Abstract
Purpose: Topical
hypothermia and local
ischemic preconditioning have been shown to reduce renal
ischemia -
reperfusion (I/R)
injury individually. We examined whether combination of both
strategies lessens renal I/R
injury .
Methods: Post right
nephrectomy , 40
male Wistar rats were randomly assigned to five experimental
protocols performed in the left
kidney topical
hypothermia without
ischemia (TH),
warm ischemia (IR),
ischemic preconditioning followed by
warm ischemia (IPC+IR),
cold ischemia (TH+IR), and
ischemic preconditioning followed by
cold ischemia (IPC+TH+IR). Eight randomly assigned right
kidneys constituted the
control group . After 240 min of
reperfusion , the left
kidney was retrieved to evaluate histological changes,
lipid peroxidation and
antioxidant enzymes activity.
Serum was collected to evaluate
urea and
creatinine .
Results: IPC+TH+IR group revealed no difference to any other group subjected to
ischemia in relation to histological changes,
lipid peroxidation and
antioxidant enzymes activity.
Creatinine was lower in IPC+TH+IR group compared with IPC+IR, but showed no difference compared to TH+IR group.
Conclusions: Combination of local
ischemic preconditioning (IPC) and topical
hypothermia conferred no
protection in renal I/R
injury . Moreover, local IPC solely followed by
warm ischemia impaired renal function more than
warm ischemia alone.