[Binding and inhibition of adeno-associated virus Rep78 protein with hepatitis B virus C promoter].
Zhonghua Gan Zang Bing Za Zhi
; 13(3): 187-9, 2005 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-15760551
ABSTRACT
OBJECTIVES:
Adeno-associated virus (AAV) Rep78 is known for its inhibitory effects on replication of several viruses and oncogenes transformations. The study was to investigate the effect of Rep78 on hepatitis B virus C (HBV-C) gene and the mechanism of it.METHODS:
HBV-C promoter and HBV-C gene with its promoter were amplified by PCR and labeled with 32P-ATP. Electrophoretic mobility shift assay (EMSA) and in vitro transcription were utilized to detect the binding of MBP-Rep78 with HBV-C promoter and the transcription of HBV-C gene.RESULTS:
EMSA showed that by increasing the amount of Rep78 protein from 0.1 microg to 1.0 microg, the binding bands got stronger in a dose-dependent manner. In addition, Rep78 antibody was used to certify the specificity of this binding. The compound of Rep78, Rep78 antibody and HBV-C promoter were seen as super shift bands in EMSA. Meanwhile, HBV-C gene transcription was significantly inhibited by in vitro transcription which meant that Rep78 could not only bind with HBV-C promoter, but also could inhibit the transcription of HBV-C gene.CONCLUSION:
AAV Rep78 could inhibit the transcription of HBV-C gene through its binding with HBV-C promoter.
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Bases de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Proteínas Virales
/
Virus de la Hepatitis B
/
Regiones Promotoras Genéticas
/
Dependovirus
/
Proteínas de Unión al ADN
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
China