The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
Andrade-Neto, Valter Viana; Matos-Guedes, Herbert Leonel de; Gomes, Daniel Cláudio de Oliveira; Canto-Cavalheiro, Marilene Marcuzzo do; Rossi-Bergmann, Bartira; Torres-Santos, Eduardo Caio.
Mem. Inst. Oswaldo Cruz;
107(3): 416-419, May 2012. graf
Artículo
en Inglés
| LILACS | ID: lil-624025
Ketoconazole is a clinically safe antifungal agent that also inhibits the growth of Leishmania spp. A study was undertaken to determine whether Leishmania parasites are prone to becoming resistant to ketoconazole by upregulating C14-demethylase after stepwise pharmacological pressure. Leishmania amazonensis promastigotes [inhibitory concentration (IC)50 = 2 µM] were subjected to stepwise selection with ketoconazole and two resistant lines were obtained, La8 (IC50 = 8 µM) and La10 (IC50 = 10 µM). As a result, we found that the resistance level was directly proportional to the C14-demethylase mRNA expression level; we also observed that expression levels were six and 12 times higher in La8 and La10, respectively. This is the first demonstration that L. amazonensis can up-regulate C14-demethylase in response to drug pressure and this report contributes to the understanding of the mechanisms of parasite resistance.
Biblioteca responsable:
BR1.1
Texto completo