ABSTRACT
Hydrogen peroxide is a common
reactive oxygen species involved in the catalytic mechanism though it is toxic to
cells due to its oxidative
nature. This
work investigates the effects of
hydrogen peroxide induced
oxidative damage on
bone mineral density and mechanical properties of
bone which is primarily a composite material composed primarily of
collagen fibers and biominerals.
Sheep leg bones were exposed to
hydrogen peroxide for a week.
Bone mineral density was measured by using dual energy
X-ray absorptiometry. Compressive modulus tests were applied to
bone in order to determine mechanical properties. Our study shows that the
hydrogen peroxide induced
oxidative stress has negative effects on
bone mineral density and stiffness. We observed higher control curve slopes than that of
hydrogen peroxide curves which account for lesser stiffness values in the exposed
tissue (p<0.05).