Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
Marinheiro, Juliana Cristina; Sanalios, Roberta Braga; Santos, Daniela Carvalho dos; Costa, Cristovão Alves da; Hársi, Charlotte Marianna.
Mem. Inst. Oswaldo Cruz;
104(1): 118-120, Feb. 2009. ilus
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| LILACS | ID: lil-507216
Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
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