Aorta , Enfermedades Autoinmunes del Sistema Nervioso/diagnóstico , Enfermedades Autoinmunes del Sistema Nervioso/genética , Enfermedades Autoinmunes del Sistema Nervioso/patología , Hipoplasia del Esmalte Dental , Humanos , Helicasa Inducida por Interferón IFIH1/genética , Japón , Masculino , Metacarpo/anomalías , Enfermedades Musculares , Malformaciones del Sistema Nervioso , Odontodisplasia , Osteoporosis , Calcificación VascularRESUMEN
Arterial calcification is a common phenomenon in the elderly, in patients with atherosclerosis or renal failure and in diabetes. However, when present in very young individuals, it is likely to be associated with an underlying hereditary disorder of arterial calcification. Here, we present an overview of the few monogenic disorders presenting with early-onset cardiovascular calcification. These disorders can be classified according to the function of the respective disease gene into (1) disorders caused by an altered purine and phosphate/pyrophosphate metabolism, (2) interferonopathies, and (3) Gaucher disease. The finding of arterial calcification in early life should alert the clinician and prompt further genetic work-up to define the underlying genetic defect, to establish the correct diagnosis, and to enable appropriate therapy.
Asunto(s)
Enfermedades de la Aorta/genética , Arterias/metabolismo , Hipoplasia del Esmalte Dental/genética , Herencia , Errores Innatos del Metabolismo/genética , Metacarpo/anomalías , Enfermedades Musculares/genética , Odontodisplasia/genética , Osteogénesis/genética , Osteoporosis/genética , Calcificación Vascular/genética , Animales , Enfermedades de la Aorta/complicaciones , Enfermedades de la Aorta/metabolismo , Arterias/patología , Hipoplasia del Esmalte Dental/complicaciones , Hipoplasia del Esmalte Dental/metabolismo , Enfermedad de Gaucher/complicaciones , Enfermedad de Gaucher/genética , Enfermedad de Gaucher/metabolismo , Predisposición Genética a la Enfermedad , Humanos , Errores Innatos del Metabolismo/complicaciones , Errores Innatos del Metabolismo/metabolismo , Metacarpo/metabolismo , Enfermedades Musculares/complicaciones , Enfermedades Musculares/metabolismo , Odontodisplasia/complicaciones , Odontodisplasia/metabolismo , Osteoporosis/complicaciones , Osteoporosis/metabolismo , Fenotipo , Medición de Riesgo , Factores de Riesgo , Calcificación Vascular/complicaciones , Calcificación Vascular/metabolismo , Calcificación Vascular/patologíaRESUMEN
Singleton-Merten syndrome (SMS) is a type I interferonopathy. In this report, we disclose the first-to the best of our knowledge-direct association of SMS with femoral head necrosis (FHN). The following case report presents the condition of a 38-year-old male suffering from SMS with FHN, characterized by acute symptoms and rapid disease progression. As per the recommendations of the Association Research Circulation Osseous (ARCO) and the S3-guidelines, we successfully treated the FHN with core decompression. Our histological results correlate with the changes described in medical literature in patients with SMS and MDA5-knockout in vivo experiments such as osteopenia, widened medullary cavity, and thin cortical bone. Moreover, the conducted immunohistochemistry shows strong CD56 positivity of the osteoblasts and osteocytes, as well as significant CD68 and CD163 positivity of the middle-sized osteoclasts. Collectively, these findings suggest an underlying syndrome in the FHN. A six-month post-operative follow-up revealed complete recovery with the absence of the initial symptoms and ability to resume normal daily activities. Taken together, our findings suggest that SMS is an additional cause of FHN in young adults. Early detection and adequate treatment using well-established joint-preserving techniques demonstrate a favorable improvement of the patient's clinical condition.
Asunto(s)
Enfermedades de la Aorta/genética , Hipoplasia del Esmalte Dental/genética , Necrosis de la Cabeza Femoral/genética , Interferones/genética , Metacarpo/anomalías , Enfermedades Musculares/genética , Odontodisplasia/genética , Osteoporosis/genética , Anomalías Cutáneas/genética , Calcificación Vascular/genética , Adulto , Antígenos CD/genética , Antígenos de Diferenciación Mielomonocítica/genética , Enfermedades de la Aorta/complicaciones , Enfermedades de la Aorta/patología , Antígeno CD56/genética , Hipoplasia del Esmalte Dental/complicaciones , Hipoplasia del Esmalte Dental/patología , Cabeza Femoral/patología , Necrosis de la Cabeza Femoral/complicaciones , Necrosis de la Cabeza Femoral/patología , Humanos , Masculino , Metacarpo/patología , Enfermedades Musculares/complicaciones , Enfermedades Musculares/patología , Odontodisplasia/complicaciones , Odontodisplasia/patología , Osteoporosis/complicaciones , Osteoporosis/patología , Receptores de Superficie Celular/genética , Anomalías Cutáneas/patología , Resultado del Tratamiento , Calcificación Vascular/complicaciones , Calcificación Vascular/patologíaRESUMEN
Singleton-Merten syndrome (SMS) is a type I interferonopathy characterized by dental dysplasia, aortic calcification, skeletal abnormalities, glaucoma, and psoriasis. A missense mutation in IFIH1 encoding a cytoplasmic viral RNA sensor MDA5 has recently been identified in the SMS patients as well as in patients with a monogenic form of lupus. We previously reported that Ifih1gs/+ mice express a constitutively active MDA5 and spontaneously develop lupus-like nephritis. In this study, we demonstrate that the Ifih1gs/+ mice also exhibit SMS-like bone abnormalities, including decreased bone mineral density and thin cortical bone. Histological analysis revealed a low number of osteoclasts, low bone formation rate, and abnormal development of growth plate cartilages in Ifih1gs/+ mice. These abnormalities were not observed in Ifih1gs/+ ã»Mavs-/- and Ifih1gs/+ ã»Ifnar1-/- mice, indicating the critical role of type I IFNs induced by MDA5/MAVS-dependent signaling in the bone pathogenesis of Ifih1gs/+ mice, affecting bone turnover. Taken together, our findings suggest the inhibition of type I IFN signaling as a possible effective therapeutic strategy for bone disorders in SMS patients.
Asunto(s)
Enfermedades de la Aorta/metabolismo , Enfermedades Óseas/metabolismo , Huesos/anomalías , Huesos/metabolismo , Hipoplasia del Esmalte Dental/metabolismo , Helicasa Inducida por Interferón IFIH1/metabolismo , Metacarpo/anomalías , Enfermedades Musculares/metabolismo , Odontodisplasia/metabolismo , Osteoporosis/metabolismo , Calcificación Vascular/metabolismo , Animales , Enfermedades de la Aorta/genética , Enfermedades Óseas/genética , Cartílago/metabolismo , Hipoplasia del Esmalte Dental/genética , Placa de Crecimiento/metabolismo , Masculino , Metacarpo/metabolismo , Ratones , Ratones Endogámicos C57BL , Enfermedades Musculares/genética , Mutación Missense/genética , Odontodisplasia/genética , Osteoporosis/genética , Calcificación Vascular/genéticaRESUMEN
PURPOSE: Singleton-Merten syndrome manifests as dental dysplasia, glaucoma, psoriasis, aortic calcification, and skeletal abnormalities including tendon rupture and arthropathy. Pathogenic variants in IFIH1 have previously been associated with the classic Singleton-Merten syndrome, while variants in DDX58 has been described in association with a milder phenotype, which is suggested to have a better prognosis. We studied a family with severe, "classic" Singleton-Merten syndrome. METHODS: We undertook clinical phenotyping, next-generation sequencing, and functional studies of type I interferon production in patient whole blood and assessed the type I interferon promoter activity in HEK293 cells transfected with wild-type or mutant DDX58 stimulated with Poly I:C. RESULTS: We demonstrate a DDX58 autosomal dominant gain-of-function mutation, with constitutive upregulation of type I interferon. CONCLUSIONS: DDX58 mutations may be associated with the classic features of Singleton-Merten syndrome including dental dysplasia, tendon rupture, and severe cardiac sequela.
Asunto(s)
Enfermedades de la Aorta/genética , Proteína 58 DEAD Box/genética , Hipoplasia del Esmalte Dental/genética , Metacarpo/anomalías , Enfermedades Musculares/genética , Odontodisplasia/genética , Osteoporosis/genética , Calcificación Vascular/genética , Adulto , Línea Celular , Femenino , Mutación con Ganancia de Función/genética , Células HEK293 , Humanos , Interferón Tipo I/genética , Masculino , Persona de Mediana Edad , Fenotipo , Regiones Promotoras Genéticas/genética , Receptores InmunológicosRESUMEN
Janus kinase (JAK)/signal transducers and activators of transcription (STATs) are a group of molecules associated with one of the major pathways through which many cytokines exert and integrate their function, and as such they are increasingly recognized as playing critical role in the pathogenesis subserving various immune-mediated diseases, including RA, PsA, SpAs, IBD, skin disorders (e.g. alopecia areata, atopic dermatitis), single-gene disorders like interferonopathies, and others. JAKs are the key initiating players of the JAK/STAT pathway. Upon binding of their respective effector molecules (cytokines, IFNs, growth factors and others) to type I and type II receptors, JAKs are activated, and through phosphorylation of themselves and of other molecules (including STATs), they mediate signal transduction to the nucleus. A class of drugs-called JAK inhibitors or JAKinibs-that block one or more JAKs has been developed in the last decade, and now numbers >20 members. Although, so far, JAK inhibitors have been marketed only for RA and PsA, these drugs have been tested in phase 2 and phase 3 clinical trials for other inflammatory conditions and beyond. In this review, we summarize the clinical data, including efficacy and safety, available for JAK inhibitors used in some immune-mediated conditions other than RA.
Asunto(s)
Artritis Psoriásica/tratamiento farmacológico , Enfermedades Inflamatorias del Intestino/tratamiento farmacológico , Inhibidores de las Cinasas Janus/uso terapéutico , Espondiloartropatías/tratamiento farmacológico , Alopecia Areata/tratamiento farmacológico , Enfermedades de la Aorta/tratamiento farmacológico , Artritis Reumatoide/tratamiento farmacológico , Enfermedades Autoinmunes/tratamiento farmacológico , Enfermedades Autoinmunes del Sistema Nervioso/tratamiento farmacológico , Eritema Pernio/tratamiento farmacológico , Citocinas , Hipoplasia del Esmalte Dental/tratamiento farmacológico , Dermatitis Atópica/tratamiento farmacológico , Arteritis de Células Gigantes/tratamiento farmacológico , Humanos , Síndromes de Inmunodeficiencia , Lupus Eritematoso Cutáneo/tratamiento farmacológico , Metacarpo/anomalías , Enfermedades Musculares/tratamiento farmacológico , Malformaciones del Sistema Nervioso/tratamiento farmacológico , Odontodisplasia/tratamiento farmacológico , Osteoporosis/tratamiento farmacológico , Psoriasis/tratamiento farmacológico , Uveítis/tratamiento farmacológico , Calcificación Vascular/tratamiento farmacológicoRESUMEN
Odonto-onycho-dermal dysplasia (OODD) is a rare autosomal recessive syndrome characterized by multiple ectodermal abnormalities. Mutations of the wingless-type MMTV integration site family member 10A (WNT10A) gene have been associated with OODD. To date, only 11 OODD-associated WNT10A mutations have been reported. In this report, we Characterized the clinical manifestations with focusing on dental phenotypes in four unrelated OODD patients. By Sanger sequencing, we identified five novel mutations in the WNT10A gene, including two homozygous nonsense mutations c.1176C>A (p.Cys392*) and c.742C>T (p.Arg248*), one homozygous frame-shift mutation c.898-899delAT (p.Ile300Profs*126), and a compound heterozygous mutation c.826T>A (p.Cys276Ser) and c.949delG (p.Ala317Hisfs*121). Our findings confirmed that bi-allelic mutations of WNT10A were responsible for OODD and greatly expanded the mutation spectrum of OODD. For the first time, we demonstrated that bi-allelic WNT10A mutations could lead to anodontia of permanent teeth, which enhanced the phenotypic spectrum of WNT10A mutations. Interestingly, we found that bi-allelic mutations in the WNT10A gene preferentially affect the permanent dentition rather the primary dentition, suggesting that the molecular mechanisms regulated by WNT10A in the development of permanent teeth and deciduous teeth might be different.
Asunto(s)
Anodoncia/genética , Displasia Ectodérmica/genética , Predisposición Genética a la Enfermedad , Odontodisplasia/genética , Proteínas Wnt/genética , Anomalías Múltiples/diagnóstico por imagen , Anomalías Múltiples/genética , Anomalías Múltiples/fisiopatología , Adolescente , Alelos , Anodoncia/diagnóstico por imagen , Niño , Codón sin Sentido/genética , Displasia Ectodérmica/diagnóstico por imagen , Displasia Ectodérmica/fisiopatología , Femenino , Mutación del Sistema de Lectura/genética , Homocigoto , Humanos , Masculino , Odontodisplasia/diagnóstico por imagen , Odontodisplasia/fisiopatología , FenotipoRESUMEN
OBJECTIVE: Tooth agenesis is one of the most common craniofacial developmental anomalies. In hypodontia, one to five teeth are missing, whereas oligodontia refers to the absence of at least six teeth, excluding the third molars. Mutations in several genes including MSX1, PAX9, AXIN2, and WNT10A have been shown to cause non-syndromic tooth agenesis. Regional odontodysplasia (RO), also known as "ghost teeth," is a rare developmental anomaly of tooth formation affecting both dentitions. Some possible causes of RO have been suggested, yet the etiology remains unknown. Because the phenotypes of both oligodontia and RO co-occur in one Finnish family, the aim here was to investigate the genetic etiology of the two conditions. MATERIALS AND METHODS: A mutation screening of the genes MSX1, PAX9, AXIN2, and WNT10A was performed for the family members of a RO patient and family history of oligodontia. RESULTS: An initiation codon mutation of the PAX9 gene was found in the proband and segregating with oligodontia in the family. CONCLUSIONS: The etiology of regional odontodysplasia (RO) may be genetic and the same genes can be involved both in RO and tooth agenesis. CLINICAL RELEVANCE: Our results give new insights into the etiology of regional odontodysplasia, yet further results are needed.
Asunto(s)
Anodoncia , Odontodisplasia , Factor de Transcripción PAX9 , Anodoncia/genética , Codón Iniciador , Humanos , Factor de Transcripción MSX1 , Mutación , Odontodisplasia/genética , Factor de Transcripción PAX9/genética , LinajeRESUMEN
Singleton-Merten syndrome (SMS) is an autosomal-dominant multi-system disorder characterized by dental dysplasia, aortic calcification, skeletal abnormalities, glaucoma, psoriasis, and other conditions. Despite an apparent autosomal-dominant pattern of inheritance, the genetic background of SMS and information about its phenotypic heterogeneity remain unknown. Recently, we found a family affected by glaucoma, aortic calcification, and skeletal abnormalities. Unlike subjects with classic SMS, affected individuals showed normal dentition, suggesting atypical SMS. To identify genetic causes of the disease, we performed exome sequencing in this family and identified a variant (c.1118A>C [p.Glu373Ala]) of DDX58, whose protein product is also known as RIG-I. Further analysis of DDX58 in 100 individuals with congenital glaucoma identified another variant (c.803G>T [p.Cys268Phe]) in a family who harbored neither dental anomalies nor aortic calcification but who suffered from glaucoma and skeletal abnormalities. Cys268 and Glu373 residues of DDX58 belong to ATP-binding motifs I and II, respectively, and these residues are predicted to be located closer to the ADP and RNA molecules than other nonpathogenic missense variants by protein structure analysis. Functional assays revealed that DDX58 alterations confer constitutive activation and thus lead to increased interferon (IFN) activity and IFN-stimulated gene expression. In addition, when we transduced primary human trabecular meshwork cells with c.803G>T (p.Cys268Phe) and c.1118A>C (p.Glu373Ala) mutants, cytopathic effects and a significant decrease in cell number were observed. Taken together, our results demonstrate that DDX58 mutations cause atypical SMS manifesting with variable expression of glaucoma, aortic calcification, and skeletal abnormalities without dental anomalies.
Asunto(s)
Enfermedades de la Aorta/genética , ARN Helicasas DEAD-box/genética , Hipoplasia del Esmalte Dental/genética , Glaucoma/genética , Metacarpo/anomalías , Modelos Moleculares , Enfermedades Musculares/genética , Odontodisplasia/genética , Osteoporosis/genética , Calcificación Vascular/genética , Adulto , Enfermedades de la Aorta/patología , Secuencia de Bases , Células Cultivadas , Preescolar , Proteína 58 DEAD Box , ARN Helicasas DEAD-box/química , Hipoplasia del Esmalte Dental/patología , Exoma/genética , Femenino , Genes Dominantes/genética , Humanos , Masculino , Metacarpo/patología , Datos de Secuencia Molecular , Enfermedades Musculares/patología , Anomalías Musculoesqueléticas/diagnóstico por imagen , Anomalías Musculoesqueléticas/genética , Mutación Missense/genética , Odontodisplasia/diagnóstico por imagen , Odontodisplasia/patología , Osteoporosis/patología , Linaje , Polimorfismo de Nucleótido Simple/genética , Radiografía , Receptores Inmunológicos , Análisis de Secuencia de ADN , Calcificación Vascular/patologíaRESUMEN
Singleton-Merten syndrome (SMS) is an infrequently described autosomal-dominant disorder characterized by early and extreme aortic and valvular calcification, dental anomalies (early-onset periodontitis and root resorption), osteopenia, and acro-osteolysis. To determine the molecular etiology of this disease, we performed whole-exome sequencing and targeted Sanger sequencing. We identified a common missense mutation, c.2465G>A (p.Arg822Gln), in interferon induced with helicase C domain 1 (IFIH1, encoding melanoma differentiation-associated protein 5 [MDA5]) in four SMS subjects from two families and a simplex case. IFIH1 has been linked to a number of autoimmune disorders, including Aicardi-Goutières syndrome. Immunohistochemistry demonstrated the localization of MDA5 in all affected target tissues. In vitro functional analysis revealed that the IFIH1 c.2465G>A mutation enhanced MDA5 function in interferon beta induction. Interferon signature genes were upregulated in SMS individuals' blood and dental cells. Our data identify a gain-of-function IFIH1 mutation as causing SMS and leading to early arterial calcification and dental inflammation and resorption.
Asunto(s)
Enfermedades de la Aorta/genética , ARN Helicasas DEAD-box/genética , Hipoplasia del Esmalte Dental/genética , Metacarpo/anomalías , Modelos Moleculares , Enfermedades Musculares/genética , Odontodisplasia/genética , Osteoporosis/genética , Fenotipo , Calcificación Vascular/genética , Secuencia de Aminoácidos , Arterias/patología , Secuencia de Bases , Calcinosis/genética , Calcinosis/patología , ARN Helicasas DEAD-box/química , ARN Helicasas DEAD-box/metabolismo , Exoma/genética , Genes Dominantes/genética , Humanos , Inmunohistoquímica , Helicasa Inducida por Interferón IFIH1 , Interferón beta/metabolismo , Datos de Secuencia Molecular , Mutación Missense/genética , Linaje , Análisis de Secuencia de ADN , Anomalías Dentarias/genética , Anomalías Dentarias/patologíaRESUMEN
Molar apical elongation (MAE) was the leading cause for euthanasia or death in a captive breeding colony of endangered Amargosa voles ( Microtus californicus scirpensis). Clinical signs included ocular discharge, abnormal mastication, dyspnea, abnormal mentation, weight loss, and death. Although the severity varied, all molars in all quadrants were affected. When severe, the overgrown molar reserve crown and apex protruded into the nasal meatuses, the orbit, the calvarial vault and through the ventral margin of the mandible. Overall prevalence in the colony was 63% (92/146 voles) and increased to 77% in aged voles (>1 year). Mean age of onset was 5.3 months (1.7-11.2 months). Progression to extreme severity occurred over 1 to 3 months. Mean survival was 10.9 months (7.1-21.7 months). Histologically, the lesion was characterized by odontogenic hyperplasia and dysplasia. MAE was also documented in museum specimens of 2 other M. californicus subspecies ( M. californicus californicus, M. californicus vallicola) and 3 other Microtus species ( M. montanus, M. pennsylvanicus, M. socialis). In the M. californicus californicus collection, overall prevalence was 35.1% (129/368 skulls) and increased to 77.3% in aged voles (>1 year). A probable genetic influence was identified in the museum collection of M. californicus californicus. The etiopathogenesis of MAE is likely multifactorial, due to (1) inherent continuous odontogenic proliferation, (2) inadequate occlusal attrition, and (3) possible heritable disease susceptibility. In captivity, dietary or other management of occlusal attrition to prevent or delay MAE is a fundamental concern.