Calcium transport and adenosine triphosphatase activities of erythrocyte membranes in congenital spherocytosis.
Scand J Clin Lab Invest
; 38(2): 121-5, 1978 Apr.
Article
en En
| MEDLINE
| ID: mdl-148726
ABSTRACT
Calcium transport from red cells was measured in seventeen patients with congenital or hereditary spherocytosis (HS). The efflux remained at a lower level in resealed ghost cells of patients than in normal cells both in the presence and absence of adenosine triphosphate (ATP). We studied the activities of Ca2+,Mg2+-ATPase, ouabain-sensitive Na+,K+-ATPase, Mg2+-ATPase and Ca2+-(spectrin-)ATPase in cell membranes prepared by washing the cells with hypotonic medium. The mean +/-SD Ca2+,Mg2+-ATPase/Mg2+-ATPase of HS patients was 3.34 +/- 1.06, and 2.81 +/- 0.42 in control subjects. Na+,K+-ATPase/Mg2+-ATPase was 2.38 +/- 0.38 in HS cells compared to 2.01 +/- 0.41 in normal cells. Ca2+-ATPase/Mg2+-ATPase of HS membranes was 0.57 +/- 0.18 and the control value 0.43 +/- 0.08. These data indicate calcium retention in the erythrocytes of HS patients in spite of increases in Ca2+,Mg2+-ATPase activity in the majority of patients.
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Bases de datos:
MEDLINE
Asunto principal:
Esferocitosis Hereditaria
/
Calcio
/
Adenosina Trifosfatasas
/
Membrana Eritrocítica
/
Eritrocitos
Límite:
Humans
Idioma:
En
Revista:
Scand J Clin Lab Invest
Año:
1978
Tipo del documento:
Article