Epigenetic activation of the 5-hydroxytryptamine (serotonin) receptor 2C in embryonal carcinoma cells is DNA replication-dependent.
Exp Cell Res
; 298(1): 262-7, 2004 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-15242780
ABSTRACT
The epigenetic states of key regulatory genes must be altered to drive cell fate decisions in differentiating cells. This process must be coupled, at least transiently, to the DNA replication machinery. Only a few genes, however, have been shown to require DNA replication for their activation or repression upon induction of differentiation. We have developed a methodology for examining how gene expression is coupled to cell division during the early stages of differentiation of embryonal carcinoma (EC) cells. Using this approach, we find that the expression of the 5-hydroxytryptamine (serotonin) receptor 2C (Htr2c) is strongly increased in the second division after all-trans retinoic acid addition. We propose that the epigenetic activation of Htr2c in EC cells results from a chromatin remodeling process that requires at least two passages through S phase.
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Bases de datos:
MEDLINE
Asunto principal:
Células Madre Neoplásicas
/
Diferenciación Celular
/
Regulación del Desarrollo de la Expresión Génica
/
Receptor de Serotonina 5-HT2C
/
Epigénesis Genética
/
Replicación del ADN
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Exp Cell Res
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos