Lipid effects on neutrophil calcium signaling induced by opsonized particles: platelet activating factor is only part of the story.
Clin Nutr
; 23(4): 623-30, 2004 Aug.
Article
en En
| MEDLINE
| ID: mdl-15297099
ABSTRACT
BACKGROUND & METHODS:
Total parenteral nutrition is frequently used in clinical practice to improve the nutritional status of patients. However, the risk for infectious complications remains a drawback in which immune-modulating effects of the lipid component may play a role. To characterize these lipid effects we investigated neutrophil activation by opsonized yeast particles under influence of lipid emulsions derived from fish oil (VLCT), olive oil (LCT-MUFA), soybean oil (LCT), and a physical mixture of coconut and soybean oil (LCT-MCT).RESULTS:
Serum-treated zymosan (STZ) evoked a biphasic increase in cytosolic Ca2+ concentration ([Ca2+]c) with an initial slow rise that turned into a second fast rise until a plateau was reached. LCT-MCT (5 mM) pretreatment markedly increased the rate of [Ca2+]c rise during the initial phase, abolished the second phase and lowered the plateau. These effects of LCT-MCT were mimicked by the protein kinase C (PKC) activating phorbol ester PMA. LCT, LCT-MUFA and VLCT, on the other hand, decreased the rate of [Ca2+]c rise during both phases and lowered the plateau. The platelet-activating factor (PAF) receptor antagonist WEB 2086 inhibited the second phase, demonstrating that PAF acts as an intercellular messenger in STZ-induced Ca2+ mobilization, but did not interfere with the stimulatory effect of LCT-MCT or PMA on the initial rate of [Ca2+]c rise.CONCLUSIONS:
Structurally different lipids act only in part through PAF to distinctively modulate neutrophil calcium signaling in response to activation by opsonized particles.
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Bases de datos:
MEDLINE
Asunto principal:
Factor de Activación Plaquetaria
/
Calcio
/
Señalización del Calcio
/
Emulsiones Grasas Intravenosas
/
Neutrófilos
Límite:
Humans
Idioma:
En
Revista:
Clin Nutr
Año:
2004
Tipo del documento:
Article
País de afiliación:
Países Bajos