Escherichia coli strains from edema disease: O serogroups, and genes for Shiga toxin, enterotoxins, and F18 fimbriae.
Vet Microbiol
; 80(3): 227-33, 2001 Jun 06.
Article
en En
| MEDLINE
| ID: mdl-11337138
ABSTRACT
The objectives of the research were to determine the presence of the gene sequences for Shiga Toxin 2e (Stx2e), enterotoxins (ST-I, ST-II and LT-I), and F18 fimbriae in 144 Escherichia coli strains isolated from pigs with edema disease; to assess the ability of stx2e(+) strains to produce Stx2e; and to determine the O serogroups of the E. coli strains. Presence of the genes was determined by polymerase chain reaction (PCR), production of Stx2e was assessed by cytotoxicity for Vero and Hela cells, O serogroups were identified by agglutination with specific antisera. Of the 144 strains tested, 99 were stx2e(+) by PCR, but only 45 of these were Stx2e(+) in the cell culture assays. Among the 99 stx2e(+) strains, PCR detected the genes for F18ab, ST-I, ST-II, LT-I in 76, 40, 31 and 16 strains, respectively. Forty-one of the 99 sxt2e(+) strains belonged to O group 139; the rest did not belong to the classical edema disease O serogroups. It is likely that the enterotoxins, whose genes were detected at high frequency, are responsible for diarrhea seem in pigs with edema disease in Brazil.
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Bases de datos:
MEDLINE
Asunto principal:
Fimbrias Bacterianas
/
Antígenos O
/
Toxinas Shiga
/
Edematosis Porcina
/
Escherichia coli
/
Infecciones por Escherichia coli
Límite:
Animals
/
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Vet Microbiol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Brasil