CXCL16 is a novel angiogenic factor for human umbilical vein endothelial cells.
Biochem Biophys Res Commun
; 331(4): 1295-300, 2005 Jun 17.
Article
en En
| MEDLINE
| ID: mdl-15883016
ABSTRACT
CXCL16 is a unique chemokine with characteristics as a receptor for phosphatidylserine and oxidized low density lipoproteins in macrophages, and is involved in the accumulation of cellular cholesterol during atherosclerotic lesion development. In this study, we report a new function of CXCL16 as a novel angiogenic factor in human umbilical vein endothelial cells (HUVEC). CXCL16 stimulated proliferation and chemotaxis of HUVEC in a dose-dependent manner, reaching a maximum at 1 nM. CXCL16 also significantly induced tube formation of HUVEC on Matrigel. Further, exposure of HUVEC to CXCL16 led to a time- and dose-dependent activation of mitogen-activated protein kinase (ERK1/2), which was completely inhibited by a mitogen-activated protein kinase kinase inhibitor, PD98059. Proliferation and tube formation in response to CXCL16 were also blocked by the pretreatment with PD98059, but not CXCL16-induced chemotaxis. Thus, our data indicate that CXCL16 may act as a novel angiogenic factor for HUVEC and that ERK is involved as an important signaling molecule to mediate its angiogenic effects.
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Bases de datos:
MEDLINE
Asunto principal:
Venas Umbilicales
/
Endotelio Vascular
/
Receptores Inmunológicos
/
Neovascularización Fisiológica
/
Quimiocinas CXC
/
Proteínas de la Membrana
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2005
Tipo del documento:
Article
País de afiliación:
Japón