Hemin stimulation of cAMP production in human lymphocytes.
FEBS Lett
; 303(2-3): 242-6, 1992 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-1318850
ABSTRACT
Hemin stimulates cAMP production in human peripheral blood mononuclear cells (PBMC). The kinetics are similar to that of hormone-induced cAMP generation, namely a rapid effect followed by a desensitization phase. Several experimental findings suggest that prostaglandins do not mediate this effect. First, macrophage depleted T and B cells purified by erythrocyte-rosetting were as responsive as unfractionated PBMC to hemin. Second, indomethacin, an inhibitor of prostaglandin synthesis, and meclofenamate, a prostaglandin E2 receptor antagonist, had no effect on hemin stimulated cAMP production. In addition, propranolol, a beta-adrenergic receptor antagonist, had no effect on hemin-stimulated cAMP production. We also examined structural analogues of hemin. Among the metalloporphyrins (Fe, Ni, Co, Zn and Sn) and protoporphyrin IX tested only hemin (Fe-protoporphyrin) was active in stimulating cAMP production. No correlation was found between the ability of metalloporphyrins to stimulate cAMP production and their ability to generate H2O2. The data indicate that hemin stimulates cAMP production by directly affecting lymphocytes and that prostaglandins do not mediate this effect.
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Bases de datos:
MEDLINE
Asunto principal:
Linfocitos
/
AMP Cíclico
/
Hemina
Límite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
1992
Tipo del documento:
Article