FMRFamide tagging--how an ionotropic receptor can be used to measure peptide secretion.
Methods
; 33(4): 295-301, 2004 Aug.
Article
en En
| MEDLINE
| ID: mdl-15183178
ABSTRACT
In this chapter, we describe a technique, FMRFamide tagging, that in principle can be used to measure the release of any sequenced neuropeptide. The method relies upon the addition of an "electrophysiologically active" tag to the prohormone that encodes the neuropeptide of interest. Secretion of the electrophysiological tag (and thus the peptide of interest) is detected by activation of the ionotropic "tag receptor." Both the tagged prohormone and the tag receptor are expressed in the cell type under investigation. Since the tag and the neuropeptide of interest are on the same prohormone they are co-secreted and thus secretion of the tag reflects the co-secretion of the neuropeptide of interest. This method can be used to detect neuropeptide secretion on a millisecond timescale.
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Bases de datos:
MEDLINE
Asunto principal:
Neuropéptidos
/
Receptores de Glutamato
/
Receptores de Péptidos de Invertebrados
/
FMRFamida
Límite:
Animals
Idioma:
En
Revista:
Methods
Asunto de la revista:
BIOQUIMICA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos