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Arch Virol ; 157(4): 773-6, 2012 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-22227800

RESUMEN

In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-throughput sequencing. The full genome of a new potyvirus could be assembled from the resulting siRNA sequences, and it was sufficiently different from other sequences to be considered a member of a new species, which we have designated Yam bean mosaic virus (YBMV). Sequence similarity suggests that YBMV has also been detected in yam beans in Indonesia.


Asunto(s)
Genoma Viral , Pachyrhizus/virología , Enfermedades de las Plantas/virología , Potyvirus/genética , Potyvirus/aislamiento & purificación , ARN Viral/genética , Análisis por Conglomerados , Datos de Secuencia Molecular , Perú , Filogenia , ARN Interferente Pequeño/genética , Análisis de Secuencia de ADN , Homología de Secuencia
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