The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cells.
Mol Cell Biol
; 24(10): 4207-20, 2004 May.
Article
en En
| MEDLINE
| ID: mdl-15121842
ABSTRACT
The Sox-2 gene is expressed in embryonic stem (ES) cells and neural stem cells. Two transcription enhancer regions, Sox-2 regulatory region 1 (SRR1) and SRR2, were described previously based on their activities in ES cells. Here, we demonstrate that these regulatory regions also exert their activities in neural stem cells. Moreover, our data reveal that, as in ES cells, both SRR1 and SRR2 show their activities rather specifically in multipotent neural stem or progenitor cells but cease to function in differentiated cells, such as postmitotic neurons. Systematic deletion and mutation analyses showed that the same or at least overlapping DNA elements of SRR2 are involved in its activity in both ES and neural stem or progenitor cells. Thus, SRR2 is the first example of an enhancer in which a single regulatory core sequence is involved in multipotent-state-specific expression in two different stem cells, i.e., ES and neural stem cells.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas Nucleares
/
Células Madre Multipotentes
/
Proteínas de Unión al ADN
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Mol Cell Biol
Año:
2004
Tipo del documento:
Article
País de afiliación:
Japón