Analysis of vertebrate SCL loci identifies conserved enhancers.
Nat Biotechnol
; 18(2): 181-6, 2000 Feb.
Article
en En
| MEDLINE
| ID: mdl-10657125
ABSTRACT
The SCL gene encodes a highly conserved bHLH transcription factor with a pivotal role in hemopoiesis and vasculogenesis. We have sequenced and analyzed 320 kb of genomic DNA composing the SCL loci from human, mouse, and chicken. Long-range sequence comparisons demonstrated multiple peaks of human/mouse homology, a subset of which corresponded precisely with known SCL enhancers. Comparisons between mammalian and chicken sequences identified some, but not all, SCL enhancers. Moreover, one peak of human/mouse homology (+23 region), which did not correspond to a known enhancer, showed significant homology to an analogous region of the chicken SCL locus. A transgenic Xenopus reporter assay was established and demonstrated that the +23 region contained a new neural enhancer. This combination of long-range comparative sequence analysis with a high-throughput transgenic bioassay provides a powerful strategy for identifying and characterizing developmentally important enhancers.
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Bases de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Vertebrados
/
Proteínas Proto-Oncogénicas
/
Elementos de Facilitación Genéticos
/
Secuencia Conservada
/
Proteínas de Xenopus
/
Proteínas de Unión al ADN
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Reino Unido