Improved ceramic-based multisite microelectrode for rapid measurements of L-glutamate in the CNS.
J Neurosci Methods
; 119(2): 163-71, 2002 Sep 30.
Article
en En
| MEDLINE
| ID: mdl-12323420
ABSTRACT
This paper describes improvements and further characterization of a ceramic-based multisite microelectrode for in vivo measurements of L-glutamate. Improvements include increased recording area, insulation deposition using photolithography for more uniform recording sites and forming the microelectrodes using a diamond saw providing smoother microelectrode edges. The new microelectrodes are triangular in shape, 1 cm in length and taper from 1 mm to a 2-5 microm tip. Details on performing in vivo measurements are given, including microelectrode preparation, pitfalls of the recording method and approaches to enhance reproducibility of the technique. The detection limit for L-glutamate was lowered to approximately 0.5 microM and a self-referencing recording technique was utilized to remove interferents as well as decrease noise. Applications of the microelectrodes to study L-glutamate uptake and release in rat prefrontal cortex, cortex, cerebellum and striatum are included.
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Bases de datos:
MEDLINE
Asunto principal:
Sistema Nervioso Central
/
Cerámica
/
Microdiálisis
/
Ácido Glutámico
Límite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos