Suppression of homologous and homeologous recombination by the bacterial MutS2 protein.
Mol Cell
; 17(1): 113-20, 2005 Jan 07.
Article
en En
| MEDLINE
| ID: mdl-15629722
ABSTRACT
In addition to their role in DNA repair, recombination events are associated with processes aimed at providing the genetic variability needed for adaptation and evolution of a population. In bacteria, recombination is involved in the appearance of new variants by allowing the incorporation of exogenous DNA or the reshuffling of endogenous sequences. Here we show that HpMutS2, a protein belonging to the MutS2 family in Helicobacter pylori, is not involved in mismatch repair but inhibits homologous and homeologous recombination. Disruption of HpmutS2 leads to an increased efficiency of exogenous DNA incorporation. HpMutS2 has a selective affinity for DNA structures mimicking recombination intermediates with no specificity for homoduplex DNA or mismatches. The purified protein has an ATPase activity stimulated by the same DNA structures. Finally, we show that HpMutS2 inhibits DNA strand exchange reactions in vitro. Thus, MutS2 proteins are candidates for controlling recombination and therefore genetic diversity in bacteria.
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Bases de datos:
MEDLINE
Asunto principal:
Recombinación Genética
/
Proteínas Bacterianas
/
Helicobacter pylori
/
Adenosina Trifosfatasas
/
Proteínas de Unión al ADN
Idioma:
En
Revista:
Mol Cell
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2005
Tipo del documento:
Article
País de afiliación:
Francia