Techniques for antifungal susceptibility testing of Candida albicans biofilms.
Methods Mol Med
; 118: 71-9, 2005.
Article
en En
| MEDLINE
| ID: mdl-15888936
ABSTRACT
Candida albicans is capable of forming biofilms on a variety of inert and biological surfaces. Cells in biofilms display phenotypic properties that are radically different from their free-floating planktonic counterparts, including their recalcitrance to antimicrobial agents. Consequently, Candida biofilm-associated infections are difficult to treat and to fully eradicate with standard antifungal therapy. Here, we describe a simple, fast, inexpensive and highly reproducible microtiter plate-based assay for the antifungal susceptibility testing of C. albicans biofilms. Because of its simplicity, compatibility with widely available 96-well microplate platform, high throughput, and automation potential, this assay represents an important tool towards the standardization of in vitro antifungal susceptibility testing of fungal biofilms.
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Bases de datos:
MEDLINE
Asunto principal:
Candida albicans
/
Pruebas de Sensibilidad Microbiana
/
Biopelículas
/
Antifúngicos
Idioma:
En
Revista:
Methods Mol Med
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2005
Tipo del documento:
Article
País de afiliación:
Reino Unido