[Anti-mesangial cell autoantibodies and their target antigens in lupus nephritis].
Zhonghua Nei Ke Za Zhi
; 42(12): 851-3, 2003 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-14728876
ABSTRACT
OBJECTIVE:
Anti-mesangial cell autoantibodies (anti-MC) have been reported in patients with IgA nephropathy and Henoch-Schoenlein purpura. The aim of the current study was to identify anti-MC autoantibodies in sera from patients with lupus nephritis (LN).METHODS:
96 serum samples were collected from 96 patients with renal biopsy proven LN. Anti-MC autoantibodies were examined by cellular ELISA and Western blot analysis. In vitro cultured human mesangial cells were used as cell substrate in ELISA and the their soluble proteins in non-reducing conditions were used as antigens in Western-blot analysis.RESULTS:
37.5% and 97.9% of the serum samples from patients with LN had anti-MC autoantibodies in cellular ELISA and Western blot analysis respectively, and 12 protein bands could be blotted by the LN sera in Western blot analysis. The percentage of anti-63 000 autoantibody positive samples was significant higher in female patients than that in the male (59.8% vs 21.4%, P < 0.01). The prevalence of anti-74 000, 46 000, and 36 000 autoantibodies were significantly higher in patients with hematuria than that in patients without hematuria (36.8% vs 5.0%, P < 0.01; 34.2% vs 10.0%, P < 0.05; 31.6% vs 5.0%, P < 0.05; respectively). The prevalence of anti-63 000 autoantibodies was also significantly higher in patients with ANA than that in patients without (67.6% vs 16.7%, P < 0.001). The prevalence of anti-18 000 autoantibodies was significantly higher in patients with anti-dsDNA antibodies than that in patients without (61.5% vs 34.4%, P < 0.05).CONCLUSIONS:
There are heterogeneous anti-MC autoantibodies in sera from patients with LN. Anti-MC autoantibodies may have a pathogenic role in lupus nephritis and some anti-MC autoantibodies may be associated with different clinical manifestations.
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Bases de datos:
MEDLINE
Asunto principal:
Autoanticuerpos
/
Nefritis Lúpica
Tipo de estudio:
Risk_factors_studies
Límite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Nei Ke Za Zhi
Año:
2003
Tipo del documento:
Article
País de afiliación:
China