Characterization of attenuated Renibacterium salmoninarum strains and their use as live vaccines.
Dis Aquat Organ
; 44(2): 121-6, 2001 Mar 09.
Article
en En
| MEDLINE
| ID: mdl-11324812
ABSTRACT
Two nutritionally mutant strains of Renibacterium salmoninarum (Rs) were isolated that grew on tryticase soy agar (Rs TSA1) or brain heart infusion agar (Rs BHI1). These 2 strains could be continuously cultured on these media, whereas typical R. salmoninarum would only grow on KDM-2 agar. We determined no other phenotypic difference that could be used to distinguish them from wild-type R. salmoninarum. Both strains were found to be avirulent when 5 x 10(6) bacteria were intraperitoneally (i.p.) injected into Atlantic salmon. Rs TSA1, Rs BHI1, and Rs MT-239 (a R. salmoninarum strain previously shown to be attenuated) were tested as live vaccines in 2 separate trials. The best protection was seen with Rs TSA1. Vaccinated Atlantic salmon had relative percent survival (RPS) of 50 at 74 d post-challenge in Trial 1 and 76 at 60 d post-challenge in Trial 2. In both trials, 100% of the control salmon died from bacterial kidney disease (BKD) (within 40 d for Trial 1 and 50 d for Trial 2) after i.p. challenge with 5 x 10(6) live cells of the virulent isolate Rs Margaree.
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Bases de datos:
MEDLINE
Asunto principal:
Vacunas Bacterianas
/
Vacunas Atenuadas
/
Infecciones por Bacterias Grampositivas
/
Enfermedades de los Peces
/
Micrococcaceae
Límite:
Animals
Idioma:
En
Revista:
Dis Aquat Organ
Asunto de la revista:
BIOLOGIA
/
MEDICINA VETERINARIA
/
MICROBIOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos