Optimizing bioconversion pathways through systems analysis and metabolic engineering.
Proc Natl Acad Sci U S A
; 99(4): 1801-6, 2002 Feb 19.
Article
en En
| MEDLINE
| ID: mdl-11854482
ABSTRACT
We demonstrate a general approach for metabolic engineering of biocatalytic systems comprising the uses of a chemostat for strain improvement and radioisotopic tracers for the quantification of pathway fluxes. Flux determination allows the identification of target pathways for modification as validated by subsequent overexpression of the corresponding gene. We demonstrate this method in the indene bioconversion network of Rhodococcus modified for the overproduction of 1,2-indandiol, a key precursor for the AIDS drug Crixivan.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Ingeniería Biomédica
/
Industria Farmacéutica
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos