Cell-specific protein profiling in Arabidopsis thaliana trichomes: identification of trichome-located proteins involved in sulfur metabolism and detoxification.
Phytochemistry
; 65(11): 1641-9, 2004 Jun.
Article
en En
| MEDLINE
| ID: mdl-15276459
ABSTRACT
Metabolite, protein, and transcript analysis at the cellular level gives unparalleled insight into the complex roles tissues play in the plant system. However, while capillary electrophoresis and PCR amplification strategies make the profiling of metabolites and transcripts in specific cell types possible, the profiling of proteins in small samples represents a bottleneck. Here for the first time protein profiling has been achieved in a specific plant cell type The application of specific cell sampling and shotgun peptide sequencing (nano LC/MS/MS) resulted in the identification of 63 unique proteins from pooled Arabidopsis trichome cells. A complete S-adenosylmethionine pathway cluster, two S-adenosylmethionine synthase isoforms, a glutathione S-conjugate translocator and other proteins involved in sulfur metabolism and detoxification are shown to be present in these cells, in agreement with previous work done at the level of trichome transcript analysis. The technology described here brings the simultaneous identification and localization of physiologically relevant cellular proteins within reach.
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Bases de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Azufre
/
Arabidopsis
/
Perfilación de la Expresión Génica
/
Proteínas de Arabidopsis
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Phytochemistry
Año:
2004
Tipo del documento:
Article
País de afiliación:
Alemania