Cyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic rats
Lemos Neto, Sylvio Valença de; Vianna, Isabela Galvão; Castiglia, Yara Marcondes Machado; Golim, Marjorie de Assis; Souza, Aparecida Vitória Gonçalves de; Carvalho, Lídia Raquel de; Deffune, Elenice; Nascimento Junior, Paulo do; Módolo, Norma Sueli Pinheiro; Vianna, Pedro Thadeu Galvão.
Acta cir. bras;
32(3): 203-210, Mar. 2017. tab
Artículo
en Inglés
| LILACS | ID: biblio-837689
Abstract
Purpose:
To investigate the effects of cyclosporine A on renal ischemia-reperfusion injury during transient hyperglycemia in rats.Methods:
In a model of ischemia-reperfusion-induced renal injury and transiently induced hyperglycemia by intraperitoneal injection of glucose, 2.5 g.kg-1, Wistar rats were anesthetized with either isoflurane or propofol and received intravenous cyclosporine A, 5 mg.kg-1, five minutes before reperfusion. Comparison groups were isoflurane and propofol sham groups and isoflurane and propofol ischemia-reperfusion-induced renal injury. Renal tubular cell viability was quantitatively assessed by flow cytometry after cell culture and classified as early apoptosis, necrotic cells, and intact cells.Results:
Early apoptosis was significantly higher in isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury when compared to both cyclosporine A treated and sham groups. Necrosis percentage was significantly higher in propofol-anesthetized animals subjected to renal ischemia-reperfusion injury. The percentage of intact cells was lower in both, isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury.Conclusion:
In a model of ischemia-reperfusion-induced renal injury, cyclosporine A, 5 m.kg-1, administered five minutes before renal reperfusion in rats with acute-induced hyperglycemia under either isoflurano or propofol anesthesia, attenuated early apoptosis and preserved viability in renal tubular cells, regardless of the anesthetic used.Asunto(s)
Animales Masculino Daño por Reperfusión/prevención & control Ciclosporina/farmacología Apoptosis/efectos de los fármacos Sustancias Protectoras/farmacología Hiperglucemia/fisiopatología Riñón/efectos de los fármacos Premedicación Factores de Tiempo Daño por Reperfusión/complicaciones Distribución Aleatoria Propofol/farmacología Supervivencia Celular/efectos de los fármacos Reproducibilidad de los Resultados Resultado del Tratamiento Ratas Wistar Anestésicos Intravenosos/farmacología Anestésicos por Inhalación/farmacología Citometría de Flujo Isquemia/prevención & control Isoflurano/farmacología Riñón/irrigación sanguínea Riñón/patología Necrosis/prevención & control
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