A simple protocol is described for the
silver staining of polyacrylamide gradient
gels used for the
separation of restriction fragments of
kinetoplast DNA [schizodeme
analysis of trypanosomatids (Morel et al., 1980)]. The
method overcomes the problems of non-
uniform staining and strong background
color which are frequently encountered when conventional
protocols for
silver staining of linear
gels. The
method described has proven to be of general applicability for
DNA,
RNA and
protein separations in gradient
gels