Comparison of in vivo lymphocyte proliferation between allogeneic and xenogeneic heart transplantation in mice.
Microsurgery
; 23(5): 498-502, 2003.
Article
en En
| MEDLINE
| ID: mdl-14558010
ABSTRACT
There are controversial in vitro data comparing the strength of the cellular immune response between allogeneic and xenogeneic stimulator/responder combinations. The present study therefore compares in vivo lymphocyte proliferation using heart transplantation (HTx) models in mice. Heterotopic HTx into BALB/c mice was performed using donor organs from mice (BALB/c and C57BL/6) or Lewis rats. Intraperitoneally given bromodeoxyuridine (BrdU) was incorporated into the DNA and was subsequently analyzed by flow cytometry. On postoperative days 3 and 5, proliferation of splenocytes, CD4(+) T-lymphocytes, and CD19(+) B-lymphocytes was significantly higher after xenogeneic than after allogeneic and isogeneic HTx. No significant difference was observed when proliferation of CD8(+) lymphocytes was determined. The increased in vivo proliferation after xenotransplantation may reflect an earlier and probably stronger cellular immune response compared to allogeneic transplantation. The higher CD4(+) lymphocyte proliferation underscores the importance of this cell population in xenograft rejection.
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Bases de datos:
MEDLINE
Asunto principal:
Trasplante Heterólogo
/
Trasplante Homólogo
/
División Celular
/
Subgrupos Linfocitarios
/
Trasplante de Corazón
Límite:
Animals
Idioma:
En
Revista:
Microsurgery
Año:
2003
Tipo del documento:
Article
País de afiliación:
Alemania