ABSTRACT
Purpose:
To evaluate the effects of
sucralfate enemas in
tissue contents of
E-cadherin and ?-
catenin in an experimental diversion
colitis.
Methods:
Thirty-six
male Wistar rats were submitted to a proximal
colostomy and a distal mucous
fistula. They were allocated into three groups first group received daily saline
enemas (2 mL/day) and the two other groups daily
enemas with
sucralfate at
dosage of 1 or 2 g/kg/day, respectively. Six
animals of each group were euthanized after two weeks and six
animals after four weeks. The
inflammation of the excluded
mucosa was evaluated by histological
analysis. The
oxidative damage was quantified by measurement of
malondialdehyde tissue levels. The expression of
E-cadherin and ?-
catenin was identified by
immunohistochemistry, and its contents were quantified by
computer-assisted image analysis.
Results:
Sucralfate enemas reduced
inflammation in
animals subjected to
treatment with 2 g/kg/day by four weeks, and the levels of
oxidative damage in
mucosa without fecal
stream irrespective of concentration and
time of intervention.
E-cadherin and ?-
catenin content increased in segments without fecal
stream in those
animals subjected to
treatment with
sucralfate.
Conclusions:
Sucralfate reduces the
inflammation and
oxidative stress and increases the
tissue content of
E-cadherin and ?-
catenin in colonic
mucosa devoid to the fecal
stream.