A DNA probe specific to pathogenic Entamoeba histolytica.
Arch Med Res
; 23(2): 271-5, 1992.
Article
en En
| MEDLINE
| ID: mdl-1340313
ABSTRACT
A DNA sequence, IE-gen1 (3.1 kb), was isolated from the pathogenic strain of E. histolytica NIH-200. IE-gen1 was identified by the subtractive hybridization of a genomic library to a cDNA probe prepared from NIH-200 trophozoites. The IE-gen1 probe specifically detected pathogenic E. histolytica in slot blots of genomic DNA and Northern blots, but not other Entamoeba species and additional human parasites. This genomic probe could detect with complete specificity DNA from about 10(3) organisms. The IE-gen1 probe could be related to highly specialized loci in pathogenic E. histolytica, and is likely to be a valuable DNA reagent for clinical diagnosis and epidemiological investigations.
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Bases de datos:
MEDLINE
Asunto principal:
Sondas de ADN
/
ADN Protozoario
/
Entamoeba histolytica
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Arch Med Res
Asunto de la revista:
MEDICINA
Año:
1992
Tipo del documento:
Article
País de afiliación:
Reino Unido