Myostatin regulates cell survival during C2C12 myogenesis.
Biochem Biophys Res Commun
; 280(2): 561-6, 2001 Jan 19.
Article
en En
| MEDLINE
| ID: mdl-11162556
ABSTRACT
During the myogenic process in vitro, proliferating myoblasts withdraw irreversible from the cell cycle, acquire an apoptosis-resistant phenotype, and fuse into mature myotubes. The key factor regulating both myocyte cell cycle exit and viability during this transition is the the cyclin-dependent kinase inhibitor p21(cip1). Here we show that the expression of myostatin, a TGF-beta superfamily member known to act as a negative regulator of muscle growth, is upregulated in the course of C2C12 cells myogenesis. We also show that transient transfection of C2C12 myobasts with an expression vector encoding mouse myostatin cDNA efficiently inhibits cell proliferation. Paradoxically, myostatin cDNA overexpression also enhances the survival of differentiating C2C12 myocytes, probably by a mechanism involving, at least in part, upregulation of p21(cip1) mRNA. Our results suggest that myostatin role in myogenesis is more complex than initially suggested and involves another level of regulation apart from inhibition of myoblast proliferation.
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Bases de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Supervivencia Celular
/
Factor de Crecimiento Transformador beta
/
Músculos
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2001
Tipo del documento:
Article
País de afiliación:
España